scholarly journals Purification and characterization of mouse fetal liver epithelial cells with highin vivorepopulation capacity

Hepatology ◽  
2005 ◽  
Vol 42 (1) ◽  
pp. 130-139 ◽  
Author(s):  
Dirk Nierhoff ◽  
Atsushi Ogawa ◽  
Michael Oertel ◽  
Yuan-Qing Chen ◽  
David A. Shafritz
Keyword(s):  
Epithelial Cells ◽  
Fetal Liver ◽  
Purification And Characterization ◽  
Liver Epithelial Cells

Characterization of liver epithelial cells transfected withmyc and/orras oncogenes

1991 ◽  
Vol 36 (5) ◽  
pp. 642-652 ◽  
Author(s):  
Stephen C. Strom ◽  
John B. Faust ◽  
Erika Cappelluti ◽  
Robert B. Harris ◽  
Narendra D. Lalwani
Keyword(s):  
Epithelial Cells ◽  
Liver Epithelial Cells

The same γ-glutamyl transpeptidase RNA species is expressed in fetal liver, hepatic carcinomas, and ras T24-transformed rat liver epithelial cells

1992 ◽  
Vol 5 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Geetha M. Habib ◽  
Sridharan Rajagopalan ◽  
Andrew K. Godwin ◽  
Russell M. Lebovitz ◽  
Michael W. Lieberman
Keyword(s):  
Epithelial Cells ◽  
Rat Liver ◽  
Fetal Liver ◽  
Liver Epithelial Cells ◽  
Rat Liver Epithelial Cells ◽  
Glutamyl Transpeptidase

scholarly journals Purification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor

Blood ◽  
1993 ◽  
Vol 82 (3) ◽  
pp. 762-770 ◽  
Author(s):  
D Orlic ◽  
R Fischer ◽  
S Nishikawa ◽  
AW Nienhuis ◽  
DM Bodine
Keyword(s):  
Fetal Liver ◽  
Biochemical Properties ◽  
Tyrosine Kinase Receptor ◽  
Low Density ◽  
Purification And Characterization ◽  
Hematopoietic Stem ◽  
Density Fractions ◽  
Kit Receptor ◽  
Stem Cell Populations

Mouse pluripotent hematopoietic stem cells (PHSC) were fractionated based on size and density using counterflow centrifugal elutriation (CCE). These heterogeneous PHSC populations were further enriched by subtraction of cells with lineage-specific markers (Lin-) followed by positive sorting for c-kit expression. The cells were characterized for their functional and biochemical properties. We defined a subpopulation of c-kit-positive cells that expressed high numbers of c-kit receptors (c-kitBR). One hundred c-kitBR cells from either low- or higher-density fractions were sufficient to repopulate the lymphohematopoietic system in WBB6F1-W/Wv (W/Wv) recipients, whereas no PHSC were found in cells with low (c-kitDULL) or no (c-kitNEG) c-kit expression. Lin- c-kitBR cells were separated into RhoDULL and RhoBR subsets based on their ability to efflux rhodamine 123 (Rho). The PHSC were concentrated in Lin- c-kitBR RhoDULL cells and the number of Lin- c-kitBR RhoBR cells correlated directly with the number of day 12 colony-forming unit- spleen (CFU-S12) in each fraction. We were not able to enrich further for PHSC using monoclonal antibodies to the cell-surface markers AA4.1 or CD4, which have been used by others to isolate PHSC. The small, low- density Lin- c-kitBR subset contained PHSC and few CFU-S12. This enabled us to assay PHSC for expression of the flk-2 gene, which encodes a tyrosine kinase receptor present on fetal liver PHSC. Purified RNA from the low-density Lin- c-kitBR subset did not contain flk-2 mRNA. We suggest that AA4.1, CD4 and flk-2 are expressed as stage- specific markers on PHSC in cell cycle.

Effects of growth factors on the growth and differentiation of mouse fetal liver epithelial cells in primary cultures

2005 ◽  
Vol 20 (6) ◽  
pp. 857-864 ◽  
Author(s):  
TAKAKO KAWASAKI ◽  
SHINJI TAMURA ◽  
SHINICHI KISO ◽  
YOSHINORI DOI ◽  
YUICHI YOSHIDA ◽  
...  
Keyword(s):  
Growth Factors ◽  
Epithelial Cells ◽  
Fetal Liver ◽  
Primary Cultures ◽  
Liver Epithelial Cells

scholarly journals The purification and characterization of fetal liver hematopoietic stem cells.

1995 ◽  
Vol 92 (22) ◽  
pp. 10302-10306 ◽  
Author(s):  
S. J. Morrison ◽  
H. D. Hemmati ◽  
A. M. Wandycz ◽  
I. L. Weissman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Fetal Liver ◽  
Purification And Characterization ◽  
Hematopoietic Stem
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