Localization of the heme-binding protein in the cytoplasm and of a heme-binding protein-like immunoreactive protein in the nucleus of rat liver parenchymal cells: Immunocytochemical evidence of the subcellular distribution corroborated by radioimmunoassay and immunoblotting

Hepatology ◽  
1990 ◽  
Vol 11 (5) ◽  
pp. 859-865 ◽  
Author(s):  
H. Dariush Fahimi ◽  
Alfred Voelkl ◽  
Styliani H. Vincent ◽  
Ursula Muller-Eberhard
Keyword(s):  
Rat Liver ◽  
Subcellular Distribution ◽  
Binding Protein ◽  
Heme Binding ◽  
Liver Parenchymal ◽  
Immunoreactive Protein ◽  
Parenchymal Cells ◽  
Heme Binding Protein

scholarly journals The MF6p/FhHDM-1 Major Antigen Secreted by the Trematode Parasite Fasciola hepatica Is a Heme-binding Protein

2013 ◽  
Vol 289 (3) ◽  
pp. 1441-1456 ◽  
Author(s):  
Victoria Martínez-Sernández ◽  
Mercedes Mezo ◽  
Marta González-Warleta ◽  
María J. Perteguer ◽  
Laura Muiño ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Binding Protein ◽  
Blood Feeding ◽  
Reversed Phase ◽  
Heme Binding ◽  
Free Heme ◽  
Major Antigen ◽  
Parenchymal Cells ◽  
Heme Binding Protein

Blood-feeding parasites have developed biochemical mechanisms to control heme intake and detoxification. Here we show that a major antigen secreted by Fasciola hepatica, previously reported as MF6p, of unknown function (gb|CCA61804.1), and as FhHDM-1, considered to be a helminth defense molecule belonging to the family of cathelicidin-like proteins (gb|ADZ24001.1), is in fact a heme-binding protein. The heme-binding nature of the MF6p/FhHDM-1 protein was revealed in two independent experiments: (i) immunopurification of the secreted protein·heme complexes with mAb MF6 and subsequent analysis by C8 reversed-phase HPLC and MS/MS spectrometry and (ii) analysis of the binding ability of the synthetic protein to hemin in vitro. By immunohistochemistry analysis, we have observed that MF6p/FhHDM-1 is produced by parenchymal cells and transported to other tissues (e.g. vitellaria and testis). Interestingly, MF6p/FhHDM-1 is absent both in the intestinal cells and in the lumen of cecum, but it can be released through the tegumental surface to the external medium, where it binds to free heme molecules regurgitated by the parasite after hemoglobin digestion. Proteins that are close analogs of the Fasciola MF6p/FhHDM-1 are present in other trematodes, including Clonorchis, Opistorchis, Paragonimus, Schistosoma, and Dicrocoelium. Using UV-visible spectroscopy and immunoprecipitation techniques, we observed that synthetic MF6p/FhHDM-1 binds to hemin with 1:1 stoichiometry and an apparent Kd of 1.14 × 10−6m−1. We also demonstrated that formation of synthetic MF6p/FhHDM-1·hemin complexes inhibited hemin degradation by hydrogen peroxide and hemin peroxidase-like activity in vitro. Our results suggest that MF6p/FhHDM-1 may be involved in heme homeostasis in trematodes.

scholarly journals Differential Cellular and Subcellular Localization of Heme-Binding Protein 23/Peroxiredoxin I and Heme Oxygenase-1 in Rat Liver

2003 ◽  
Vol 51 (12) ◽  
pp. 1621-1631 ◽  
Author(s):  
Stephan Immenschuh ◽  
Eveline Baumgart-Vogt ◽  
Melly Tan ◽  
Shin-Ichiro Iwahara ◽  
Giuliano Ramadori ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Heme Oxygenase ◽  
Rat Liver ◽  
Binding Protein ◽  
Heme Oxygenase 1 ◽  
Heme Binding ◽  
Heme Binding Protein ◽  
Peroxiredoxin I

Purification, characterization, and cloning of a heme-binding protein (23 kDa) in rat liver cytosol

Biochemistry ◽  
1995 ◽  
Vol 34 (41) ◽  
pp. 13398-13406 ◽  
Author(s):  
Shin-ichiro Iwahara ◽  
Hiroyuki Satoh ◽  
De-Xiu Song ◽  
James Webb ◽  
Alma L. Burlingame ◽  
...  
Keyword(s):  
Rat Liver ◽  
Binding Protein ◽  
Heme Binding ◽  
Liver Cytosol ◽  
Heme Binding Protein ◽  
Rat Liver Cytosol

scholarly journals Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

1976 ◽  
Vol 251 (10) ◽  
pp. 3014-3020 ◽  
Author(s):  
R F Kletzien ◽  
M W Pariza ◽  
J E Becker ◽  
V R Potter ◽  
F R Butcher
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Acid Transport ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells
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