Thearrest gene is required for germline cyst formation duringDrosophila oogenesis

genesis ◽  
2001 ◽  
Vol 29 (4) ◽  
pp. 196-209 ◽  
Author(s):  
Michael J. Parisi ◽  
Wei Deng ◽  
Zhong Wang ◽  
Haifan Lin
Keyword(s):  
Cyst Formation ◽  
Germline Cyst

scholarly journals Cyclin A Associates with the Fusome during Germline Cyst Formation in the Drosophila Ovary

2000 ◽  
Vol 218 (1) ◽  
pp. 53-63 ◽  
Author(s):  
Mary A. Lilly ◽  
Margaret de Cuevas ◽  
Allan C. Spradling
Keyword(s):  
Cyclin A ◽  
Cyst Formation ◽  
Germline Cyst ◽  
Drosophila Ovary

scholarly journals Altered germline cyst formation and oogenesis in Tex14 mutant mice

Biology Open ◽  
2021 ◽  
Vol 10 (6) ◽  
Author(s):  
Kanako Ikami ◽  
Nafisa Nuzhat ◽  
Haley Abbott ◽  
Ronald Pandoy ◽  
Lauren Haky ◽  
...  
Keyword(s):  
Germ Cells ◽  
Cyst Formation ◽  
Intercellular Bridge ◽  
Wild Type ◽  
Intercellular Bridges ◽  
Mutant Female ◽  
Homozygous Mutant ◽  
Germline Cyst ◽  
Oocyte Differentiation

ABSTRACT During oocyte differentiation in mouse fetal ovaries, sister germ cells are connected by intercellular bridges, forming germline cysts. Within the cyst, primary oocytes form via gaining cytoplasm and organelles from sister germ cells through germ cell connectivity. To uncover the role of intercellular bridges in oocyte differentiation, we analyzed mutant female mice lacking testis-expressed 14 (TEX14), a protein involved in intercellular bridge formation and stabilization. In Tex14 homozygous mutant fetal ovaries, germ cells divide to form a reduced number of cysts in which germ cells remained connected via syncytia or fragmented cell membranes, rather than normal intercellular bridges. Compared with wild-type cysts, homozygous mutant cysts fragmented at a higher frequency and produced a greatly reduced number of primary oocytes with precocious cytoplasmic enrichment and enlarged volume. By contrast, Tex14 heterozygous mutant germline cysts were less fragmented and generate primary oocytes at a reduced size. Moreover, enlarged primary oocytes in homozygous mutants were used more efficiently to sustain folliculogenesis than undersized heterozygous mutant primary oocytes. Our observations directly link the nature of fetal germline cysts to oocyte differentiation and development.

scholarly journals Orbit/CLASP Is Required for Germline Cyst Formation through Its Developmental Control of Fusomes and Ring Canals in Drosophila Males

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58220 ◽  
Author(s):  
Chie Miyauchi ◽  
Daishi Kitazawa ◽  
Itaru Ando ◽  
Daisuke Hayashi ◽  
Yoshihiro H. Inoue
Keyword(s):  
Cyst Formation ◽  
Developmental Control ◽  
Ring Canals ◽  
Germline Cyst

Female mouse germ cells form synchronously dividing cysts

Development ◽  
1998 ◽  
Vol 125 (17) ◽  
pp. 3323-3328 ◽  
Author(s):  
M.E. Pepling ◽  
A.C. Spradling
Keyword(s):  
Confocal Microscopy ◽  
Germ Cells ◽  
Female Mouse ◽  
Progenitor Cell ◽  
Molecular Level ◽  
Cyst Formation ◽  
Intercellular Bridges ◽  
Cell Clusters ◽  
Key Characteristics ◽  
Germline Cyst

Oocytes from many invertebrates initiate development within distinctive cysts of interconnected cells, which are formed through synchronous divisions of a progenitor cell. Recently, processes underlying cyst formation have been extensively characterized at the molecular level in Drosophila. Defects in this process cause sterility in female flies. Early female mouse germ cells are organized as cell clusters as well, but it is uncertain whether these groups are similar to the cysts of invertebrates. We find that mouse germ cells are connected by intercellular bridges in the ovaries of 11.5 to 17.5 days postcoitum embryos; microtubules and organelles have been observed within these bridges. Confocal microscopy shows that cells within mouse clusters divide synchronously and frequently correspond in number to powers of two. Thus, female mouse germ cell clusters exhibit key characteristics of invertebrate germline cysts indicating that the process of germline cyst formation is conserved in the mouse.

scholarly journals Protein Phosphatase 1ß Limits Ring Canal Constriction during Drosophila Germline Cyst Formation

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e70502 ◽  
Author(s):  
Shinya Yamamoto ◽  
Vafa Bayat ◽  
Hugo J. Bellen ◽  
Change Tan
Keyword(s):  
Protein Phosphatase ◽  
Cyst Formation ◽  
Ring Canal ◽  
Germline Cyst

GERMLINE CYST FORMATION IN DROSOPHILA

1997 ◽  
Vol 31 (1) ◽  
pp. 405-428 ◽  
Author(s):  
M de Cuevas ◽  
MA Lilly ◽  
AC Spradling
Keyword(s):  
Cyst Formation ◽  
Germline Cyst

scholarly journals Zebrafish dazl regulates cystogenesis upstream of the meiotic transition and germline stem cell specification and independent of meiotic checkpoints

2019 ◽  
Author(s):  
Sylvain Bertho ◽  
Mara Clapp ◽  
Torsten U. Banisch ◽  
Jan Bandemer ◽  
Erez Raz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Rna Binding ◽  
Process Analysis ◽  
Cyst Formation ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Asymmetric Divisions ◽  
Germline Cyst ◽  
Deleted In Azoospermia

AbstractFertility and gamete reserves are maintained by asymmetric divisions of the germline stem cells to produce new stem cells or daughters that differentiate as gametes. Before entering meiosis, differentiating germ cells (GCs) of sexual animals typically undergo cystogenesis. This evolutionary conserved process involves synchronous and incomplete mitotic divisions of a germ cell daughter (cystoblast) to generate sister cells connected by stable intercellular bridges that facilitate exchange of materials to support a large synchronous population of gamete progenitors. Here we investigate cystogenesis in zebrafish and identified Deleted in azoospermia (Dazl), a conserved vertebrate RNA binding protein as a regulator of this process. Analysis of dazl mutants revealed an essential role for Dazl in regulating incomplete cytokinesis and germline cyst formation before the meiotic transition. Accordingly, dazl mutant GCs form defective ring canals, and ultimately remain as individual cells that fail to differentiate as meiocytes. In addition to promoting cystoblast divisions and meiotic entry, dazl function is required upstream of germline stem cell establishment and fertility.Summary StatementWe show that zebrafish dazl is required for incomplete cytokinesis to generate germline cysts during cystogenesis, acts upstream of germline stem cell establishment, and is required for meiosis, and fertility.

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