Astrocytic calcium signals induced by neuromodulators via functional metabotropic receptors in the ventral respiratory group of neonatal mice

Kai Härtel; Christian Schnell; Swen Hülsmann

doi:10.1002/glia.20808

Astrocytic calcium signals induced by neuromodulators via functional metabotropic receptors in the ventral respiratory group of neonatal mice

Glia ◽

10.1002/glia.20808 ◽

2009 ◽

Vol 57 (8) ◽

pp. 815-827 ◽

Cited By ~ 16

Author(s):

Kai Härtel ◽

Christian Schnell ◽

Swen Hülsmann

Keyword(s):

Metabotropic Receptors ◽

Calcium Signals ◽

Ventral Respiratory Group ◽

Neonatal Mice

Download Full-text

Freeze-frame imaging of dendritic calcium signals with TubuTag

10.1101/2020.11.30.403311 ◽

2020 ◽

Author(s):

Alberto Perez-Alvarez ◽

Florian Huhn ◽

Céline D. Dürst ◽

Andreas Franzelin ◽

Paul Lamothe-Molina ◽

...

Keyword(s):

Pyramidal Neurons ◽

Dendritic Tree ◽

Large Field ◽

Metabotropic Receptors ◽

Calcium Signals ◽

Two Photon ◽

Rich Diversity ◽

Large Populations ◽

Subcellular Resolution ◽

Large Neurons

AbstractThe extensive dendritic arbor of neurons is thought to be actively involved in the processing of information. Dendrites contain a rich diversity of ligand- and voltage-activated ion channels as well as metabotropic receptors. In addition, they are capable of releasing calcium from intracellular stores. Under specific conditions, large neurons produce calcium spikes that are locally restricted to a dendritic section. To investigate calcium signaling in dendrites, we introduce TubuTag, a genetically encoded calcium sensor anchored to the cytoskeleton. TubuTag integrates cytoplasmic calcium signals by irreversible photoconversion from green to red fluorescence when illuminated with violet light. To image the mm-long dendritic tree of pyramidal neurons at subcellular resolution, we used a custom two-photon microscope with a large field of view. As the read-out of fluorescence can be performed several hours after photoconversion, TubuTag will help investigating dendritic signal integration and calcium homeostasis in large populations of neurons.

Download Full-text

Freeze-Frame Imaging of Dendritic Calcium Signals With TubuTag

Frontiers in Molecular Neuroscience ◽

10.3389/fnmol.2021.635820 ◽

2021 ◽

Vol 14 ◽

Author(s):

Alberto Perez-Alvarez ◽

Florian Huhn ◽

Céline D. Dürst ◽

Andreas Franzelin ◽

Paul J. Lamothe-Molina ◽

...

Keyword(s):

Pyramidal Neurons ◽

Apical Dendrite ◽

Large Field ◽

Metabotropic Receptors ◽

Calcium Signals ◽

Two Photon ◽

Rich Diversity ◽

Large Populations ◽

Subcellular Resolution ◽

Large Neurons

The extensive dendritic arbor of neurons is thought to be actively involved in the processing of information. Dendrites contain a rich diversity of ligand- and voltage-activated ion channels as well as metabotropic receptors. In addition, they are capable of releasing calcium from intracellular stores. Under specific conditions, large neurons produce calcium spikes that are locally restricted to a dendritic section. To investigate calcium signaling in dendrites, we introduce TubuTag, a genetically encoded ratiometric calcium sensor anchored to the cytoskeleton. TubuTag integrates cytoplasmic calcium signals by irreversible photoconversion from green to red fluorescence when illuminated with violet light. We used a custom two-photon microscope with a large field of view to image pyramidal neurons in CA1 at subcellular resolution. Photoconversion was strongest in the most distal parts of the apical dendrite, suggesting a gradient in the amplitude of dendritic calcium signals. As the read-out of fluorescence can be performed several hours after photoconversion, TubuTag will help investigating dendritic signal integration and calcium homeostasis in large populations of neurons.

Download Full-text