Analysis of chromosome 12 aneuploidy in interphase cells from human male germ cell tumors by fluorescence in situ hybridization

1992 ◽  
Vol 5 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Eduardo Rodriguez ◽  
Susan Mathew ◽  
Asit B. Mukherjee ◽  
Victor E. Reuter ◽  
George J. Bosl ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Male Germ Cell ◽  
Chromosome 12 ◽  
Human Male ◽  
Interphase Cells

Detection and analysis of origin of i(12p), a diagnostic marker of human male germ cell tumors, by fluorescence in situ hybridization

1991 ◽  
Vol 3 (4) ◽  
pp. 300-307 ◽  
Author(s):  
Asit B. Mukherjee ◽  
V. V. V. S. Murty ◽  
Eduardo Rodriguez ◽  
Victor E. Reuter ◽  
George J. Bosl ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Diagnostic Marker ◽  
Germ Cell Tumors ◽  
Male Germ Cell ◽  
Human Male

Analysis of chromosome 12 aberrations in human germ cell tumors using fluorescence in situ hybridization

1991 ◽  
Vol 56 (1) ◽  
pp. 95
Author(s):  
R.F. Suijkerbuijk ◽  
R. Sinke ◽  
L.H.J. Looijenga ◽  
B. de Jong ◽  
J.W. Oosterhuis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Chromosome 12

Chromosome 12 Aberrations in Human Germ Cell Tumors: A Fluorescence In Situ Hybridization Inventory

1994 ◽  
pp. 124-137
Author(s):  
R. F. Suijkerbuijk ◽  
R. J. Sinke ◽  
L. H. J. Looijenga ◽  
B. de Jong ◽  
J. W. Oosterhuis ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Chromosome 12

Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two-color fluorescence in situ hybridization (FISH)

1995 ◽  
Vol 14 (4) ◽  
pp. 252-258 ◽  
Author(s):  
Teresa A. Smolarek ◽  
Ruthann I. Blough ◽  
Richard S. Foster ◽  
Thomas M. Ulbright ◽  
Catherine G. Palmer ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Germ Cell ◽  
Fluorescence In Situ Hybridization ◽  
Germ Cell Tumors ◽  
Chromosome 12 ◽  
Testicular Germ Cell Tumors ◽  
Testicular Germ Cell

scholarly journals Detection of trisomy 12 in chronic lymphocytic leukemia by fluorescence in situ hybridization to interphase cells: a simple and sensitive method

Blood ◽  
1992 ◽  
Vol 79 (7) ◽  
pp. 1796-1801 ◽  
Author(s):  
J Anastasi ◽  
MM Le Beau ◽  
JW Vardiman ◽  
AA Fernald ◽  
RA Larson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chronic Lymphocytic Leukemia ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Normal Karyotype ◽  
Lymphocytic Leukemia ◽  
Chromosome 12 ◽  
Interphase Cells ◽  
Trisomy 12

Abstract Trisomy 12 is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL), and a number of studies have suggested that it may be an adverse prognostic indicator. We have evaluated the usefulness of fluorescence in situ hybridization with a chromosome 12- specific probe as a simple means for detecting trisomy 12 in interphase cells. Forty cases of B-cell CLL previously studied with conventional cytogenetic techniques were analyzed with a biotinylated probe to the centromeric region of chromosome 12. Thirty of these retrospective cases could be reevaluated with in situ hybridization. Our analysis showed three hybridization signals (ie, trisomy 12) in interphase cells from seven of seven cases found previously to have trisomy 12. Trisomy 12 was also detected in five additional cases: in one case thought to have a normal karyotype, in two cases that had been inadequate for routine cytogenetic analysis, and in two cases that had been found to have an abnormal karyotype without trisomy 12. In a prospective series of 20 newly accrued CLL cases, all cases were analyzed successfully by in situ hybridization and six (30%) showed trisomy 12. We were able to perform the analysis on routinely prepared and previously Wright- stained peripheral blood smears. We conclude that fluorescence in situ hybridization is a simple means for the detection of trisomy 12 in CLL. The technique is more sensitive than conventional cytogenetic analysis and would be a useful tool in clinical studies.

Cytogenetic and molecular analysis of human male germ cell tumors: Chromosome 12 abnormalities and gene amplification

1990 ◽  
Vol 1 (4) ◽  
pp. 289-300 ◽  
Author(s):  
Felipe Samaniego ◽  
Eduardo Rodriguez ◽  
Jane Houldsworth ◽  
Vundavalli V. V. S. Murty ◽  
Marc Ladanyi ◽  
...  
Keyword(s):  
Germ Cell ◽  
Gene Amplification ◽  
Molecular Analysis ◽  
Germ Cell Tumors ◽  
Male Germ Cell ◽  
Chromosome 12 ◽  
Human Male
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