Transforming growth factor-? and forskolin attenuate the adverse effects of long-term Schwann cell denervation on peripheral nerve regeneration in vivo

Glia ◽  
2002 ◽  
Vol 37 (3) ◽  
pp. 206-218 ◽  
Author(s):  
Olawale A.R. Sulaiman ◽  
Tessa Gordon
Keyword(s):  
Growth Factor ◽  
Adverse Effects ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Transforming Growth Factor ◽  
Peripheral Nerve Regeneration

Resorbable Structures for Schwann Cell Enhanced Peripheral Nerve Regeneration

1998 ◽  
Vol 550 ◽  
Author(s):  
A.E. Silva ◽  
LC. Summerhayes ◽  
D.J. Trantolo ◽  
D.L. Wise ◽  
M.V. Catftaneo ◽  
...  
Keyword(s):  
Growth Factor ◽  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Cell Movement ◽  
Regenerative Process

AbstractSchwann cells play a dual role serving as a physical framework for regenerating nerves, providing extracellular matrix proteins and specific adhesion molecules facilitating attachment and cell movement, and as a source of stimulatory factors mediated by the release or reception of different ligands important in growth and cell signaling events. To investigate the role of one such ligand, glial growth factor (GGF), in peripheral nerve regeneration, a bioabsorbable nerve guide, prepared from a poly(lactic-co-glycolic) acid (PLGA) foam was seeded with autogenous Schwann cells in the presence and absence of growth factor and evaluated in vivo using a rat sciatic nerve regeneration model. Four weeks post-operatively peripheral nerve regeneration was evident. The resorbable foam implant demonstrated extensive neo-vascularization in and around the guide with no evidence of an inflammatory response or encapsulation. The study showed a statistically significant increase in all measured parameters of nerve regeneration in the presence of GGF. Increased numbers of blood vessels in the regenerated tissue accompanied increased total axon counts after twelve weeks. The addition of exogenous Schwann cells resulted in reduced total axon counts perhaps due to the competition for limited growth factors released by the regenerating tissues. The Schwann cell groups, however, displayed the highest myelination indices recorded likely reflecting the role of Schwann cells in the myelination process. Measurements of conduction velocities (EMGs) revealed the highest conductance velocities recorded in nerves regenerated in the presence of both GGF and Schwann cells. Clearly, the inclusion of GGF in the nerve regenerative process is beneficial with respect to both the generation of new axons and the establishment of a functional endpoint.

Acidic Fibroblast Growth Factor Enhances Peripheral Nerve Regeneration in Vivo

1989 ◽  
Vol 83 (6) ◽  
pp. 1020-1021 ◽  
Author(s):  
Peter G. Cordeiro ◽  
Brooke R. Seckel ◽  
Stuart A. Lipton ◽  
Patricia A. DʼAmore ◽  
John Wagner ◽  
...  
Keyword(s):  
Growth Factor ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Fibroblast Growth Factor ◽  
Peripheral Nerve Regeneration ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor

scholarly journals Fibroblasts Colonizing Nerve Conduits Express High Levels of Soluble Neuregulin1, a Factor Promoting Schwann Cell Dedifferentiation

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1366 ◽  
Author(s):  
Benedetta E. Fornasari ◽  
Marwa El Soury ◽  
Giulia Nato ◽  
Alessia Fucini ◽  
Giacomo Carta ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Good Alternative ◽  
Cell Dedifferentiation ◽  
Nerve Conduits ◽  
Early Regeneration

Conduits for the repair of peripheral nerve gaps are a good alternative to autografts as they provide a protected environment and a physical guide for axonal re-growth. Conduits require colonization by cells involved in nerve regeneration (Schwann cells, fibroblasts, endothelial cells, macrophages) while in the autograft many cells are resident and just need to be activated. Since it is known that soluble Neuregulin1 (sNRG1) is released after injury and plays an important role activating Schwann cell dedifferentiation, its expression level was investigated in early regeneration steps (7, 14, 28 days) inside a 10 mm chitosan conduit used to repair median nerve gaps in Wistar rats. In vivo data show that sNRG1, mainly the isoform α, is highly expressed in the conduit, together with a fibroblast marker, while Schwann cell markers, including NRG1 receptors, were not. Primary culture analysis shows that nerve fibroblasts, unlike Schwann cells, express high NRG1α levels, while both express NRG1β. These data suggest that sNRG1 might be mainly expressed by fibroblasts colonizing nerve conduit before Schwann cells. Immunohistochemistry analysis confirmed NRG1 and fibroblast marker co-localization. These results suggest that fibroblasts, releasing sNRG1, might promote Schwann cell dedifferentiation to a “repair” phenotype, contributing to peripheral nerve regeneration.

Improved Peripheral Nerve Regeneration in Streptozotocin-Induced Diabetic Rats by Oral Lumbrokinase

2015 ◽  
Vol 43 (02) ◽  
pp. 215-230 ◽  
Author(s):  
Han-Chung Lee ◽  
Yuan-Man Hsu ◽  
Chin-Chuan Tsai ◽  
Cherng-Jyh Ke ◽  
Chun-Hsu Yao ◽  
...  
Keyword(s):  
Growth Factor ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Transforming Growth Factor ◽  
Peripheral Nerve Regeneration ◽  
Interleukin 1 ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Nerve Lesion

We assessed the therapeutic effects of lumbrokinase, a group of enzymes extracted from the earthworm, on peripheral-nerve regeneration using well-defined sciatic nerve lesion paradigms in diabetic rats induced by the injection of streptozotocin (STZ). We found that lumbrokinase therapy could improve the rats' circulatory blood flow and promote the regeneration of axons in a silicone rubber conduit after nerve transection. Lumbrokinase treatment could also improve the neuromuscular functions with better nerve conductive performances. Immunohistochemical staining showed that lumbrokinase could dramatically promote calcitonin gene-related peptide (CGRP) expression in the lamina I–II regions in the dorsal horn ipsilateral to the injury and cause a marked increase in the number of macrophages recruited within the distal nerve stumps. In addition, the lumbrokinase could stimulate the secretion of interleukin-1 (IL-1), nerve growth factor (NGF), platelet-derived growth factor (PDGF), and transforming growth factor-β (TGF-β) in dissected diabetic sciatic nerve segments. In conclusion, the administration of lumbrokinase after nerve repair surgery in diabetic rats was found to have remarkable effects on promoting peripheral nerve regeneration and functional recovery.

scholarly journals Human Hair Derived Keratins Mediate Schwann Cell Behavior in vitro and Facilitate Rapid Peripheral Nerve Regeneration in vivo

2007 ◽  
Vol 21 (6) ◽  
Author(s):  
Paulina Sierpinski ◽  
Jeffrey Garrett ◽  
Jianjun Ma ◽  
Peter Apel ◽  
Tom Smith ◽  
...  
Keyword(s):  
Schwann Cell ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Human Hair ◽  
Peripheral Nerve Regeneration ◽  
Cell Behavior

Inducible Nerve Growth Factor Delivery for Peripheral Nerve Regeneration In Vivo

2010 ◽  
Vol 126 (6) ◽  
pp. 1874-1889 ◽  
Author(s):  
Thomas Scholz ◽  
Jason M. Rogers ◽  
Alisa Krichevsky ◽  
Sanjay Dhar ◽  
Gregory R. D. Evans
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Growth Factor Delivery ◽  
Nerve Growth

Acidic Fibroblast Growth Factor Enhances Peripheral Nerve Regeneration in Vivo

1989 ◽  
Vol 83 (6) ◽  
pp. 1013-1019 ◽  
Author(s):  
Peter G. Cordeiro ◽  
Brooke R. Seckel ◽  
Stuart A. Lipton ◽  
Patricia A. DʼAmore ◽  
John Wagner ◽  
...  
Keyword(s):  
Growth Factor ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Fibroblast Growth Factor ◽  
Peripheral Nerve Regeneration ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor
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