Expression of the whey acidic protein (Wap) is necessary for adequate nourishment of the offspring but not functional differentiation of mammary epithelial cells

genesis ◽  
2005 ◽  
Vol 43 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Aleata A. Triplett ◽  
Kazuhito Sakamoto ◽  
Laurice A. Matulka ◽  
Liya Shen ◽  
Gilbert H Smith ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Functional Differentiation ◽  
Whey Acidic Protein ◽  
Mammary Epithelial ◽  
Acidic Protein

scholarly journals Regulatory function of whey acidic protein in the proliferation of mouse mammary epithelial cells in vivo and in vitro

2004 ◽  
Vol 274 (1) ◽  
pp. 31-44 ◽  
Author(s):  
Naoko Nukumi ◽  
Kayoko Ikeda ◽  
Megumi Osawa ◽  
Tokuko Iwamori ◽  
Kunihiko Naito ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Whey Acidic Protein ◽  
Mammary Epithelial ◽  
Acidic Protein ◽  
Regulatory Function

Mammary fibroblasts stimulate growth, alveolar morphogenesis, and functional differentiation of normal rat mammary epithelial cells

2000 ◽  
Vol 36 (9) ◽  
pp. 578-592 ◽  
Author(s):  
Kathleen M. Darcy ◽  
Danilo Zangani ◽  
Wendy Shea-Eaton ◽  
Suzanne F. Shoemaker ◽  
Ping-Ping H. Lee ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Functional Differentiation ◽  
Mammary Epithelial ◽  
Normal Rat

Role of extracellular matrix and prolactin in functional differentiation of bovine BME-UV1 mammary epithelial cells

2011 ◽  
Vol 14 (3) ◽  
pp. 433-442 ◽  
Author(s):  
M. Kozłowski ◽  
J. Wilczak ◽  
T. Motyl ◽  
M. Gajewska
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Culture System ◽  
Mammary Epithelial Cells ◽  
3D Culture ◽  
Milk Proteins ◽  
Functional Differentiation ◽  
Mammary Epithelial

Role of extracellular matrix and prolactin in functional differentiation of bovine BME-UV1 mammary epithelial cells Interactions between extracellular matrix (ECM) and epithelial cells are necessary for proper organisation and function of the epithelium. In the present study we show that bovine mammary epithelial cell line BME-UV1 cultured on ECM components, commercially available as Matrigel™, constitutes a good model for studying mechanisms controlling functional differentiation of the bovine mammary gland. In contact with Matrigel BME-UV1 cells induce apicobasal polarity, and within 16 days form three dimensional (3D) acinar structures with a centrally localized hollow lumen, which structurally resemble mammary alveoli present in the functionally active mammary gland. We have shown that the 3D culture system enables a high expression and proper localisation of integrin receptors and tight junction proteins in BME-UV1 cells to be induced. This effect was not obtained in cells grown in the classical 2D culture system on plastic. Moreover, ECM highly stimulated the synthesis of one of the major milk proteins, β-casein, even in the absence of prolactin. Our results show that contact with ECM plays an important role in the lactogenic activity of bovine MECs, however, prolactin is necessary for the efficient secretion of milk proteins.

scholarly journals The microstructure of laminin-111 compensates for dystroglycan loss in functional differentiation of mammary epithelial cells

2019 ◽  
Author(s):  
A Kent ◽  
N Mayer ◽  
JL Inman ◽  
C Hochman-Mendez ◽  
MJ Bissell ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Mammary Epithelial Cells ◽  
Milk Proteins ◽  
Fractal Dimensions ◽  
Functional Differentiation ◽  
Mammary Epithelial ◽  
Cell Surface Receptor ◽  
List Type ◽  
Surface Receptor

Abstract:Laminin-111, an extracellular matrix (ECM) glycoprotein found in the basement membrane of mammary gland epithelia, is essential for lactation. In mammary epithelial cells, dystroglycan (Dg) is believed to be necessary for polymerization of laminin-111 into networks, thus we asked whether correct polymerization could compensate for Dg loss. Artificially polymerized laminin-111 and the laminin-glycoprotein mix Matrigel, both formed branching, spread networks with fractal dimensions from 1.7-1.8, whereas laminin-111 in media formed small aggregates without fractal properties (a fractal dimension of 2). In Dg knockout cells, either polymerized laminin-111 or Matrigel readily attached to the cell surface, whereas aggregated laminin-111 did not. In contrast, polymerized and aggregated laminin-111 bound similarly to Dg knock-ins. Both polymerized laminin-111 and Matrigel promoted cell rounding, clustering, formation of tight junctions, and expression of milk proteins, whereas aggregated Ln-1 did not attach to cells or promote functional differentiation.HighlightsLaminin assembles into a fractal network when in presence of either the cell surface receptor dystroglycan or acidic glycoproteins or an acidic buffer.When this microstructure is recreated with an acidic treatment, laminin binds readily to dystroglycan null cells and induces functional differentiation of mammary epithelial cells.

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