scholarly journals Genome-wide copy number variation pattern analysis and a classification signature for non-small cell lung cancer

2017 ◽  
Vol 56 (7) ◽  
pp. 559-569 ◽  
Author(s):  
Zhe-Wei Qiu ◽  
Jia-Hao Bi ◽  
Adi F. Gazdar ◽  
Kai Song
Keyword(s):  
Lung Cancer ◽  
Copy Number Variation ◽  
Small Cell Lung Cancer ◽  
Copy Number ◽  
Pattern Analysis ◽  
Small Cell ◽  
Small Cell Lung ◽  
Variation Pattern ◽  
Genome Wide ◽  
Number Variation

Genome-wide combination profiling of DNA copy number and methylation for deciphering biomarkers in non-small cell lung cancer patients

2011 ◽  
Vol 311 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Ji Woong Son ◽  
Kang Jin Jeong ◽  
Woo-Sean Jean ◽  
Soon Young Park ◽  
Sanghoon Jheon ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Patients ◽  
Cell Lung Cancer ◽  
Copy Number ◽  
Small Cell ◽  
Small Cell Lung ◽  
Dna Copy Number ◽  
Lung Cancer Patients ◽  
Genome Wide

scholarly journals Integrative analysis of genome-wide gene copy number changes and gene expression in non-small cell lung cancer

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187246 ◽  
Author(s):  
Verena Jabs ◽  
Karolina Edlund ◽  
Helena König ◽  
Marianna Grinberg ◽  
Katrin Madjar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Copy Number ◽  
Gene Copy Number ◽  
Small Cell ◽  
Gene Copy ◽  
Small Cell Lung ◽  
Genome Wide ◽  
Copy Number Changes

scholarly journals Circulating tumor cell copy-number heterogeneity in ALK-rearranged non-small-cell lung cancer resistant to ALK inhibitors

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Marianne Oulhen ◽  
Patrycja Pawlikowska ◽  
Tala Tayoun ◽  
Marianna Garonzi ◽  
Genny Buson ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Copy Number ◽  
Epithelial To Mesenchymal Transition ◽  
Kinase Inhibitors ◽  
Small Cell ◽  
Small Cell Lung ◽  
Mesenchymal Transition ◽  
Anaplastic Lymphoma

AbstractGatekeeper mutations are identified in only 50% of the cases at resistance to Anaplastic Lymphoma Kinase (ALK)-tyrosine kinase inhibitors (TKIs). Circulating tumor cells (CTCs) are relevant tools to identify additional resistance mechanisms and can be sequenced at the single-cell level. Here, we provide in-depth investigation of copy number alteration (CNA) heterogeneity in phenotypically characterized CTCs at resistance to ALK-TKIs in ALK-positive non-small cell lung cancer. Single CTC isolation and phenotyping were performed by DEPArray or fluorescence-activated cell sorting following enrichment and immunofluorescence staining (ALK/cytokeratins/CD45/Hoechst). CNA heterogeneity was evaluated in six ALK-rearranged patients harboring ≥ 10 CTCs/20 mL blood at resistance to 1st and 3rd ALK-TKIs and one presented gatekeeper mutations. Out of 82 CTCs isolated by FACS, 30 (37%) were ALK+/cytokeratins-, 46 (56%) ALK-/cytokeratins+ and 4 (5%) ALK+/cytokeratins+. Sequencing of 43 CTCs showed highly altered CNA profiles and high levels of chromosomal instability (CIN). Half of CTCs displayed a ploidy >2n and 32% experienced whole-genome doubling. Hierarchical clustering showed significant intra-patient and wide inter-patient CTC diversity. Classification of 121 oncogenic drivers revealed the predominant activation of cell cycle and DNA repair pathways and of RTK/RAS and PI3K to a lower frequency. CTCs showed wide CNA heterogeneity and elevated CIN at resistance to ALK-TKIs. The emergence of epithelial ALK-negative CTCs may drive resistance through activation of bypass signaling pathways, while ALK-rearranged CTCs showed epithelial-to-mesenchymal transition characteristics potentially contributing to ALK-TKI resistance. Comprehensive analysis of CTCs could be of great help to clinicians for precision medicine and resistance to ALK-targeted therapies.

scholarly journals Classification of Non-Small Cell Lung Cancer Based on Copy Number Alterations

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e88300 ◽  
Author(s):  
Bi-Qing Li ◽  
Jin You ◽  
Tao Huang ◽  
Yu-Dong Cai
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Copy Number ◽  
Small Cell ◽  
Copy Number Alterations ◽  
Small Cell Lung

scholarly journals Mutation analysis and copy number alterations of KIF23 in non-small-cell lung cancer exhibiting KIF23 over-expression

2017 ◽  
Vol Volume 10 ◽  
pp. 4969-4979 ◽  
Author(s):  
Ann-Louise Vikberg ◽  
Tõnu Vooder ◽  
Kaie Lokk ◽  
Tarmo Annilo ◽  
Irina Golovleva
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Mutation Analysis ◽  
Cell Lung Cancer ◽  
Copy Number ◽  
Small Cell ◽  
Copy Number Alterations ◽  
Small Cell Lung ◽  
Over Expression
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