Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line

Glia ◽  
2005 ◽  
Vol 51 (3) ◽  
pp. 187-198 ◽  
Author(s):  
Monica Boveri ◽  
Vincent Berezowski ◽  
Anna Price ◽  
Stephanie Slupek ◽  
Anne-Marie Lenfant ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Glial Cells ◽  
Barrier Properties ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Blood Brain ◽  
Capillary Endothelial Cells ◽  
C6 Cell Line

scholarly journals TNAP and EHD1 Are Over-Expressed in Bovine Brain Capillary Endothelial Cells after the Re-Induction of Blood-Brain Barrier Properties

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e48428 ◽  
Author(s):  
Barbara Deracinois ◽  
Sophie Duban-Deweer ◽  
Gwënaël Pottiez ◽  
Roméo Cecchelli ◽  
Yannis Karamanos ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Barrier Properties ◽  
Bovine Brain ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells

scholarly journals In Vitro Blood-Brain Barrier Models Using Brain Capillary Endothelial Cells Isolated from Neonatal and Adult Rats Retain Age-Related Barrier Properties

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55166 ◽  
Author(s):  
Fuyuko Takata ◽  
Shinya Dohgu ◽  
Atsushi Yamauchi ◽  
Junichi Matsumoto ◽  
Takashi Machida ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Barrier Properties ◽  
Brain Barrier ◽  
Adult Rats ◽  
Brain Capillary ◽  
Blood Brain ◽  
Age Related ◽  
Capillary Endothelial Cells

Receptor-mediated transcytosis of transferrin through blood-brain barrier endothelial cells

1996 ◽  
Vol 270 (4) ◽  
pp. H1149-H1158 ◽  
Author(s):  
L. Descamps ◽  
M. P. Dehouck ◽  
G. Torpier ◽  
R. Cecchelli
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Bovine Brain ◽  
Brain Barrier ◽  
Double Labeling ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells ◽  
A Cell

A cell culture model of the blood-brain barrier consisting of a coculture of bovine brain capillary endothelial cells (BBCECs) and astrocytes has been used to examine the mechanism of iron transport to the brain. Binding experiments showed that BBCECs express 35,000 high-affinity (concn at 50% receptor saturation = 11.3 +/- 2.1 nM) transferin (Tf) receptors per cell. In contrast to apo-transferrin (apoTf) we observed a specific transport of holo-transferrin (holoTf) across BBCECs. This transport was inhibited completely at low temperature. Moreover, the anti-Tf receptor antibody (OX-26) competitively inhibited holoTf uptake by BBCECs. Pulse-chase experiments demonstrated that only 10% of Tf was recycled to the luminal side of the cells, whereas the majority of Tf was transcytosed to the abluminal side; double-labeling experiments clearly demonstrated that iron crosses BBCECs bound to Tf. No intraendothelial degradation of Tf was observed, suggesting that the intraendothelial pathway through BBCECs bypasses the lysosomal compartment. These results clearly show that the iron-Tf complex is transcytosed across brain capillary endothelial cells by a receptor-mediated pathway without any degradation.

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