Telomere-mediated mitotic disturbances in immortalized ovarian epithelial cells reproduce chromosomal losses and breakpoints from ovarian carcinoma

2004 ◽  
Vol 42 (1) ◽  
pp. 22-33 ◽  
Author(s):  
David Gisselsson ◽  
Mei Lv ◽  
Sai-Wha Tsao ◽  
Cornelia Man ◽  
Charlotte Jin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ovarian Carcinoma ◽  
Ovarian Epithelial Cells ◽  
Mitotic Disturbances

scholarly journals Application of Magnetic Resonance Image Intelligent Data Acquisition in Ovarian Cancer (Preprint)

2020 ◽  
Author(s):  
Tingting Liang ◽  
Yanlei Dong ◽  
Xinrui Zhao ◽  
Lu Wang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Magnetic Resonance ◽  
Epithelial Cells ◽  
Data Acquisition ◽  
Cell Lines ◽  
P53 Protein ◽  
Control Group ◽  
Ovarian Epithelial Cells ◽  
Intelligent Data Acquisition ◽  
Experimental Group

UNSTRUCTURED As a diagnostic method with no radiation, high resolution of soft tissue, and different imaging methods, Magnetic Resonance Imaging (MRI) intelligent data acquisition is more and more widely used in the examination of abdominal, pelvic, and other organ lesions. In order to study the diagnostic effect of multi-mode magnetic resonance intelligent data acquisition on ovarian cancer and the ovarian cancer model modified based on p53-/-+Myc+ASAP1 gene, NSG mice were selected as experimental subjects in this study. 293FT cell lines packaging p53, Myc, and ASAP1(ArfGAP with SH3 domain, Ankyrin repeat and PH domain 1) recombinant lentivirus were inoculated into mouse ovarian epithelial cells to construct mouse ovarian epithelial cell tumor cell lines and their performance was analyzed. According to the different injection cell lines, they were divided into the experimental group and the control group. Tumor samples were collected and the mice were analyzed using immunofluorescence staining and MRI. The results showed that, in the detection of protein expression in genetically modified cell lines, for p53-/-+Myc+ASAP1 fully modified cell lines, the high expression of ASAP1 and Myc functional proteins was detected after the lentivirus containing p53-/-, ASAP1, and Myc were introduced into mouse ovarian epithelial cells, while the expression of p53 protein decreased significantly; after inoculation into mice, it was found that the expression of ASAP1 protein and Myc protein in the experimental group was significantly higher than that in the control group, while the expression of p53 protein in the experimental group was significantly lower than that in the control group, with significant statistical difference; further MRI diagnosis of two groups of mice showed that the ADC (Apparent dispersion coefficient) value of the experimental group was significantly higher than the control group, there were statistically significant differences. Therefore, it was found that p53 gene expression was down-regulated and Myc and ASAPl genes were overexpressed in the tumor tissues and tumor cells formed, and tumor formation differences between the two groups of mice could be obviously found after MRI intelligent data acquisition, which provided experimental basis for early diagnosis of breast cancer in the later clinical stage.

scholarly journals Knockdown of CYP24A1 Aggravates 1α,25(OH)2D3-Inhibited Migration and Invasion of Mouse Ovarian Epithelial Cells by Suppressing EMT

2020 ◽  
Vol 10 ◽  
Author(s):  
Ping Wang ◽  
Jiming Xu ◽  
Weijing You ◽  
Yongfeng Hou ◽  
Shuiliang Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Migration And Invasion ◽  
Ovarian Epithelial Cells

scholarly journals Migration dynamics of ovarian epithelial cells on micro-fabricated image-based models of normal and malignant stroma

2019 ◽  
Vol 100 ◽  
pp. 92-104 ◽  
Author(s):  
Samuel Alkmin ◽  
Rebecca Brodziski ◽  
Haleigh Simon ◽  
Daniel Hinton ◽  
Randall H. Goldsmith ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Migration Dynamics ◽  
Ovarian Epithelial Cells

scholarly journals Biologic effects of heregulin/neu differentiation factor on normal and malignant human breast and ovarian epithelial cells

Oncogene ◽  
1999 ◽  
Vol 18 (44) ◽  
pp. 6050-6062 ◽  
Author(s):  
Zuleima Aguilar ◽  
Robert W Akita ◽  
Richard S Finn ◽  
B Lillian Ramos ◽  
Mark D Pegram ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Breast ◽  
Differentiation Factor ◽  
Biologic Effects ◽  
Ovarian Epithelial Cells

Molecular mechanisms of isocyanate induced oncogenic transformation in ovarian epithelial cells

2010 ◽  
Vol 30 (3) ◽  
pp. 377-386 ◽  
Author(s):  
Gorantla Venkata Raghuram ◽  
Neelam Pathak ◽  
Deepika Jain ◽  
Hariom Panwar ◽  
Hemant Pandey ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Molecular Mechanisms ◽  
Oncogenic Transformation ◽  
Ovarian Epithelial Cells
Sign in / Sign up

Export Citation Format

Share Document