Isoliquiritigenin as a cause of DNA damage and inhibitor of ataxia-telangiectasia mutated expression leading to G2/M phase arrest and apoptosis in oral squamous cell carcinoma

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E360-E371 ◽  
Author(s):  
Shih-Min Hsia ◽  
Cheng-Chia Yu ◽  
Yin-Hua Shih ◽  
Michael Yuanchien Chen ◽  
Tong-Hong Wang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ataxia Telangiectasia ◽  
Ataxia Telangiectasia Mutated ◽  
Phase Arrest ◽  
M Phase

scholarly journals Podophyllotoxin Isolated from Podophyllum peltatum Induces G2/M Phase Arrest and Mitochondrial-Mediated Apoptosis in Esophageal Squamous Cell Carcinoma Cells

Forests ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 8 ◽  
Author(s):  
Goo Yoon ◽  
Mee-Hyun Lee ◽  
Ah-Won Kwak ◽  
Ha-Na Oh ◽  
Seung-Sik Cho ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Er Stress ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Podophyllum Peltatum ◽  
Phase Arrest ◽  
Anti Cancer ◽  
M Phase ◽  
Stress Dependent

Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers in East Asia and is the seventh leading cause of cancer deaths. Podophyllotoxin (PT), a cyclolignan isolated from podophyllum peltatum, exhibits anti-cancer effects at the cellular level. This study investigated the underlying mechanism of anti-cancer effects induced by PT in ESCC cells. Exposure to increasing concentrations of PT led to a significant decrease in the growth and anchorage-independent colony numbers of ESCC cells. PT showed high anticancer efficacy against a panel of four types of ESCC cells, including KYSE 30, KYSE 70, KYSE 410, KYSE 450, and KYSE 510 by IC50 at values ranges from 0.17 to 0.3 μM. We also found that PT treatment induced G2/M phase arrest in the cell cycle and accumulation of the sub-G1 population, as well as apoptosis. Exposure to PT triggered a significant synthesis of reactive oxygen species (ROS), a loss of mitochondrial membrane potential (MMP), and activation of various caspases. Furthermore, PT increased the levels of phosphorylated c-Jun N-terminal kinase (JNK), p38, and the expression of Endoplasmic reticulum (ER) stress marker proteins via ROS generation. An increase in the level of pro-apoptotic proteins and a reduction in the anti-apoptotic protein level induced ESCC cell death via the loss of MMP. Additionally, the release of cytochrome c into the cytosol with Apaf-1 induced the activation of multi-caspases. In conclusion, our results revealed that PT resulted in apoptosis of ESCC cells by modulating ROS-mediated mitochondrial and ER stress-dependent mechanisms. Therefore, PT is a promising therapeutic candidate as an anti-cancer drug against ESCC for clinical use.

Luteolin Impacts on the DNA Damage Pathway in Oral Squamous Cell Carcinoma

2016 ◽  
Vol 68 (5) ◽  
pp. 838-847 ◽  
Author(s):  
Kellen Cristine Tjioe ◽  
Denise Tostes Oliveira ◽  
Julie Gavard
Keyword(s):  
Dna Damage ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell

scholarly journals Protein Expression of DNA Damage Signaling Molecules in Patients with Oral Squamous Cell Carcinoma

2018 ◽  
Vol 2 (1) ◽  
pp. 41-46
Author(s):  
Jigna S Joshi ◽  
Hemangini H Vora ◽  
Nandita R Ghosh ◽  
Jignesh V Goswami ◽  
Trupti I Trivedi
Keyword(s):  
Dna Damage ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Protein Expression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Signaling Molecules ◽  
Dna Damage Signaling
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