scholarly journals Diagnostic potential of 1-step nucleic acid amplification assay in patients with head and neck squamous cell carcinoma based on CK19 expression in a primary lesion

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E239-E245 ◽  
Author(s):  
Masahiro Suzuki ◽  
Takashi Matsuzuka ◽  
Yuko Hashimoto ◽  
Masakazu Ikeda ◽  
Satoshi Saijo ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Nucleic Acid ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Primary Lesion ◽  
Nucleic Acid Amplification ◽  
Diagnostic Potential ◽  
Ck19 Expression ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Oral Oncology ◽  
2020 ◽  
Vol 102 ◽  
pp. 104553 ◽  
Author(s):  
Lucie Peigné ◽  
Florence Godey ◽  
Mathieu Le Gallo ◽  
François Le Gall ◽  
Alain Fautrel ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Lymph Node Metastasis ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Nucleic Acid Amplification ◽  
Node Metastasis ◽  
One Step

One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma

Oral Oncology ◽  
2012 ◽  
Vol 48 (10) ◽  
pp. 958-963 ◽  
Author(s):  
Hiroyuki Goda ◽  
Koh-ichi Nakashiro ◽  
Ryota Oka ◽  
Hiroshi Tanaka ◽  
Hiroyuki Wakisaka ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Lymph Node Metastasis ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Nucleic Acid Amplification ◽  
Node Metastasis ◽  
One Step

scholarly journals Comparison of Different Methods of RNA Preparation from Peripheral Blood for Nucleic Acid Amplification Assay

2018 ◽  
Vol 36 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Dong Eun Lee ◽  
Hanna Lee ◽  
Seon Duk Lee ◽  
Hyung Soo Han ◽  
Jae Young Choe ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peripheral Blood ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

Non-instrumented nucleic acid amplification assay

2008 ◽  
Author(s):  
Bernhard H. Weigl ◽  
Gonzalo Domingo ◽  
Jay Gerlach ◽  
Dennis Tang ◽  
Darrel Harvey ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes

1994 ◽  
Vol 49 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Bob van Gemen ◽  
Rinie van Beuningen ◽  
Arjan Nabbe ◽  
Dianne van Strijp ◽  
Suzanne Jurriaans ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay ◽  
Hiv 1

Quantitative expression study of four cytokeratins and p63 in squamous cell carcinoma of the tongue: suitability for sentinel node navigation surgery using one-step nucleic acid amplification

2011 ◽  
Vol 64 (10) ◽  
pp. 875-879 ◽  
Author(s):  
Kohichi Yamauchi ◽  
Yasunori Fujioka ◽  
Yasunao Kogashiwa ◽  
Naoyuki Kohno
Keyword(s):  
Squamous Cell Carcinoma ◽  
Nucleic Acid ◽  
Sentinel Node ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Nucleic Acid Amplification ◽  
Navigation Surgery ◽  
Sentinel Node Navigation Surgery ◽  
One Step ◽  
Carcinoma Of The Tongue

AimsSentinel node navigation surgery (SNNS) is currently considered to provide better staging of regional metastasis. For rapid and accurate sentinel lymph node analysis, one-step nucleic acid amplification using cytokeratin 19 (CK19) has been applied, particularly in breast cancer. On the other hand, additional quantitative reverse transcription PCR targets containing cytokeratins have been reported recently in head and neck cancer. In this report, CK19 and p63 were immunohistochemically examined in primary tumours for use as molecular markers and were compared with cytokeratin 903 (CK903), cytokeratin 8/18 (CK8/18) and cytokeratin (AE1/AE3), which are used in diagnostic immunohistochemistry for head and neck squamous cell carcinoma.MethodsThe study reviewed 17 patients with T1/T2, N0 (UICC) oral squamous cell carcinoma of the tongue who were treated surgically at Kyorin University Hospital between 2002 and 2009. The intensity and proportion of tumour cells stained for CK19, CK903, p63, CK8/18 and AE1/AE3 were evaluated.ResultsCK19 and CK8/18 staining in cytoplasm was patchy among carcinoma cells, indicating weak expression. Staining proportion for p63, CK903 and AE1/AE3 was greater than for CK19 and CK8/18, although staining intensity for CK903 was weaker than for p63 and AE1/AE3. The difference in total score between CK19 and CK8/18 staining and p63, CK903 and AE1/AE3 staining was statistically significant (p<0.001). p63 and AE1/AE3 may be better markers than CK903, CK19 and CK8/18.ConclusionsThis suggests that p63 is of clinical utility in SNNS and that CK19 is unsuitable for early tongue carcinoma. Further studies are needed before clinical application of these markers.

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