Cooperation of side population cells with CD133 to enrich cancer stem cells in a laryngeal cancer cell line

Head & Neck ◽  
2013 ◽  
pp. n/a-n/a
Author(s):  
Chun-Ping Wu ◽  
Ming Xie ◽  
Liang Zhou ◽  
Lei Tao ◽  
Ming Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Laryngeal Cancer ◽  
Side Population ◽  
Cancer Cell Line ◽  
Side Population Cells

Side Population Cells of Human B-Cell Non-Hodgkin Lymphoma Cell Lines May Contain Lymphoma-Stem Cells Responsible for Lymphoma Initiation

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3105-3105
Author(s):  
Ha Yeon Lee ◽  
Mi Ran Lee ◽  
Won Seog Kim ◽  
Seok Jin Kim
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
B Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Side Population ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Side Population Cells ◽  
Hoechst 33342

Abstract Abstract 3105 Background: Cancer stem cells are considered to initiate cancer development, and such populations might be related with the resistance to treatment and relapse. There are accumulated data supporting the presence of cancer stem cells in several human cancers such as breast and colon cancers. In hematologic malignancy, the presence of cancer stem cells was supported by the previous studies from leukemia. However, there are few reports regarding the possibility of lymphoma stem cells. Side population cells are defined as cells that efficiently extrude the Hoechst 33342 dye, and hence remain negative for this fluorescent marker in flow cytometric analysis. The isolation of SP cells from total tumor cells has been used as a tool for cancer stem cell research. Methods: We performed the side-population analysis to isolate a stem-like cell population. The side population analysis was based on the modified Hoechst 33342 dye efflux assay. Thus, cells expressing ATP binding cassette (ABC) transporter subfamily G number 2 (ABCG2) on their surface efflux Hoechst 33342 dye. On the other hand, cells without ABCG2 expression cannot efflux dye. According to this different nature, we discriminated side population from non-side population. We isolated side population from three lymphoma cell lines, 1A2 (B-cell lymphoblastic lymphoma cell line), Raji (Burkitt lymphoma cell line), and Toledo (Diffuse large B-cell lymphoma cell line). We compared the SP cells with NSP cells from each cell line via in vitro propagation and colony forming assay. We also evaluated the ability of tumorigenesis in NOD/SCID mice and compared the gene expression via performing microarray. Results: We isolated a subset of cells (side population) from three cell lines. The number of side population was extremely small, so the ratio of SP to NSP was from 0.01% to 0.25% regardless of the type of lymphoma cell lines. The in vitro propagation for 3 weeks showed that the growth rate of the side population was significantly higher than non-side population in three cell lines. The life span of non-side population was limited while side population could continuously proliferate. Thus, non-side population could not be cultured over four weeks, and the proportion of viable cells was higher in the side population (≥95%) than non-side population (≤80%). The RT-PCR and confocal microscopy demonstrated the higher expression of ABCG2 in the side population compared to the non-side population. The inoculation of lymphoma cells (200 – 1000) into NOD/SCID mice showed the tumor formation in mice inoculated with side population cells while there was no tumor in non-side population. The hierarchical clustering of differentially expressed genes showed the different pattern of gene expression between side and non-side population in each cell line. Conclusion: This study suggests that the side population may contain a cell population highly capable of proliferation, and this population may have the characteristics of lymphoma stem-like cells. Disclosures: No relevant conflicts of interest to declare.

miRNAs expressed differently in cancer stem cells and cancer cells of human gastric cancer cell line MKN-45

2012 ◽  
Vol 30 (5) ◽  
pp. 411-418 ◽  
Author(s):  
Azadeh Fahim Golestaneh ◽  
Amir Atashi ◽  
Lida Langroudi ◽  
Abbas Shafiee ◽  
Nasser Ghaemi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Human Gastric Cancer

Characterization of FaDu-R, a radioresistant head and neck cancer cell line, and cancer stem cells

2018 ◽  
Vol 45 (3) ◽  
pp. 566-573 ◽  
Author(s):  
Kwang-Jae Cho ◽  
Eun-Ji Park ◽  
Min-Sik Kim ◽  
Young-Hoon Joo
Keyword(s):  
Stem Cells ◽  
Head And Neck Cancer ◽  
Cancer Stem Cells ◽  
Head And Neck ◽  
Cell Line ◽  
Cancer Cell ◽  
Neck Cancer ◽  
Cancer Cell Line
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