scholarly journals Anti‐arthritic effect of chicken embryo tissue hydrolyzate against adjuvant arthritis in rats (X‐ray microtomographic and histopathological analysis)

2021 ◽  
Author(s):  
Igor Rzhepakovsky ◽  
Shahida Anusha Siddiqui ◽  
Svetlana Avanesyan ◽  
Mehmet Benlidayi ◽  
Kunaal Dhingra ◽  
...  
Keyword(s):  
Chicken Embryo ◽  
Adjuvant Arthritis ◽  
Histopathological Analysis ◽  
X Ray ◽  
Embryo Tissue

scholarly journals Anti-Arthritic Effect of Chicken Embryo Tissue Hydrolyzate Against Adjuvant Arthritis in Rats (X-Ray Microtomographic and Histopathological Analysis)

2020 ◽  
Author(s):  
Igor Rzhepakovsky ◽  
Svetlana Avanesyan ◽  
Mehmet Benlidayi ◽  
Kunaal Dhingra ◽  
Alexander Dolgalev ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oral Administration ◽  
Chicken Embryo ◽  
Morphological Changes ◽  
Diclofenac Sodium ◽  
Cartilage Degeneration ◽  
Pathological Process ◽  
Chronic Arthritis ◽  
Histopathological Analysis ◽  
X Ray

Finding new, safe strategies to prevent and control rheumatoid arthritis is an urgent task. Of particular interest in this regard are bioactive peptides and peptide-rich protein hydrolyzates, which represent a new trend in the development of functional foods and nutraceuticals. The resulting tissue hydrolyzate of the chicken embryo (CETH) has been evaluated for acute toxicity and tested against chronic arthritis induced by Freund's full adjuvant in rats. The anti-arthritic effect of CETH was studied on the 28th day of the experiment after two weeks of oral administration of CETH at doses of 60 and 120 mg/kg body weight. Arthritis was evaluated on the last day of the experiment on the injected animal paw using X-ray computerized microtomography and histopathology analysis methods. The CETH effect was compared with the non-steroidal anti-inflammatory drug diclofenac sodium (5 mg/kg). Oral administration of CETH was accompanied by effective dose-dependent correction of morphological changes caused by the adjuvant injection. CETH had relatively high recovery effects in terms of parameters for reducing inflammatory edema, inhibition of osteolysis, prevention of osteophitosis, reduction of the inflammatory reaction of periarticular tissues, and cartilage degeneration. This study presents a potential theoretical strategy for the safe correction of this pathological process and, for the first time, shows that CETH may be a powerful potential nutraceutical agent or bioactive component of functional products in the treatment of rheumatoid arthritis.

scholarly journals Cryptococcosis, silicosis, and tuberculous pseudotumor in the same pulmonary lobe

2013 ◽  
Vol 39 (5) ◽  
pp. 620-626 ◽  
Author(s):  
Geruza Alves da Silva ◽  
Daniel Ferracioli Brandao ◽  
Elcio Oliveira Vianna ◽  
Joao Batista Carlos de Sa Filho ◽  
Jose Baddini-Martinez
Keyword(s):  
Surgical Approach ◽  
Opportunistic Infections ◽  
Histopathological Analysis ◽  
Noninvasive Methods ◽  
X Ray ◽  
History Of ◽  
Chest X Ray ◽  
Atypical Presentations ◽  
The Right ◽  
Surrounding Parenchyma

Tuberculosis and cryptococcosis are infectious diseases that can result in the formation of single or multiple nodules in immunocompetent patients. Exposure to silica is known to raise the risk of infection with Mycobacterium tuberculosis. We report the case of an elderly man with no history of opportunistic infections and no clinical evidence of immunodeficiency but with a six-month history of dry cough and nocturnal wheezing. A chest X-ray revealed a mass measuring 5.0 × 3.5 cm in the right upper lobe. The diagnostic approach of the mass revealed tuberculosis. The histopathological analysis of the surrounding parenchyma reveled silicosis and cryptococcosis. Cryptococcosis was also found in masses identified in the mediastinal lymph nodes. The surgical approach was indicated because of the degree of pleuropulmonary involvement, the inconclusive results obtained with the invasive and noninvasive methods applied, and the possibility of malignancy. This case illustrates the difficulty inherent to the assessment of infectious or inflammatory pulmonary pseudotumors, the differential diagnosis of which occasionally requires a radical surgical approach. Despite the presence of respiratory symptoms for six months, the first chest X-ray was performed only at the end of that period. We discuss the possible pathogenic mechanisms that might have led to the combination of three types of granulomatous lesions in the same lobe, and we emphasize the need for greater awareness of atypical presentations of pulmonary tuberculosis.

scholarly journals Hypertrophic Nonunion Humerus Mimicking an Enchondroma

2014 ◽  
Vol 2014 ◽  
pp. 1-4
Author(s):  
N. K. Magu ◽  
Amanpreet Singh ◽  
Reetadyuti Mukhopadhyay ◽  
Jitendra Wadhwani ◽  
Paritosh Gogna ◽  
...  
Keyword(s):  
Case Report ◽  
Conservative Management ◽  
Young Male ◽  
Humeral Shaft ◽  
Histopathological Analysis ◽  
Diagnostic Dilemma ◽  
X Ray ◽  
Benign Tumours

Introduction. Although fractures of humeral shaft show excellent results with conservative management, nonunion does occur.Case Report. We bring forth the case of a young male with a 1.5-year-old hypertrophic nonunion of the humerus mimicking an enchondroma. The initial X-ray images of the patient appeared to be an enchondroma, which only on further evaluation and histopathological analysis was diagnosed conclusively to be a hypertrophic nonunion.Discussion. Enchondromas are often incidentally diagnosed benign tumours. It is however not common to misdiagnose a hypertrophic nonunion to be an enchondroma. We present this case to highlight the unique diagnostic dilemma the treating team had to face.

Slc7a9knockout mouse is a good cystinuria model for antilithiasic pharmacological studies

2007 ◽  
Vol 293 (3) ◽  
pp. F732-F740 ◽  
Author(s):  
Mariona Font-Llitjós ◽  
Lídia Feliubadaló ◽  
Meritxell Espino ◽  
Ramon Clèries ◽  
Sandra Mañas ◽  
...  
Keyword(s):  
Amino Acids ◽  
Mouse Model ◽  
Mutant Mice ◽  
New Drugs ◽  
Histopathological Analysis ◽  
Knockout Mouse Model ◽  
X Ray ◽  
Pharmacological Studies ◽  
Before And After ◽  
Dibasic Amino Acids

Cystinuria is a hereditary disorder caused by a defect in the apical membrane transport system for cystine and dibasic amino acids in renal proximal tubules and intestine, resulting in recurrent urolithiasis. Mutations in SLC3A1 and SLC7A9 genes, that codify for rBAT/b0,+AT transporter subunits, cause type A and B cystinuria, respectively. In humans, cystinuria treatment is based on the prevention of calculi formation and its dissolution or breakage. Persistent calculi are treated with thiols [i.e., d-penicillamine (DP) and mercaptopropionylglycine (MPG)] for cystine solubilization. We have developed a new protocol with DP to validate our Slc7a9 knockout mouse model for the study of the therapeutic effect of drugs in the treatment of cystine lithiasis. We performed a 5-wk treatment of individually caged lithiasic mutant mice with a previously tested DP dose. To appraise the evolution of lithiasis throughout the treatment a noninvasive indirect method of calculi quantification was developed: calculi mass was quantified by densitometry of X-ray images from cystinuric mice before and after treatment. Urine was collected in metabolic cage experiments to quantify amino acids in DP-treated and nontreated, nonlithiasic mutant mice. We found significant differences between DP-treated and nontreated knockout mice in calculi size and in urinary cystine excretion. Histopathological analysis showed that globally nontreated mutant mice had more severe and diffuse urinary system damage than DP-treated mice. Our results validate the use of this mouse model for testing the efficacy of potential new drugs against cystinuria.

scholarly journals Hydrogel with silver nanoparticles synthesized by Mimosa tenuiflora for second-degree burns treatment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aaron Martínez-Higuera ◽  
César Rodríguez-Beas ◽  
Jesús Mauro Adolfo Villalobos-Noriega ◽  
Abraham Arizmendi-Grijalva ◽  
Carlos Ochoa-Sánchez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Burn Injury ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
X Ray ◽  
Burn Wound Healing ◽  
Mimosa Tenuiflora ◽  
Second Degree

AbstractIn this work we use Mimosa tenuiflora (MtE) extracts as reducing agents to synthesize silver nanoparticles (AgMt NPs) which were characterized by DPPH and Total Polyphenols Assays, UV–visible, X-ray diffractometer (XRD), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and Thermogravimetric analysis (TGA). AgMt NPs possess average sizes of 21 nm and fcc crystalline structure, it was also confirmed that the MtE is present in the AgMt NPs even after the cleaning protocol applied. Subsequently, carbopol hydrogels were made and the MtE and the synthesized AgMt NPs were dispersed in different gels (MtE-G and AgMt NPs-G, respectively) at 100 µg/g concentration. The gels were characterized by UV–Vis, IR, and rheology. Antimicrobial tests were performed using Staphylococcus aureus and Escherichia coli. Burn wound healing was evaluated in a second-degree burn injury on a Wistar rats model for 14 days and additional skin biopsies were examined with histopathological analysis. Gel with commercial silver nanoparticles (Ag NPs) was prepared and employed as a control on the biological assays. Hydrogel system containing silver nanoparticles synthesized with Mimosa tenuiflora (AgMt NPs-G) is a promising therapeutic strategy for burn wound healing, this due to bactericidal and anti-inflammatory effects, which promotes a more effective recovery (in percentage terms) by damaged area.

scholarly journals Six-month prospective trial in early and long-standing rheumatoid arthritis: evaluating disease activity in the wrist through sequential synovial histopathological analysis, RAMRIS magnetic resonance score and EULAR-OMERACT ultrasound score

RMD Open ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. e000951 ◽  
Author(s):  
Søren Andreas Just ◽  
Christian Nielsen ◽  
Jens Christian Werlinrud ◽  
Pia Veldt Larsen ◽  
Camilla Schufri Klinkby ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Magnetic Resonance ◽  
Prospective Study ◽  
Disease Activity ◽  
Synovial Biopsy ◽  
Histopathological Analysis ◽  
X Ray ◽  
Larsen Score ◽  
Synovitis Score ◽  
Radiocarpal Joint

IntroductionStandardised scoring systems for rheumatoid arthritis (RA) joint disease activity include Larsen score for radiographs, rheumatoid arthritis magnetic resonance imaging score (RAMRIS) for MRI and using the European League Against Rheumatisms-Outcome Measures in Rheumatology (EULAR-OMERACT) score for ultrasound (US) images. The aim of this prospective study was to investigate the relationship between histological synovitis and radiological synovitis, assessed by conventional X-ray, US and MRI of the wrist radiocarpal joint.Methods20 patients with treatment naive early RA (ERA) and 20 with long-standing RA (LRA) were enrolled in a 6-month prospective study. Patients with RA underwent US-guided synovial biopsy, X-ray and US of the wrist at enrolment and 6 months. MRI at baseline and also at 6 months for the ERA group, and scored with the RAMRIS system. X-ray was scored by Larsen score and US by the EULAR-OMERACT system. Synovial biopsy inflammation was determined by the Krenn score.ResultsIn the ERA group at baseline, Krenn score was correlated strongly with both US combined score (r = 0.77 p < 0.001) and MRI synovitis score (r = 0.85 p < 0.001), while uncorrelated at 6 months. In the LRA group at baseline, these scores correlated strongly (r = 0.83, p < 0.001) to moderately (r = 0.61, p = 0.002), and persisted at 6 months for US score (r = 0.81 p < 0.001). For all patients with RA, change in Krenn score between baseline and 6 months was correlated with both change in US combined score (r = 0.65, p < 0.001) and change in MRI synovitis score (r = 0.50, p = 0.03).ConclusionThe MRI RAMRIS synovitis score and EULAR-OMERACT US scoring system are sensitive measures of histological synovitis in LRA and ERA. After 6 months, this correlation persists in the established RA group, but not in the ERA group. Overall, decreases in MRI/US synovitis are associated with reductions in histological synovitis. The study validates the use of MRI RAMRIS and EULAR-OMERACT US scores as surrogate markers of histological synovitis in established RA and early untreated RA.

scholarly journals BI-RADS classification and gray level histogram of malignant mammary tumors in bitches

2018 ◽  
Vol 38 (10) ◽  
pp. 1942-1948
Author(s):  
Débora M.N.M. Oliveira ◽  
Fabiano S. Costa ◽  
Aurea Wischral
Keyword(s):  
Mammary Tumors ◽  
Abdominal Ultrasound ◽  
Histopathological Analysis ◽  
Neoplastic Tissue ◽  
X Ray ◽  
Positive Correlation ◽  
Image Characteristics ◽  
Grade 3 ◽  
Gray Level Histogram ◽  
Base Width

ABSTRACT: Mammary tumor is the most frequent among the tumors that affect canine females, with relevant importance in veterinary medicine. The objective of this study was to determine the image characteristics of mammary tumors in female dogs, and compare different ultrasonographic techniques for neoplastic evaluation. During the experiment, 30 bitches with presence of nodular lesion in the mammary gland were used. Initially females were submitted to clinical and laboratory evaluations, and subsequent to the ultrasound examination of the tumor mass, as well as abdominal ultrasound and thoracic x-ray for the metastasis investigation. Quantitative analysis by histogram of the gray levels and categorization of the tumor masses by the BI-RADS system were performed. Later, the bitches were submitted to surgical resection of the tumors, where samples of the neoplastic tissue were collected for histopathological analysis. Carcinoma in mixed tumor showed a higher rate (33.3%), and the malignancy degree of epidermal tumors were classified in grade 1 (n=9), grade 2 (n=12) and grade 3 (n=3). Malignancy degree showed positive correlation with BI-RADS (r=0.55; P<0.05) and with the parameter echotexture - histogram base width (r=0.42, P<0.05). BI-RADS graduation also showed a positive correlation with the echotexture parameters (standard deviation of average echogenicity r=0.66, P<0.05 and base width r=0.55, P<0.05). It was concluded that the BI-RADS method in combination with the echotexture of tumors, can be used to evaluate mammary tumors in dogs and establish the planning of treatment.

Towards histopathological analysis based on X-ray fluorescence elemental imaging supported by multivariate analysis - Case study of ovarian cancers

2019 ◽  
Vol 155 ◽  
pp. 4-11
Author(s):  
Paweł M. Wróbel ◽  
Łukasz Chmura ◽  
Maria M. Grzelak ◽  
Zdzisław Stęgowski ◽  
Marek Lankosz ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Histopathological Analysis ◽  
X Ray ◽  
Elemental Imaging ◽  
Ovarian Cancers

An X-ray analysis of adjuvant arthritis in the rat. The effect of prednisolone and indomethacin

1977 ◽  
Vol 7 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Alan Blackham ◽  
John W. Burns ◽  
John B. Farmer ◽  
Henryk Radziwonik ◽  
John Westwick
Keyword(s):  
Adjuvant Arthritis ◽  
X Ray
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