ErbB4 point mutation in CU3 inbred rats affects gonadotropin-releasing-hormone neuronal function via compromised neuregulin-stimulated prostaglandin E2 release from astrocytes

Glia ◽  
2018 ◽  
Vol 67 (2) ◽  
pp. 309-320 ◽  
Author(s):  
Hanna Moeller-Gnangra ◽  
Johanna Ernst ◽  
Manuel Pfeifer ◽  
Sabine Heger
Keyword(s):  
Prostaglandin E2 ◽  
Point Mutation ◽  
Gonadotropin Releasing Hormone ◽  
Neuronal Function ◽  
Releasing Hormone ◽  
Inbred Rats

scholarly journals Essential Role of the Homeodomain for Pituitary Homeobox 1 Activation of Mouse Gonadotropin-Releasing Hormone Receptor Gene Expression through Interactions with c-Jun and DNA

2004 ◽  
Vol 24 (14) ◽  
pp. 6127-6139 ◽  
Author(s):  
Kyeong-Hoon Jeong ◽  
William W. Chin ◽  
Ursula B. Kaiser
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Point Mutation ◽  
Protein Interaction ◽  
Hormone Receptor ◽  
Gene Promoter ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Protein Protein Interaction ◽  
Gonadotropin Releasing Hormone Receptor

ABSTRACT The gonadotropin-releasing hormone receptor (GnRHR) is expressed primarily in the gonadotropes of the anterior pituitary. Pituitary homeobox 1 (Pitx-1) has been shown to activate pituitary-specific gene expression by direct DNA binding and/or protein-protein interaction with other transcription factors. We hypothesized that Pitx-1 might also dictate tissue-specific expression of the mouse GnRHR (mGnRHR) gene in a similar manner. Pitx-1 activated the mGnRHR gene promoter, and transactivation was localized to sequences between −308 and −264. Pitx-1 bound to this region only with low affinity. This region includes an activating protein 1 (AP-1) site, which was previously shown to be important for mGnRHR gene expression. Further characterization indicated that an intact AP-1 site was required for full Pitx-1 responsiveness. Furthermore, Pitx-1 and AP-1 were synergistic in the activation of the mGnRHR gene promoter. A Pitx-1 homeodomain (HD) point mutation, which eliminated DNA binding ability, caused only a partial reduction of transactivation, whereas deletion of the HD completely prevented transactivation. Pitx-1 interacted directly with c-Jun, and the HD was sufficient for this interaction. While the point mutation in the Pitx-1 HD did not affect interaction with c-Jun, deletion of the HD eliminated the interaction. Taken together, our studies indicate that Pitx-1 can direct transactivation of the mGnRHR gene, in part by DNA binding and in part by an action of Pitx-1 as a cofactor for AP-1, augmenting AP-1 activity through a novel protein-protein interaction between c-Jun and the HD of Pitx-1.

186: Neoadjuvant Gonadotropin-Releasing Hormone Therapy Before Orchiopexy Improves the Fertility Index in Concomitant Testicular Biopsies

2004 ◽  
Vol 171 (4S) ◽  
pp. 49-49
Author(s):  
Christian Schwentner ◽  
Andreas Lunacek ◽  
Josef Oswald ◽  
Georg Bartsch ◽  
Alfons Kreczy ◽  
...  
Keyword(s):  
Hormone Therapy ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Fertility Index
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