scholarly journals Super‐resolution shadow imaging reveals local remodeling of astrocytic microstructures and brain extracellular space after osmotic challenge

Glia ◽  
2021 ◽  
Author(s):  
Misa Arizono ◽  
V.V.G. Krishna Inavalli ◽  
Stéphane Bancelin ◽  
Mónica Fernández‐Monreal ◽  
U. Valentin Nägerl
Keyword(s):  
Extracellular Space ◽  
Super Resolution ◽  
Shadow Imaging ◽  
Osmotic Challenge ◽  
Brain Extracellular Space

scholarly journals Super-resolution shadow imaging reveals local remodeling of astrocytic microstructures and brain extracellular space after osmotic challenge

2021 ◽  
Author(s):  
Misa Arizono ◽  
V.V.G. Krishna Inavalli ◽  
U. Valentin Nägerl
Keyword(s):  
Brain Tissue ◽  
Extracellular Space ◽  
Temporal Dynamics ◽  
Super Resolution ◽  
Physical Contact ◽  
Cellular Structures ◽  
Technical Limitation ◽  
Brain Physiology ◽  
Shadow Imaging ◽  
Osmotic Challenge

The extracellular space (ECS) plays a central role for brain physiology, shaping the time course and spread of neurochemicals, ions and nutrients that ensure proper brain homeostasis and neuronal communication. Astrocytes are the most abundant type of glia cell in the brain, whose processes densely infiltrate the brain’s parenchyma. As astrocytes are highly sensitive to changes in osmotic pressure, they are capable of exerting a potent physiological influence on the ECS.However, little is known about the spatial distribution and temporal dynamics of the ECS that surrounds astrocytes, owing mostly to a lack of appropriate techniques to visualize the ECS in live brain tissue. Mitigating this technical limitation, we applied the recent SUper-resolution SHadow Imaging technique (SUSHI) to astrocyte-labeled organotypic hippocampal brain slices, which allowed us to concurrently image the complex morphology of astrocytes and the ECS with nanoscale resolution in a live experimental setting.Focusing on ring-like astrocytic microstructures in the spongiform domain, we found them to enclose sizable pools of interstitial fluid and cellular structures like dendritic spines. Upon an experimental osmotic challenge, these microstructures remodeled and swelled up at the expense of the pools, effectively increasing the physical contact between astrocytic and cellular structures.Our study reveals novel facets of the dynamic microanatomical relationships between astrocytes, neuropil and the ECS in living brain tissue, which could be of functional relevance for neuronglia communication in a variety of (patho)physiological settings, e.g. LTP induction, epileptic seizures or acute ischemic stroke, where osmotic disturbances are known to occur.

scholarly journals Super-Resolution Imaging of the Brain Extracellular Space Deep within Intact Live Tissue using Carbon Nanotubes

2019 ◽  
Vol 116 (3) ◽  
pp. 304a-305a
Author(s):  
Antoine G. Godin ◽  
Noémie Danné ◽  
Juan A. Varela ◽  
Gao Zhenghong ◽  
Brahim Lounis ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Extracellular Space ◽  
Super Resolution ◽  
Live Tissue ◽  
Resolution Imaging ◽  
The Brain ◽  
Brain Extracellular Space

scholarly journals Chemical Gradients within Brain Extracellular Space Measured using Low Flow Push–Pull Perfusion Sampling in Vivo

2012 ◽  
Vol 4 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Thomas R. Slaney ◽  
Omar S. Mabrouk ◽  
Kirsten A. Porter-Stransky ◽  
Brandon J. Aragona ◽  
Robert T. Kennedy
Keyword(s):  
Extracellular Space ◽  
Low Flow ◽  
Chemical Gradients ◽  
Brain Extracellular Space

scholarly journals Nanoscale exploration of the extracellular space in the live brain by combining single carbon nanotube tracking and super-resolution imaging analysis

Methods ◽  
2020 ◽  
Vol 174 ◽  
pp. 91-99 ◽  
Author(s):  
Chiara Paviolo ◽  
Federico N. Soria ◽  
Joana S. Ferreira ◽  
Antony Lee ◽  
Laurent Groc ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Extracellular Space ◽  
Super Resolution ◽  
Imaging Analysis ◽  
Resolution Imaging
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