Zebrafish intestinal fatty acid binding protein (I-FABP) gene promoter drives gut-specific expression in stable transgenic fish

genesis ◽  
2004 ◽  
Vol 38 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Guor Mour Her ◽  
Chia-Chang Chiang ◽  
Jen-Leih Wu
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Gene Promoter ◽  
Transgenic Fish ◽  
Specific Expression ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Fabp Gene

scholarly journals Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

2008 ◽  
Vol 60 (2) ◽  
pp. 408-413 ◽  
Author(s):  
F.C. Figueiredo ◽  
P.S. Lopes ◽  
A.P.G. Pinto ◽  
D.A.F. Paiva ◽  
P.T. Mendonça ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Genetic Groups ◽  
Pcr Products ◽  
Fabp Gene

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

Differentiation-dependent activation of the extracellular fatty acid binding protein (Ex-FABP) gene during chondrogenesis

2003 ◽  
Vol 198 (1) ◽  
pp. 144-154 ◽  
Author(s):  
Paolo Giannoni ◽  
Adriana Zambotti ◽  
Aldo Pagano ◽  
Ranieri Cancedda ◽  
Beatrice Dozin
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Extracellular Fatty Acid ◽  
Fabp Gene

scholarly journals Light microscopic localization of hepatic fatty acid binding protein mRNA in jejunal epithelia of rats using in situ hybridization, immunohistochemical, and autoradiographic techniques.

1990 ◽  
Vol 38 (1) ◽  
pp. 111-115 ◽  
Author(s):  
S Iseki ◽  
H Kondo
Keyword(s):  
In Situ Hybridization ◽  
Fatty Acid ◽  
Epithelial Cells ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Hepatic Fatty Acid ◽  
Fabp Gene

An in situ hybridization technique using a [35S]-labeled oligonucleotide probe was employed, in combination with immunohistochemistry and autoradiography, to examine gene expression for hepatic fatty acid binding protein (FABP) in the jejunal epithelia from both fed and fasted rats. In rats fed ad libitum, immunoreactivity and mRNA signal for FABP were localized to the absorptive epithelial cells lining the villus, whereas they were absent in the crypt epithelial cells. The level of FABP mRNA was relatively low in the tip of the villus, although FABP immunoreactivity remained high in this area. Animals fasted for 3 days exhibited a downward shift of the lower boundary of the FABP-expressing cell population into the middle portion of the crypt, in terms of the immunoreactivity and the mRNA signal. The proliferative cell compartment of the crypt, as revealed by [3H]-TdR incorporation, showed no substantial change in size between the fed and fasted states. The present results provided evidence that (a) during the differentiation and upward migration of the absorptive epithelial cells, the expression of FABP gene begins at the crypt-villus junction and declines before the cells reach the villus tip, and (b) fasting induces an earlier expression of the FABP gene in the maturing crypt epithelial cells.

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