Identification of a new marker of hepatocellular carcinoma by serum protein profiling of patients with chronic liver diseases

Hepatology ◽  
2004 ◽  
Vol 41 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Valérie Paradis ◽  
Francoise Degos ◽  
Delphine Dargère ◽  
Nanou Pham ◽  
Jacques Belghiti ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Serum Protein ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Protein Profiling ◽  
Chronic Liver Diseases

scholarly journals Micro-RNAs in hepatitis B virus-related chronic liver diseases and hepatocellular carcinoma

2018 ◽  
Vol 10 (9) ◽  
pp. 558-570 ◽  
Author(s):  
Evangelista Sagnelli ◽  
Nicoletta Potenza ◽  
Lorenzo Onorato ◽  
Caterina Sagnelli ◽  
Nicola Coppola ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Chronic Liver Diseases ◽  
Micro Rnas ◽  
B Virus

scholarly journals Quantitative Analysis of Aldolase A mRNA in Liver Discriminates between Hepatocellular Carcinoma and Cirrhosis

2000 ◽  
Vol 46 (7) ◽  
pp. 901-906 ◽  
Author(s):  
Giuseppe Castaldo ◽  
Giuseppe Calcagno ◽  
Raffaella Sibillo ◽  
Rosario Cuomo ◽  
Gerardo Nardone ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Chronic Hepatitis ◽  
Quantitative Analysis ◽  
Hepatitis C ◽  
Liver Tissue ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Chronic Liver Diseases ◽  
Liver Biopsies ◽  
Aldolase A

Abstract Background: Chronic liver diseases can progress to cirrhosis and to hepatocellular carcinoma. Timely and unequivocal recognition of the neoplastic evolution of cirrhosis is critical. To this aim, we used a noncompetitive reverse transcription-PCR procedure to analyze aldolase A mRNA in liver tissue from patients with chronic liver diseases at different stages. Methods: We studied 12 patients with hepatocellular carcinoma, 19 patients affected by chronic hepatitis C or cirrhosis, and 7 healthy controls. Aldolase A mRNA was reverse-transcribed to cDNA, which was then amplified by PCR. The amplified segments were “read” with a novel dot-blot procedure. A calibrator with the same sequence, synthesized in vitro using a T7 phage promoter, was processed at scalar dilutions in parallel to the target samples to generate a calibration curve and so quantify the target mRNA (detection limit, 0.03 amol; linearity spanning five orders of magnitude). Results: Aldolase A mRNA was ∼10-fold higher in liver biopsies from patients with hepatocellular carcinoma vs patients with chronic hepatitis C or cirrhosis, and healthy individuals. Furthermore, aldolase A mRNA concentrations were 1.2- to 21.3-fold higher in 12 liver biopsies compared with the paired surrounding cirrhotic tissue. Conclusions: The quantitative analysis of liver tissue aldolase A mRNA differentiates between nonneoplastic chronic liver diseases and hepatocellular carcinoma, which suggests that it has diagnostic potential.

New MR imaging criteria with a diffusion-weighted sequence for the diagnosis of hepatocellular carcinoma in chronic liver diseases

2011 ◽  
Vol 55 (1) ◽  
pp. 126-132 ◽  
Author(s):  
Gilles Piana ◽  
Ludovic Trinquart ◽  
Nawel Meskine ◽  
Vincent Barrau ◽  
Bernard Van Beers ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Mr Imaging ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Chronic Liver Diseases ◽  
Weighted Sequence ◽  
Diffusion Weighted
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