Survival of neural precursor cells in growth factor-poor environment: Implications for transplantation in chronic disease

Glia ◽  
2006 ◽  
Vol 53 (4) ◽  
pp. 449-455 ◽  
Author(s):  
Ofira Einstein ◽  
Ofra Ben-Menachem-Tzidon ◽  
Rachel Mizrachi-Kol ◽  
Etti Reinhartz ◽  
Nikolaos Grigoriadis ◽  
...  
Keyword(s):  
Chronic Disease ◽  
Growth Factor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

scholarly journals Fibroblast growth factor 2 up regulates telomerase activity in neural precursor cells

Oncogene ◽  
2000 ◽  
Vol 19 (26) ◽  
pp. 2957-2966 ◽  
Author(s):  
Stéphane Haïk ◽  
Laurent R Gauthier ◽  
Christine Granotier ◽  
Jean-Michel Peyrin ◽  
Céline Silva Lages ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Telomerase Activity ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Fibroblast Growth Factor 2 ◽  
Neural Precursor ◽  
Fibroblast Growth

scholarly journals Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Pia S. Jensen ◽  
Lise Lyck ◽  
Pia Jensen ◽  
Jens Zimmer ◽  
Morten Meyer
Keyword(s):  
Growth Factor ◽  
Dopaminergic Neurons ◽  
Neural Tissue ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Large Animal ◽  
Ventral Mesencephalic

The potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like Parkinson’s disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons. One current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-termin vitropropagation of the cells. The domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resemblance of the larger gyrencephalic pig brain to the human brain than the commonly used brains of smaller rodents. In the present study, porcine embryonic (28–30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. For passaging, the tissue spheres were cut into quarters, avoiding mechanical or enzymatic dissociation in order to minimize cellular trauma and preserve intercellular contacts. Spheres were propagated for up to 237 days with analysis of cellular content and differentiation at various time points. Our study provides the first demonstration that porcine ventral mesencephalic precursor cells can be long-term propagated as neural tissue spheres, thereby providing an experimental 3Din vitromodel for studies of neural precursor cells, their niche, and differentiation capacity.

scholarly journals The p107/E2F Pathway Regulates Fibroblast Growth Factor 2 Responsiveness in Neural Precursor Cells

2009 ◽  
Vol 29 (17) ◽  
pp. 4701-4713 ◽  
Author(s):  
Kelly A. McClellan ◽  
Jacqueline L. Vanderluit ◽  
Lisa M. Julian ◽  
Matthew G. Andrusiak ◽  
Delphie Dugal-Tessier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Neural Progenitor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Fibroblast Growth Factor 2 ◽  
Neural Precursor ◽  
Fibroblast Growth

ABSTRACT We have previously shown that p107, a member of the retinoblastoma (Rb) cell cycle regulatory family, has a unique function in regulating the pool of neural precursor cells. As the pool of progenitors is regulated by a limiting supply of trophic factors, we asked if the Rb/E2F pathway may control the size of the progenitor population by regulating the levels of growth factors or their receptors. Here, we demonstrate that fibroblast growth factor 2 (FGF2) is aberrantly upregulated in the brains of animals lacking Rb family proteins and that the gene encoding the FGF2 ligand is directly regulated by p107 and E2F3. Chromatin immunoprecipitation assays demonstrated that E2F3 and p107 occupy E2F consensus sites on the FGF2 promoter in the context of native chromatin. To evaluate the physiological consequence of FGF2 deregulation in both p107 and E2F3 mutants, we measured neural progenitor responsiveness to growth factors. Our results demonstrate that E2F3 and p107 are each mediators of FGF2 growth factor responsiveness in neural progenitor cells. These results support a model whereby p107 regulates the pool of FGF-responsive progenitors by directly regulating FGF2 gene expression in vivo. By identifying novel roles for p107/E2F in regulating genes outside of the classical cell cycle machinery targets, we uncover a new mechanism whereby Rb/E2F mediates proliferation through regulating growth factor responsiveness.

P3-055: Growth factor removal and acidic changes affect the major proteolytic systems in human neural precursor cells (NPCs) and promote tau expression and oligomerization

2015 ◽  
Vol 11 (7S_Part_14) ◽  
pp. P639-P639
Author(s):  
Maria del Carmen Cardenas-Aguayo ◽  
Laura Gomez-Virgilio ◽  
Gustavo Lopez-Toledo ◽  
José Luna-Muñoz ◽  
Marco Antonio Meraz-Rios
Keyword(s):  
Growth Factor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Tau Expression

scholarly journals Midkine, a heparin-binding growth factor, is expressed in neural precursor cells and promotes their growth

2006 ◽  
Vol 99 (6) ◽  
pp. 1470-1479 ◽  
Author(s):  
Peng Zou ◽  
Hisako Muramatsu ◽  
Takaki Miyata ◽  
Takashi Muramatsu
Keyword(s):  
Growth Factor ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Heparin Binding
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