scholarly journals Magnetic resonance imaging as a tool for in vivo and ex vivo anatomical phenotyping in experimental genetic models

2007 ◽  
Vol 28 (6) ◽  
pp. 555-566 ◽  
Author(s):  
Alain Pitiot ◽  
Zdenka Pausova ◽  
Malcolm Prior ◽  
Jennifer Perrin ◽  
Naomi Loyse ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Ex Vivo ◽  
Genetic Models ◽  
Resonance Imaging

scholarly journals Ex vivo and in vivo magnetic resonance imaging of cartilage canals in swine at predilection sites of osteochondrosis

2013 ◽  
Vol 21 ◽  
pp. S109-S110
Author(s):  
F. Toth ◽  
M.J. Nissi ◽  
J. Zhang ◽  
S. Schmitter ◽  
M. Benson ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Ex Vivo ◽  
Resonance Imaging ◽  
Cartilage Canals

In Vivo and ex Vivo Magnetic Resonance Imaging Evaluation of Early Disc Degeneration with Histopathologic Correlation

Spine ◽  
1991 ◽  
Vol 16 (6) ◽  
pp. 635-640 ◽  
Author(s):  
MARK L. SCHIEBLER ◽  
VICKI J. CAMERINO ◽  
MICHAEL D. FALLON ◽  
MICHAEL B. ZLATKIN ◽  
NICHOLAS GRENIER ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Disc Degeneration ◽  
Ex Vivo ◽  
Resonance Imaging ◽  
Imaging Evaluation ◽  
Magnetic Resonance Imaging Evaluation

scholarly journals Imaging Multiple Sclerosis Pathology at 160μm Isotropic Resolution by Human Whole-Brain Ex Vivo Magnetic Resonance Imaging at 3T

2021 ◽  
Author(s):  
Weigel Matthias ◽  
Dechent Peter ◽  
Galbusera Riccardo ◽  
Bahn Erik ◽  
Nair Govind ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Magnetic Resonance ◽  
Human Brain ◽  
Ex Vivo ◽  
Molecular Layer ◽  
Cortical Lesion ◽  
Resonance Imaging ◽  
Whole Brain

AbstractPostmortem magnetic resonance imaging (MRI) of the fixed healthy and diseased human brain facilitates spatial resolutions and image quality that is not achievable with in vivo MRI scans. Though challenging - and almost exclusively performed at 7T field strength - depicting the tissue architecture of the entire brain in fine detail is invaluable since it enables the study of neuroanatomy and uncovers important pathological features in neurological disorders. The objectives of the present work were (i) to develop a 3D isotropic ultra-high-resolution imaging approach for human whole-brain ex vivo acquisitions working on a standard clinical 3T MRI system, and (ii) to explore the sensitivity and specificity of this concept for specific pathoanatomical features of multiple sclerosis. The reconstructed images demonstrate unprecedented resolution and soft tissue contrast of the diseased human brain at 3T, thus allowing visualization of sub-millimetric lesions in the different cortical layers and in the cerebellar cortex, as well as unique cortical lesion characteristics such as the presence of incomplete / complete iron rims, and patterns of iron accumulation. Further details such as the subpial molecular layer, the line of Gennari, and some intrathalamic nuclei are also well distinguishable.

scholarly journals Subcortical Atlas of the Rhesus Macaque (SARM) for Magnetic Resonance Imaging

2020 ◽  
Author(s):  
Renée Hartig ◽  
Daniel Glen ◽  
Benjamin Jung ◽  
Nikos K. Logothetis ◽  
George Paxinos ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Rhesus Macaque ◽  
Ex Vivo ◽  
Brain Structures ◽  
List Type ◽  
Resonance Imaging ◽  
Magnetic Resonance Imaging Mri ◽  
Dorsal Lateral Geniculate

AbstractDigitized neuroanatomical atlases are crucial for localizing brain structures and analyzing functional networks identified by magnetic resonance imaging (MRI). To aid in MRI data analysis, we have created a comprehensive parcellation of the rhesus macaque subcortex using a high-resolution ex vivo structural imaging scan. The structural scan and its parcellation were warped to the updated NIMH Macaque Template (NMT v2), an in vivo population template, where the parcellation was refined to produce the Subcortical Atlas of the Rhesus Macaque (SARM). The subcortical parcellation and nomenclature reflect those of the 4th edition of the Rhesus Monkey Brain in Stereotaxic Coordinates (RMBSC4; Paxinos et al., in preparation). The SARM features six parcellation levels, arranged hierarchically from fine regions-of-interest (ROIs) to broader composite regions, suited for fMRI studies. As a test, we ran a functional localizer for the dorsal lateral geniculate (DLG) nucleus in three macaques and found significant fMRI activation in this atlas region. The SARM has been made openly available to the neuroimaging community and can easily be used with common MR data processing software, such as AFNI, where the atlas can be embedded into the software alongside cortical macaque atlases.HighlightsWe present the Subcortical Atlas of the Rhesus Macaque (SARM).SARM provides a neuroanatomical reference frame for neuroimaging analysis.The entire subcortex is mapped, including the thalamus, basal ganglia, and brainstem.ROIs are grouped hierarchically, making SARM useful at multiple spatial resolutions.SARM is in the NMT v2 template space and complements the CHARM atlas for the cortex.

Magnetic Resonance Imaging as a Noninvasive Method for Longitudinal Monitoring of Infusion Site Reactions Following Administration of a Novel Apomorphine Formulation

2017 ◽  
Vol 45 (4) ◽  
pp. 472-480 ◽  
Author(s):  
Ronit Shaltiel-Karyo ◽  
Yonit Tsarfati ◽  
Anna Rubinski ◽  
Eduardo Zawoznik ◽  
Irena Weinstock ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Ex Vivo ◽  
Infusion Site ◽  
Resonance Imaging ◽  
Inflammatory Reactions ◽  
Local Site ◽  
Safety Studies ◽  
Magnetic Resonance Imaging Mri

Infusion site reactions are common following subcutaneous infusion of drugs. Such reactions can lead to discontinuation of the treatment. Therefore, assessment of such reactions is essential during preclinical safety studies, and magnetic resonance imaging (MRI) can assist in evaluation. Here, in vivo and ex vivo MRI evaluations were used in addition to classical histopathology to assess the infusion site reaction to ND0701, a novel formulation of apomorphine base developed for the treatment of Parkinson’s disease, in comparison to the commercial apomorphine hydrochloride (HCl) formulation. Both formulations, each at two concentrations, were continuously administered subcutaneously for 20 hr to each of 3 male and 3 female domestic pigs. Based on MRI evaluations, there was a gradual decrease in the volume of the subcutaneous lesions over 4 weeks, with smaller lesions and quicker resolution with ND0701 at concentrations 2.5- to 5-fold higher when compared to the commercial apomorphine HCl formulation. Histopathological evaluation of ND0701 revealed only minimal inflammation at the sites of infusion, whereas the commercial apomorphine HCl caused persistent inflammatory reactions and necrosis. This study provides support to the use of MRI in preclinical testing of subcutaneous drugs when evaluating local site reactions.

scholarly journals Comparison of In Vivo and Ex Vivo Magnetic Resonance Imaging in a Rat Model for Glioblastoma-Associated Epilepsy

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1311
Author(s):  
Charlotte Bouckaert ◽  
Emma Christiaen ◽  
Jeroen Verhoeven ◽  
Benedicte Descamps ◽  
Valerie De Meulenaere ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Ex Vivo ◽  
Mean Diffusivity ◽  
Common Symptom ◽  
Peritumoral Edema ◽  
Resonance Imaging ◽  
Tumor Mass ◽  
Preclinical Treatment

Magnetic resonance imaging (MRI) is frequently used for preclinical treatment monitoring in glioblastoma (GB). Discriminating between tumors and tumor-associated changes is challenging on in vivo MRI. In this study, we compared in vivo MRI scans with ex vivo MRI and histology to estimate more precisely the abnormal mass on in vivo MRI. Epileptic seizures are a common symptom in GB. Therefore, we used a recently developed GB-associated epilepsy model from our group with the aim of further characterizing the model and making it useful for dedicated epilepsy research. Ten days after GB inoculation in rat entorhinal cortices, in vivo MRI (T2w and mean diffusivity (MD)), ex vivo MRI (T2w) and histology were performed, and tumor volumes were determined on the different modalities. The estimated abnormal mass on ex vivo T2w images was significantly smaller compared to in vivo T2w images, but was more comparable to histological tumor volumes, and might be used to estimate end-stage tumor volumes. In vivo MD images displayed tumors as an outer rim of hyperintense signal with a core of hypointense signal, probably reflecting peritumoral edema and tumor mass, respectively, and might be used in the future to distinguish the tumor mass from peritumoral edema—associated with reactive astrocytes and activated microglia, as indicated by an increased expression of immunohistochemical markers—in preclinical models. In conclusion, this study shows that combining imaging techniques using different structural scales can improve our understanding of the pathophysiology in GB.

scholarly journals Imaging multiple sclerosis pathology at 160 μm isotropic resolution by human whole-brain ex vivo magnetic resonance imaging at 3 T

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Matthias Weigel ◽  
Peter Dechent ◽  
Riccardo Galbusera ◽  
Erik Bahn ◽  
Govind Nair ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Magnetic Resonance ◽  
Human Brain ◽  
Ex Vivo ◽  
Molecular Layer ◽  
Cortical Lesion ◽  
Resonance Imaging ◽  
Whole Brain

AbstractPostmortem magnetic resonance imaging (MRI) of the fixed healthy and diseased human brain facilitates spatial resolutions and image quality that is not achievable with in vivo MRI scans. Though challenging—and almost exclusively performed at 7 T field strength—depicting the tissue architecture of the entire brain in fine detail is invaluable since it enables the study of neuroanatomy and uncovers important pathological features in neurological disorders. The objectives of the present work were (1) to develop a 3D isotropic ultra-high-resolution imaging approach for human whole-brain ex vivo acquisitions working on a standard clinical 3 T MRI system; and (2) to explore the sensitivity and specificity of this concept for specific pathoanatomical features of multiple sclerosis. The reconstructed images demonstrate unprecedented resolution and soft tissue contrast of the diseased human brain at 3 T, thus allowing visualization of sub-millimetric lesions in the different cortical layers and in the cerebellar cortex, as well as unique cortical lesion characteristics such as the presence of incomplete/complete iron rims, and patterns of iron accumulation. Further details such as the subpial molecular layer, the line of Gennari, and some intrathalamic nuclei are also well distinguishable.

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