scholarly journals Investigation of antimicrobial susceptibility and virulence factor genes in Trueperella pyogenes isolated from clinical mastitis cases of dairy cows

2021 ◽  
Author(s):  
Iradj Ashrafi Tamai ◽  
Abdolmajid Mohammadzadeh ◽  
Taghi Zahraei Salehi ◽  
Pezhman Mahmoodi ◽  
Babak Pakbin
Keyword(s):  
Dairy Cows ◽  
Virulence Factor ◽  
Antimicrobial Susceptibility ◽  
Clinical Mastitis ◽  
Trueperella Pyogenes ◽  
Virulence Factor Genes

scholarly journals Microbial Aetiology, Antibiotic Susceptibility and Pathogen-Specific Risk Factors for Udder Pathogens from Clinical Mastitis in Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2113
Author(s):  
Anna Duse ◽  
Karin Persson-Waller ◽  
Karl Pedersen
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Dairy Cows ◽  
Antibiotic Susceptibility ◽  
Clinical Mastitis ◽  
Gram Positive ◽  
E Coli ◽  
Trueperella Pyogenes ◽  
Klebsiella Spp

Mastitis is one of the most important infectious diseases and one of the diseases that causes the greatest use of antibiotics in dairy cows. Therefore, updated information on the bacteria that cause mastitis and their antibiotic susceptibility properties is important. Here, for the first time in over 10 years, we updated the bacterial findings in clinical mastitis in Swedish dairy cows together with their antibiotic resistance patterns and risk factors for each bacterial species. During the period 2013–2018, samples from clinical mastitis were collected, together with information on the cows and herds of origin. The samples were cultured, and a total of 664 recovered bacterial isolates were subjected to susceptibility testing. Staphylococcus aureus (S. aureus) was the most common pathogen and accounted for 27.8% of diagnoses, followed by Streptococcus dysgalactiae (S. dysgalactiae) (15.8%), Escherichia coli (E. coli) (15.1%), Streptococcus uberis (S. uberis) (11.4%), Trueperella pyogenes (T. pyogenes) (7.7%), non-aureus staphylococci (NAS) (2.8%), Klebsiella spp. (2.7%), Enterococcus spp. (1.3%), and Streptococcus agalactiae (S. agalactiae) (1.2%). Various other bacteria accounted for 2.6%. Staphylococci were, in general, susceptible to most antibiotics, but 2.6% of S. aureus and 30.4% of NAS were resistant to penicillin. No methicillin-resistant staphylococci were found. All S. agalactiae were susceptible to penicillin. Bimodal and trimodal MIC distributions for penicillin in S. dysgalactiae and S. uberis, respectively, indicate acquired reduced susceptibility in some isolates. The mostly unimodal MIC distributions of T. pyogenes indicate that acquired resistance does usually not occur in this species. Among E. coli, 14.7% were resistant to at least one antibiotic, most often ampicillin (8.7%), streptomycin (7.8%), or sulphamethoxazole (6.9%). Klebsiella spp. had low resistance to tetracycline (9.1%) but is considered intrinsically resistant to ampicillin. Pathogen-specific risk factors were investigated using multivariable models. Staphylococcus aureus, S. dysgalactiae, and T. pyogenes were more common, while E. coli was less common in quarters with more than one pathogen. S. aureus and T. pyogenes were mostly seen in early lactation, while E. coli was more common in peak to mid lactation and S. dysgalactiae in early to peak lactation. Trueperella pyogenes and Klebsiella spp. were associated with a previous case of clinical mastitis in the current lactation. Staphylococcus aureus was associated with tie stalls and T. pyogenes with loose housing. All pathogens except E. coli and S. dysgalactiae had a seasonal distribution. In conclusion, the aetiological agents for clinical bovine mastitis have remained relatively stable over the last 10–15 years, S. aureus, S. dysgalactiae, E. coli and S. uberis being the most important. Resistance to penicillin among Gram-positive agents was low, and in general, antibiotic resistance to other compounds was low among both Gram-positive and Gram-negative agents.

scholarly journals Antimicrobial Resistance and Virulence Genes Distribution in Trueperella pyogenes Isolated from Dairy Cows with Clinical Mastitis in Liaoning of China

2021 ◽  
Vol 41 (03) ◽  
pp. 329-334
Author(s):  
Yuru Guo
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Gene Cassette ◽  
Clinical Mastitis ◽  
Liaoning Province ◽  
Chain Reactions ◽  
Trueperella Pyogenes ◽  
Broth Microdilution Method

Trueperella pyogenes is considered as a causative agent of many infections, such as mastitis, endometritis, pneumonia, liver abscessation. T. pyogenes can express several virulence genes such as plo, fimA, cbpA, nanH and nanP contributing to its pathogenicity. The aim of this study was to provide an investigation about antimicrobial resistance, as well as virulence genes distribution and gene cassettes among T. pyogenes isolates from dairy cows with clinical mastitis. The susceptibility to different antimicrobial agents was determined by the Broth Microdilution Method, and virulence genes and gene cassette was detected by polymerase chain reactions (PCRs). There are 10.49% (17/162) of milk samples from dairy cows with mastitis were positive for T. pyogenes. High levels of resistance were found to clindamycin (23.53%), oxytetracycline (23.53%), ciprofloxacin (47.06%), sulfamethoxazole/trimethoprim (100%). Moreover, all isolates carried class I integrons, and gene cassette arrays were aadA9 (2/17) or aadA5-dfrA17 (3/17). Finally, all isolates harbored plo nanH and fimA genes, but other genes encoding virulence genes including fimC, fimE, nanP and cbpA are ranged from 47.06% to 88.23%. Our study showed T. pyogenes isolates from dairy cows with clinical mastitis were susceptible to β-lactams. In addition, all seven virulence genes occurred in isolates, and plo, nanH, and fimA gene showed a significantly higher frequency in T. pyogenes of the Liaoning Province, China.

Monitoring of antimicrobial susceptibility of udder pathogens recovered from cases of clinical mastitis in dairy cows across Europe: VetPath results

2018 ◽  
Vol 213 ◽  
pp. 73-81 ◽  
Author(s):  
Anno de Jong ◽  
Farid El Garch ◽  
Shabbir Simjee ◽  
Hilde Moyaert ◽  
Markus Rose ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Antimicrobial Susceptibility ◽  
Clinical Mastitis

Antimicrobial resistance and presence of virulence factor genes in Trueperella pyogenes isolated from pig lungs with pneumonia

2019 ◽  
Vol 51 (7) ◽  
pp. 2099-2103 ◽  
Author(s):  
Wen-Long Dong ◽  
Lei Liu ◽  
Kokou Ayefounin Odah ◽  
Luke Atiewin Atiah ◽  
Yun-Hang Gao ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Virulence Factor ◽  
Trueperella Pyogenes ◽  
Virulence Factor Genes

Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows

2009 ◽  
Vol 136 (1-2) ◽  
pp. 142-149 ◽  
Author(s):  
Björn Bengtsson ◽  
Helle Ericsson Unnerstad ◽  
Torkel Ekman ◽  
Karin Artursson ◽  
Maria Nilsson-Öst ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Antimicrobial Susceptibility ◽  
Clinical Mastitis

Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows

2002 ◽  
Vol 19 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Véronique Guérin-Faublée ◽  
Florence Tardy ◽  
Clarisse Bouveron ◽  
Gérard Carret
Keyword(s):  
Dairy Cows ◽  
Antimicrobial Susceptibility ◽  
Clinical Mastitis ◽  
Streptococcus Species
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