Homozygous deletions at 3p22, 5p14, 6q15, and 9p21 result in aberrant expression of tumor suppressor genes in gastric cancer

2014 ◽  
Vol 54 (3) ◽  
pp. 142-155 ◽  
Author(s):  
Bona Lee ◽  
Kwiyeom Yoon ◽  
Sunghoon Lee ◽  
Jin Muk Kang ◽  
Junil Kim ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Aberrant Expression ◽  
Suppressor Genes ◽  
Homozygous Deletions

Association Between Cyclin D1 Polymorphism with CpG Island Promoter Methylation Status of Tumor Suppressor Genes in Gastric Cancer

2010 ◽  
Vol 55 (12) ◽  
pp. 3449-3457 ◽  
Author(s):  
Tomomitsu Tahara ◽  
Tomoyuki Shibata ◽  
Masakatsu Nakamura ◽  
Hiromi Yamashita ◽  
Daisuke Yoshioka ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Cyclin D1 ◽  
Promoter Methylation ◽  
Tumor Suppressor Genes ◽  
Cpg Island ◽  
Methylation Status ◽  
Suppressor Genes ◽  
Promoter Methylation Status

scholarly journals Promoter Methylation of RASSF1A Associates to Adult Secondary Glioblastomas and Pediatric Glioblastomas

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jorge Muñoz ◽  
María del Mar Inda ◽  
Paula Lázcoz ◽  
Idoya Zazpe ◽  
Xing Fan ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Cell Lines ◽  
Tumor Suppressor Genes ◽  
Promoter Hypermethylation ◽  
Suppressor Genes ◽  
Primary Tumors ◽  
Specific Pcr ◽  
Inactivation Mechanism ◽  
Altered Gene ◽  
Homozygous Deletions

While allelic losses and mutations of tumor suppressor genes implicated in the etiology of astrocytoma have been widely assessed, the role of epigenetics is still a matter of study. We analyzed the frequency of promoter hypermethylation by methylation-specific PCR (MSP) in five tumor suppressor genes (PTEN, MGMT, RASSF1A, p14ARF, and p16INK4A), in astrocytoma samples and cell lines. RASSF1A was the most frequently hypermethylated gene in all grades of astrocytoma samples, in cell lines, and in adult secondary GBM. It was followed by MGMT. PTEN showed a slight methylation signal in only one GBM and one pilocytic astrocytoma, and in two cell lines; while p14ARF and p16INK4A did not show any evidence of methylation in primary tumors or cell lines. In pediatric GBM, RASSF1A was again the most frequently altered gene, followed by MGMT; PTEN, p14 and p16 showed no alterations. Lack or reduced expression of RASSF1A in cell lines was correlated with the presence of methylation. RASSF1A promoter hypermethylation might be used as a diagnostic marker for secondary GBM and pediatric GBM. Promoter hypermethylation might not be an important inactivation mechanism in other genes like PTEN, p14ARF and p16INK4A, in which other alterations (mutations, homozygous deletions) are prevalent.

Aberrant expression of tumor suppressor genes and their association with chimeric oncogenes in pediatric acute lymphoblastic leukemia

2005 ◽  
Vol 29 (11) ◽  
pp. 1271-1276 ◽  
Author(s):  
Anatoly M. Kustanovich ◽  
Tatjana V. Savitskaja ◽  
Oleg I. Bydanov ◽  
Michael V. Belevtsev ◽  
Michael P. Potapnev
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Lymphoblastic Leukemia ◽  
Aberrant Expression ◽  
Suppressor Genes ◽  
Pediatric Acute Lymphoblastic Leukemia

scholarly journals GLAD-PCR assay of R(5mC)GY sites in the regulatory region of tumor-suppressor genes associated with gastric cancer

Acta Naturae ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 124-133
Author(s):  
Boris S. Malyshev ◽  
Nina A. Netesova ◽  
Natalia A. Smetannikova ◽  
Murat A. Abdurashitov ◽  
Alexander G. Akishev ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Dna Methyltransferase ◽  
Regulatory Region ◽  
Pcr Assay ◽  
Suppressor Genes ◽  
Regulatory Regions ◽  
Tissue Samples ◽  
Diagnostic Potential

At early stages of carcinogenesis, the regulatory regions of some tumor suppressor genes become aberrantly methylated at RCGY sites, which are substrates of DNA methyltransferase Dnmt3. Identification of aberrantly methylated sites in tumor DNA is considered to be the first step in the development of epigenetic PCR test systems for early diagnosis of cancer. Recently, we have developed a GLAD-PCR assay, a method for detecting the R(5mC)GY site in the genome position of interest even at significant excess of DNA molecules with a non-methylated RCGY site in this location. The aim of the present work is to use the GLAD-PCR assay to detect the aberrantly methylated R(5mC)GY sites in the regulatory regions of tumor suppressor genes (brinp1, bves, cacna2d3, cdh11, cpeb1, epha7, fgf2, galr1, gata4, hopx, hs3st2, irx1, lrrc3b, pcdh10, rprm, runx3, sfrp2, sox17, tcf21, tfpi2, wnt5a, zfp82, and znf331) in DNA samples obtained from gastric cancer (GC) tissues. The study of the DNA samples derived from 29 tumor and 25 normal gastric tissue samples demonstrated a high diagnostic potential of the selected RCGY sites in the regulatory regions of the irx1, cacna2d3, and epha7 genes; the total indices of sensitivity and specificity for GC detection being 96.6% and 100%, respectively.

Increased Number of CpG Island Hypermethylation in Tumor Suppressor Genes of Non-Neoplastic Gastric Mucosa Correlates with Higher Risk of Gastric Cancer

Digestion ◽  
2010 ◽  
Vol 82 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Tomomitsu Tahara ◽  
Tomoyuki Shibata ◽  
Masakatsu Nakamura ◽  
Hiromi Yamashita ◽  
Daisuke Yoshioka ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Mucosa ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Cpg Island ◽  
Suppressor Genes

scholarly journals Exclusive Association of p53 Mutation with Super-High Methylation of Tumor Suppressor Genes in the p53 Pathway in a Unique Gastric Cancer Phenotype

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139902 ◽  
Author(s):  
Mina Waraya ◽  
Keishi Yamashita ◽  
Akira Ema ◽  
Natsuya Katada ◽  
Shiro Kikuchi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
P53 Mutation ◽  
Suppressor Genes ◽  
P53 Pathway ◽  
Cancer Phenotype

Epigenetic-mediated tumor suppressor genes as diagnostic or prognostic biomarkers in gastric cancer

2013 ◽  
Vol 13 (5) ◽  
pp. 445-455 ◽  
Author(s):  
Koji Otani ◽  
Xiaoxing Li ◽  
Tetsuo Arakawa ◽  
Francis KL Chan ◽  
Jun Yu
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Prognostic Biomarkers ◽  
Suppressor Genes
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