Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line

genesis ◽  
2001 ◽  
Vol 30 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Rainer Constien ◽  
Anne Forde ◽  
Birgit Liliensiek ◽  
Hermann-Josef Gröne ◽  
Peter Nawroth ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Egfp Reporter ◽  
Cre Recombination

scholarly journals Generation and characterization of a Notch1 signaling-specific reporter mouse line

genesis ◽  
2012 ◽  
Vol 50 (9) ◽  
pp. 700-710 ◽  
Author(s):  
Emma Smith ◽  
Stéphanie Claudinot ◽  
Rajwinder Lehal ◽  
Luca Pellegrinet ◽  
Yann Barrandon ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Notch1 Signaling

scholarly journals A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Madina Karimova ◽  
Oliver Baker ◽  
Aylin Camgoz ◽  
Ronald Naumann ◽  
Frank Buchholz ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Cre Recombination

Generation and characterization of Tbx1-AmCyan1 transgenic reporter mouse line that selectively labels developing thymus primordium

2012 ◽  
Vol 22 (3) ◽  
pp. 659-666
Author(s):  
Wataru Kimura ◽  
Mohammad Tofael Kabir Sharkar ◽  
Nishat Sultana ◽  
Mohammod Johirul Islam ◽  
Tadayoshi Uezato ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse

scholarly journals Characterization of the Expression of the ATP-Gated P2X7 Receptor Following Status Epilepticus and during Epilepsy Using a P2X7-EGFP Reporter Mouse

2020 ◽  
Vol 36 (11) ◽  
pp. 1242-1258 ◽  
Author(s):  
James Morgan ◽  
Mariana Alves ◽  
Giorgia Conte ◽  
Aida Menéndez-Méndez ◽  
Laura de Diego-Garcia ◽  
...  
Keyword(s):  
Status Epilepticus ◽  
P2x7 Receptor ◽  
Reporter Mouse ◽  
Egfp Reporter

scholarly journals Characterization of a Dmd EGFP reporter mouse as a tool to investigate dystrophin expression

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mina V. Petkova ◽  
Susanne Morales-Gonzales ◽  
Karima Relizani ◽  
Esther Gill ◽  
Franziska Seifert ◽  
...  
Keyword(s):  
Reporter Mouse ◽  
Dystrophin Expression ◽  
Egfp Reporter

Generation and characterization of the PKCγ-Cre mouse line

genesis ◽  
2005 ◽  
Vol 43 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yu-Qiang Ding ◽  
Chuan-Xi Xiang ◽  
Zhou-Feng Chen
Keyword(s):  
Mouse Line

scholarly journals Immune cell-specific Cre reporter mouse labels a subset of CNS neurons

2019 ◽  
Author(s):  
Aurélie Bouteau ◽  
Botond Z. Igyártó
Keyword(s):  
Immune Cells ◽  
Langerhans Cells ◽  
Immune Cell ◽  
Neuronal Marker ◽  
Reporter Mouse ◽  
Cns Neurons ◽  
The Central Nervous System ◽  
Antigen Presenting

AbstractHuLangerin-Cre-YFPf/f mice were generated to specifically mark a subset of antigen presenting immune cells, called Langerhans cells (LCs). During histological characterization of these mice, we found that, in addition to LCs an uncharacterized cell population in the central nervous system (CNS) also expressed YFP. In this study, we found that the CNS YFP+ cells were negative for microglia and astrocyte markers, but they expressed mature neuronal marker NeuN and showed neuronal localization/morphology. Thus, these mice might be used to study the ontogeny, migration and the role of a subset of CNS neurons.

Generation and characterization of a mouse line carrying Reck-CreERT2 knock-in allele

genesis ◽  
2018 ◽  
Vol 56 (4) ◽  
pp. e23099 ◽  
Author(s):  
Tomoko Matsuzaki ◽  
Huan Wang ◽  
Yukio Imamura ◽  
Shunya Kondo ◽  
Shuichiro Ogawa ◽  
...  
Keyword(s):  
Mouse Line

Abstract 081: Reporter Mouse Strain Provides a Novel Look at Angiotensin Type-2 Receptor Distribution in Central Nervous System Cardiovascular Control Centers

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
Annette D de Kloet ◽  
Lei Wang ◽  
Jacob A Ludin ◽  
Helmut Hiller ◽  
Justin A Smith ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Fluid Balance ◽  
Hypothalamic Nucleus ◽  
Reporter Mouse ◽  
Arterial Blood ◽  
Egfp Reporter ◽  
Angiotensin Type 2 Receptor ◽  
The Brain ◽  
Angiotensin Type

It is established that angiotensin-II acts at its type-1 receptor (AT1R) in the brain to increase sympathetic outflow and blood pressure, and modulate fluid balance. However, the role of the angiotensin type-2 receptor (AT2R) in the neural control of these processes has received far less attention, largely because of an inability to effectively localize these receptors at a cellular level in the brain. The present studies combine the use of a bacterial artificial chromosome transgenic AT2R-eGFP reporter mouse with recent advances in in situ hybridization (ISH) to circumvent this obstacle. Dual IHC/ ISH studies validated the AT2R-eGFP reporter mice by determining that eGFP and AT2R mRNA were highly co-localized within the nucleus of the solitary tract (NTS; 98.0 ± 0.18 %; 125 ± 3.6 of 127 ± 3.9 cells; n = 4). Analysis of eGFP immunoreactivity in the brain revealed localization to neurons within nuclei that regulate blood pressure and fluid balance (e.g., NTS and median preoptic nucleus [MnPO]). Additional IHC/ISH studies uncovered the phenotype of specific AT2R-eGFP cells. For example, within the NTS, AT2R-eGFP neurons primarily express glutamic acid decarboxylase-67 (GABAergic; 80 ± 2.8 %; 225 ± 12.5 of 280 ± 8.4 cells; n = 4), while only a subset express vesicular glutamate transporter-2 (glutamatergic; 18.2 ± 2.9 %; 50.8 ± 7.7 of 280 ± 8.4 cells) or AT1R (8.7 ± 1.0 %; 22 ± 2.2 of 256 ± 11.7 cells). No co-localization was observed with tyrosine hydroxylase in the NTS. Although AT2R-eGFP neurons were not observed within the paraventricular hypothalamic nucleus (PVN), eGFP was localized to efferents terminating in the PVN and to GABAergic neurons surrounding this nucleus. Retrograde neuronal tract tracing studies revealed that many eGFP-positive efferents to the PVN arise from neurons in the MnPO. Based on these neuroanatomical results, we hypothesized that activation of central AT2R would decrease blood pressure. Consistent with this hypothesis, chronic administration of the selective AT2R agonist, compound 21 (7.5 ng/h into the lateral cerebral ventricle) reduced baseline mean arterial blood pressure relative to control mice (103 ± 1.65 v. 110 ± 1.70 mmHg; n = 16; p = 0.02). These studies demonstrate that central AT2R are positioned to regulate blood pressure.

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