scholarly journals Effect of pinolenic acid on oxidative stress injury in HepG2 cells induced by H 2 O 2

2021 ◽  
Author(s):  
Yang Zhao ◽  
Sainan Liu ◽  
Zhili Sheng ◽  
Xue Li ◽  
Yanan Chang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Pinolenic Acid ◽  
Stress Injury ◽  
Oxidative Stress Injury

scholarly journals Active Fragment ofVeronica ciliataFisch. Attenuates t-BHP-Induced Oxidative Stress Injury in HepG2 Cells through Antioxidant and Antiapoptosis Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Yiran Sun ◽  
Qiuxia Lu ◽  
Libo He ◽  
Yueyue Shu ◽  
Shiyan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Cell Injury ◽  
Hepatoprotective Activity ◽  
The Body ◽  
Protein Carbonyls ◽  
Stress Injury ◽  
Oxidative Stress Injury ◽  
Active Fragment

Excessive amounts of reactive oxygen species (ROS) in the body are a key factor in the development of hepatopathies such as hepatitis. The aim of this study was to assess the antioxidation effect in vitro and hepatoprotective activity of the active fragment ofVeronica ciliataFisch. (VCAF). Antioxidant assays (DPPH, superoxide, and hydroxyl radicals scavenging) were conducted, and hepatoprotective effects through the application oftert-butyl hydroperoxide- (t-BHP-) induced oxidative stress injury in HepG2 cells were evaluated. VCAF had high phenolic and flavonoid contents and strong antioxidant activity. From the perspective of hepatoprotection, VCAF exhibited a significant protective effect on t-BHP-induced HepG2 cell injury, as indicated by reductions in cytotoxicity and the levels of ROS, 8-hydroxydeoxyguanosine (8-OHdG), and protein carbonyls. Further study demonstrated that VCAF attenuated the apoptosis of t-BHP-treated HepG2 cells by suppressing the activation of caspase-3 and caspase-8. Moreover, it significantly decreased the levels of ALT and AST, increased the activities of acetyl cholinesterase (AChE), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), and increased total antioxidative capability (T-AOC). Collectively, we concluded that VCAF may be a considerable candidate for protecting against liver injury owing to its excellent antioxidant and antiapoptosis properties.

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