Behavior of in vitro cultured ameboid microglial cells migrating on Müller cell end-feet in the quail embryo retina

Glia ◽  
2006 ◽  
Vol 54 (5) ◽  
pp. 376-393 ◽  
Author(s):  
Mohamed Tassi ◽  
Ruth Calvente ◽  
José L. Marín-Teva ◽  
Miguel A. Cuadros ◽  
Ana M. Santos ◽  
...  
Keyword(s):  
Müller Cell ◽  
Microglial Cells ◽  
Quail Embryo ◽  
Muller Cell

670nm light treatment following retinal injury modulates Müller cell gliosis: Evidence from in vivo and in vitro stress models

2018 ◽  
Vol 169 ◽  
pp. 1-12 ◽  
Author(s):  
Yen-Zhen Lu ◽  
Nilisha Fernando ◽  
Riccardo Natoli ◽  
Michele Madigan ◽  
Krisztina Valter
Keyword(s):  
Light Treatment ◽  
Müller Cell ◽  
Retinal Injury ◽  
Muller Cell ◽  
Stress Models ◽  
In Vitro Stress

Late proliferation of retinal Müller cell progenitors facilitates preferential targeting with retroviral vectors in vitro

1997 ◽  
Vol 20 (3) ◽  
pp. 186-196 ◽  
Author(s):  
Paul J. Linser ◽  
Burkhard Schlosshauer ◽  
Deni S. Galileo ◽  
William R. Buzzi ◽  
Rana C. Lewis
Keyword(s):  
Retroviral Vectors ◽  
Müller Cell ◽  
Muller Cell

Expression of a novel Muller glia specific antigen during development and after optic nerve lesion

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 789-799 ◽  
Author(s):  
B. Schlosshauer ◽  
D. Grauer ◽  
D. Dutting ◽  
J. Vanselow
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Differentiation ◽  
Optic Nerve ◽  
Glial Cell ◽  
Glial Cells ◽  
Ganglion Cells ◽  
Müller Cell ◽  
Cell Type ◽  
Muller Cell

To generate monoclonal antibodies, immunogen fractions were purified from embryonic chick retinae by temperature-induced detergent-phase separation employing Triton X-114. Under reducing conditions, the monoclonal antibody (mAb) 2M6 identifies a protein doublet at 40 and 46 × 10(3) Mr, which appears to form disulfide-coupled multimers. The 2M6 antigen is regulated developmentally during retinal histogenesis and its expression correlates with Muller glial cell differentiation. Isolated glial endfeet and retinal glial cells in vitro were found to be 2M6-positive, identified with the aid of the general glia marker mAb R5. mAb 2M6 does not bind to any other glial cell type in the CNS as judged from immunohistochemical data. Cell-type specificity was further substantiated by employing retinal explant and single cell cultures on laminin in conjunction with two novel neuron-specific monoclonal antibodies. MAb 2M6 does not bind either to neurites or to neuronal cell bodies. Incubation of retinal cells in vitro with bromodeoxyuridine (BrdU) and subsequent immunodouble labelling with mAb 2M6 and anti-BrdU reveal that mitotic Muller cells can also express the 2M6 antigen. To investigate whether Muller cell differentiation depends on interactions with earlier differentiating ganglion cells, transections of early embryonic optic nerves in vivo were performed. This operation eliminates ganglion cells. Muller cell development and 2M6 antigen expression were not affected, suggesting a ganglion-cell-independent differentiation process. If, however, the optic nerve of juvenile chicken was crushed to induce a transient degeneration/regeneration process in the retina, a significant increase of 2M6 immunoreactivity became evident. These data are in line with the hypothesis that Muller glial cells, in contrast to other distinct glial cell types, might facilitate neural regeneration.

Activation of retinal microglial cells is not associated with Müller cell reactivity in vitrectomized rabbit eyes

2012 ◽  
Vol 91 (1) ◽  
pp. e48-e55 ◽  
Author(s):  
Elke Ulbricht ◽  
Thomas Pannicke ◽  
Susann Uhlmann ◽  
Peter Wiedemann ◽  
Andreas Reichenbach ◽  
...  
Keyword(s):  
Müller Cell ◽  
Microglial Cells ◽  
Cell Reactivity ◽  
Muller Cell

scholarly journals Purinergic regulation of high-glucose-induced caspase-1 activation in the rat retinal Müller cell line rMC-1

2011 ◽  
Vol 301 (5) ◽  
pp. C1213-C1223 ◽  
Author(s):  
Katherine E. Trueblood ◽  
Susanne Mohr ◽  
George R. Dubyak
Keyword(s):  
High Glucose ◽  
Müller Cells ◽  
Müller Cell ◽  
Muller Cells ◽  
Interacting Protein ◽  
Caspase 1 ◽  
Muller Cell ◽  
Stimulation Of

Chronic activation of proinflammatory caspase-1 in the retinas of diabetic animals and patients in vivo and retinal Müller cells in vitro is well documented. In this study we characterized how elevated glucose and extracellular purines contribute to the activation of caspase-1 in a cultured rat Müller cell (rMC-1) model. The ability of high glucose (25 mM, 24 h) to activate caspase-1 was attenuated by either apyrase, which metabolizes extracellular ATP to AMP, or adenosine deaminase (ADA), which metabolizes extracellular adenosine to inosine. This suggested that autocrine stimulation of ATP-sensing P2 receptors and adenosine-sensing P1 receptors may in part mediate the response to high glucose. Exogenous ATP, 5′- N-ethylcarboxamido-adenosine (NECA), a nonselective P1 receptor agonist, or forskolin (FSK) increased caspase-1 activity in rMC-1 cells cultured in control glucose (5 mM) medium. Accumulation of active caspase-1 was also increased by dipyridamole, which suppresses adenosine reuptake. High-glucose stimulation of caspase-1 was attenuated by suramin, a nonselective P2 antagonist, or A2 adenosine receptor antagonists, but not by antagonism of P2X7 ATP-gated ion channel receptors. Although high glucose increased P2X7 mRNA, neither P2X7 protein nor function was detected in rMC-1 cells. The increased caspase-1 activity stimulated by high glucose, FSK, NECA, or ATP was correlated with increased gene expression of caspase-1 and thioredoxin-interacting-protein (TXNIP). These findings support a novel role for autocrine P1 and P2 purinergic receptors coupled to cAMP signaling cascades and transcriptional induction of caspase-1 in mediating the high-glucose-induced activation of caspase-1 and secretion of IL-1β in a cell culture model of nonhematopoietic retinal Müller cells.

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