Molecular biology: The polymerase chain reaction

Head & Neck ◽  
1992 ◽  
Vol 14 (1) ◽  
pp. 62-66
Author(s):  
Norris K. Lee
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Chain Reaction ◽  
Polymerase Chain

An intensive hands-on course designed to teach molecular biology techniques to physiology graduate students

2002 ◽  
Vol 26 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Andrea D. Weston ◽  
Sasha Stasko ◽  
Gerald M. Kidder
Keyword(s):  
Polymerase Chain Reaction ◽  
Graduate Students ◽  
Molecular Biology ◽  
Historical Perspective ◽  
Rna Expression ◽  
Chain Reaction ◽  
Hands On ◽  
Similar Information ◽  
Polymerase Chain ◽  
Intensive Course

To address a growing need to make research trainees in physiology comfortable with the tools of molecular biology, we have developed a laboratory-intensive course designed for graduate students. This course is offered to a small group of students over a three-week period and is organized such that comprehensive background lectures are coupled with extensive hands-on experience. The course is divided into seven modules, each organized by a faculty member who has particular expertise in the area covered by that module. The modules focus on basic methods such as cDNA subcloning, sequencing, gene transfer, polymerase chain reaction, and protein and RNA expression analysis. Each module begins with a lecture that introduces the technique in detail by providing a historical perspective, describing both the uses and limitations of that technique, and comparing the method with others that yield similar information. Most of the lectures are followed by a laboratory session during which students follow protocols that were carefully designed to avoid pitfalls. Throughout these laboratory sessions, students are given an appreciation of the importance of proper technique and accuracy. Communication among the students, faculty, and the assistant coordinator is focused on when and why each procedure would be used, the importance of each step in the procedure, and approaches to troubleshooting. The course ends with an exam that is designed to test the students’ general understanding of each module and their ability to apply the various techniques to physiological questions.

Molecular biology made easy. The polymerase chain reaction

1992 ◽  
Vol 24 (12) ◽  
pp. 913-926 ◽  
Author(s):  
A. M. T. Clarke ◽  
N. P. Mapstone ◽  
P. Quirke
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals ОГЛЯД МЕТОДІВ ТА МОДЕЛЕЙ ПОЛІМЕРАЗНО-ЛАНЦЮГОВОЇ РЕАКЦІЇ

2015 ◽  
Author(s):  
A. S. Sverstyuk ◽  
T. V. Bihunyak ◽  
B. O. Pereviznyk
Keyword(s):  
Mathematical Modeling ◽  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Minimum Time ◽  
Chain Reaction ◽  
Reaction Models ◽  
Polymerase Chain ◽  
Time Required

In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques efficiency.

scholarly journals Amplification Of Bacterial Isolate Sf1 Associated With Sponge Facaplysynopsis sp. From Tongkeina, North Sulawesi

2018 ◽  
Vol 6 (2) ◽  
pp. 77
Author(s):  
Liviani Rangian ◽  
Elvy Like Ginting ◽  
Stenly Wullur ◽  
Erly Kaligis ◽  
Sandra Tilaar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Biology ◽  
Genomic Dna ◽  
Bacterial Isolate ◽  
Chain Reaction ◽  
Pcr Polymerase Chain Reaction ◽  
North Sulawesi ◽  
Polymerase Chain

This study was conducted with the aim of amplifying the isolate of SF1 symbiont sponge Facaplysynopsis sp.  from Tongkeina, North Sulawesi. Samples are obtained and stored in the Lab. Molecular Biology and Marine Pharmacology, FPIK Unsrat. The genomic DNA of the samples was isolated using protocols from the Innu PREP Mini DNA Kit. The DNA of the SF1 symbionary bacteria was amplified by PCR (Polymerase Chain Reaction) using an 8F primer (5'-AGAGTITGATCCTGGCTA-3 ') and 1492 R (5'TACCTTACGACTT-3'). DNA bacteria SF1 successfully amplified marked by the appearance of the band of DNA that looks less clear, with a length of 600 bp.Keywords: Bacterial Isolate, Sponge,  AmplificationABSTRAKPenelitian ini dilakukan dengan tujuan mengamplifikasi isolat bakteri SF1 simbion spons Facaplysynopsis sp dari perairan Tongkeina, Sulawesi Utara. Sampel diperoleh dan tersimpan di Lab. Biologi Molekuler dan Farmasitika Laut, FPIK Unsrat. DNA genom dari sampel diisolasi menggunakan protokol dari Innu PREP DNA Mini Kit. DNA bakteri simbion SF1 diamplifikasi dengan PCR (Polymerase Chain Reaction) dengan menggunakan primer 8F (5’-AGAGTITGATCCTGGCTA-3’) dan 1492 R (5’TACCTTACGACTT-3’). DNA bakteri SF1 berhasil diamplifikasi ditandai dengan munculnya pita DNA yang terlihat kurang jelas, dengan panjang 600 bp.Kata Kunci: Bakteri Simbion, Spons, Amplifikasi

Sign in / Sign up

Export Citation Format

Share Document