scholarly journals Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus

Head & Neck ◽  
2011 ◽  
Vol 33 (4) ◽  
pp. 504-512 ◽  
Author(s):  
Abigail I. Wald ◽  
Elizabeth E. Hoskins ◽  
Susanne I. Wells ◽  
Robert L. Ferris ◽  
Saleem A. Khan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Neck Cell

scholarly journals Afatinib efficacy against squamous cell carcinoma of the head and neck cell lines in vitro and in vivo

2015 ◽  
Vol 10 (4) ◽  
pp. 501-508 ◽  
Author(s):  
Natalie R. Young ◽  
Christian Soneru ◽  
Jing Liu ◽  
Tatyana A. Grushko ◽  
Ashley Hardeman ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Neck Cell

scholarly journals Involvement of EGFR in the response of squamous cell carcinoma of the head and neck cell lines to gefitinib

2008 ◽  
Author(s):  
Takahide Taguchi ◽  
Mamoru Tsukuda ◽  
Yukari Imagawa-Ishiguro ◽  
Yasumasa Kato ◽  
Daisuke Sano
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Neck Cell

scholarly journals Metabolic Plasticity and Combinatorial Radiosensitisation Strategies in Human Papillomavirus-Positive Squamous Cell Carcinoma of the Head and Neck Cell Lines

Cancers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4836
Author(s):  
Mark D. Wilkie ◽  
Emad A. Anaam ◽  
Andrew S. Lau ◽  
Carlos P. Rubbi ◽  
Nikolina Vlatkovic ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Metabolic Profile ◽  
Metabolic Phenotype ◽  
The Impact

Background: A major objective in the management of human papillomavirus (HPV)-positive squamous cell carcinoma of the head and neck (SCCHN) is to reduce long-term functional ramifications while maintaining oncological outcomes. This study examined the metabolic profile of HPV-positive SCCHN and the potential role of anti-metabolic therapeutics to achieve radiosensitisation as a potential means to de-escalate radiation therapy. Methods: Three established HPV-positive SCCHN cell lines were studied (UM-SCC-104, UPCI:SCC154, and VU-SCC-147), together with a typical TP53 mutant HPV-negative SCCHN cell line (UM-SCC-81B) for comparison. Metabolic profiling was performed using extracellular flux analysis during specifically designed mitochondrial and glycolytic stress tests. Sensitivity to ionising radiation (IR) was evaluated using clonogenic assays following no treatment, or treatment with: 25 mM 2-deoxy-D-glucose (glycolytic inhibitor) alone; 20 mM metformin (electron transport chain inhibitor) alone; or 25 mM 2-deoxy-D-glucose and 20 mM metformin combined. Expression levels of p53 and reporters of p53 function (MDM2, p53, Phospho-p53 [Ser15], TIGAR and p21 [CDKN1A]) were examined by western blotting. Results: HPV-positive SCCHN cell lines exhibited a diverse metabolic phenotype, displaying robust mitochondrial and glycolytic reserve capacities. This metabolic profile, in turn, correlated with IR response following administration of anti-metabolic agents, in that both 2-deoxy-D-glucose and metformin were required to significantly potentiate the effects of IR in these cell lines. Conclusions: In contrast to our recently published data on HPV-negative SCCHN cells, which display relative glycolytic dependence, HPV-positive SCCHN cells can only be sensitised to IR using a complex anti-metabolic approach targeting both mitochondrial respiration and glycolysis, reflecting their metabolically diverse phenotype. Notionally, this may provide an attractive platform for treatment de-intensification in the clinical setting by facilitating IR dose reduction to minimise the impact of treatment on long-term function.

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