Use of oral mucosal cell sheets for accelerated oral surgical wound healing

Head & Neck ◽  
2017 ◽  
Vol 40 (2) ◽  
pp. 394-401 ◽  
Author(s):  
Jong-Lyel Roh ◽  
Jaewang Lee ◽  
Hyejin Jang ◽  
Eun Hye Kim ◽  
Daiha Shin
Keyword(s):  
Wound Healing ◽  
Mucosal Cell ◽  
Surgical Wound ◽  
Cell Sheets ◽  
Oral Mucosal Cell

Promotion of oral surgical wound healing using autologous mucosal cell sheets

Oral Oncology ◽  
2017 ◽  
Vol 69 ◽  
pp. 84-91 ◽  
Author(s):  
Jong-Lyel Roh ◽  
Hyejin Jang ◽  
Jaewang Lee ◽  
Eun Hye Kim ◽  
Daiha Shin
Keyword(s):  
Wound Healing ◽  
Mucosal Cell ◽  
Surgical Wound ◽  
Cell Sheets

Plasticity of oral mucosal cell sheets for accelerated and scarless skin wound healing

Oral Oncology ◽  
2017 ◽  
Vol 75 ◽  
pp. 81-88 ◽  
Author(s):  
Jong-Lyel Roh ◽  
Jaewang Lee ◽  
Eun Hye Kim ◽  
Daiha Shin
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Mucosal Cell ◽  
Cell Sheets ◽  
Skin Wound Healing ◽  
Oral Mucosal Cell

scholarly journals Use of a pre-vascularised oral mucosal cell sheet for promoting cutaneous burn wound healing

Theranostics ◽  
2018 ◽  
Vol 8 (20) ◽  
pp. 5703-5712 ◽  
Author(s):  
Jaewang Lee ◽  
Daiha Shin ◽  
Jong-Lyel Roh
Keyword(s):  
Wound Healing ◽  
Cell Sheet ◽  
Mucosal Cell ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Oral Mucosal Cell

scholarly journals Inhibition of Oral Mucosal Cell Wound Healing by Bisphosphonates

2008 ◽  
Vol 66 (5) ◽  
pp. 839-847 ◽  
Author(s):  
Regina Landesberg ◽  
Matthew Cozin ◽  
Serge Cremers ◽  
Victoria Woo ◽  
Stavroula Kousteni ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mucosal Cell ◽  
Oral Mucosal Cell

scholarly journals Accelerated oral wound healing using a pre-vascularized mucosal cell sheet

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jaewang Lee ◽  
Eun Hye Kim ◽  
Daiha Shin ◽  
Jong-Lyel Roh
Keyword(s):  
Wound Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Cell Sheet ◽  
Mucosal Cell ◽  
Sprague Dawley ◽  
Cell Sheets ◽  
Endothelial Progenitor ◽  
Oral Wound Healing

AbstractCell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.

RNA Sequencing Reveals the Wound Repair Mechanism of Cuyuxunxi Prescription in Surgical Patients with Anal Fistulas

2021 ◽  
Vol 24 ◽  
Author(s):  
Yin Qu ◽  
Zhijun Zhang ◽  
Yafeng Lu ◽  
De Zheng ◽  
Wei Yang
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Rna Sequencing ◽  
Anal Fistula ◽  
Wound Repair ◽  
Inflammatory Responses ◽  
Control Group ◽  
Surgical Wound ◽  
Anal Fistulas ◽  
Skin Development

Background: Anal fistula is one of the most common colorectal and perirectal diseases in the world. Cuyuxunxi (CYXX) prescription is an efficient herbal fumigant used to promote the surgical wound healing of anal fistulas. Objective: This study aimed to explore the underlying molecular mechanism of CYXX prescription on surgical wound healing of anal fistulas. Methods: Ten patients with anal fistula were randomized into a control group or treatment group. The wound surface of patients in the control group was rinsed by normal saline, while that in the treatment group was rinsed by CYXX prescription. The wound tissues of patients with anal fistulas seven days after the surgery were collected for hematoxylin-eosin (HE) staining and RNA sequencing. The expressions of differentially expressed genes (DEGs) were validated by real-time quantitative PCR (RT-qPCR). Results: HE staining showed that CYXX treatment reduced the infiltration of inflammatory cells. A total of 472 DEGs, including 141 up-regulated genes and 331 down-regulated genes, were identified. These genes were significantly related to skin development, xenobiotic stimulus, and inflammation. In addition, the consistency rate of RT-qPCR and sequencing results was 83.33%, which showed a high relative reliability of the sequencing results. Conclusion: CYXX prescription could improve epidermis repair and reduce inflammatory responses.

Evaluation of Surgical Wound Healing in Orthopedic Patients with Impaired Tissue Integrity According to Nursing Outcomes Classification

2018 ◽  
Vol 30 (4) ◽  
pp. 228-233 ◽  
Author(s):  
Luciana Nabinger Menna Barreto ◽  
Marcos Barragan da Silva ◽  
Bruna Engelman ◽  
Manoela Schmarczek Figueiredo ◽  
Alba Luz Rodríguez‐Acelas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Surgical Wound ◽  
Tissue Integrity ◽  
Nursing Outcomes ◽  
Nursing Outcomes Classification ◽  
Orthopedic Patients
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