scholarly journals Neuroprotective effects of Camellia nitidissima Chi leaf extract in hydrogen peroxide‐treated human neuroblastoma cells and its molecule mechanisms

2020 ◽  
Vol 8 (9) ◽  
pp. 4782-4793
Author(s):  
Lei An ◽  
Wei Zhang ◽  
Guowei Ma ◽  
Ke Wang ◽  
Yufei Ji ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Leaf Extract ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Neuroprotective Effects ◽  
Human Neuroblastoma ◽  
Camellia Nitidissima

scholarly journals The antioxidant xanthorrhizol prevents amyloid-β-induced oxidative modification and inactivation of neprilysin

2018 ◽  
Vol 38 (1) ◽  
Author(s):  
Chol Seung Lim ◽  
Jung-Soo Han
Keyword(s):  
Therapeutic Potential ◽  
Neuroblastoma Cells ◽  
Amyloid Β ◽  
Enzymatic Activities ◽  
Oxidative Modification ◽  
Amyloid Β Peptide ◽  
Oxygen Species ◽  
Human Neuroblastoma Cells ◽  
Neuroprotective Effects ◽  
Human Neuroblastoma

Activity of neprilysin (NEP), the major protease which cleaves amyloid-β peptide (Aβ), is reportedly reduced in the brains of patients with Alzheimer’s disease (AD). Accumulation of Aβ generates reactive oxygen species (ROS) such as 4-hydroxynonenal (HNE), and then reduces activities of Aβ-degrading enzymes including NEP. Xanthorrhizol (Xan), a natural sesquiterpenoid, has been reported to possess antioxidant and anti-inflammatory properties. The present study examined the effects of Xan on HNE- or oligomeric Aβ42-induced oxidative modification of NEP protein. Xan was added to the HNE- or oligomeric Aβ42-treated SK-N-SH human neuroblastoma cells and then levels, oxidative modification and enzymatic activities of NEP protein were measured. Increased HNE levels on NEP proteins and reduced enzymatic activities of NEP were observed in the HNE- or oligomeric Aβ42-treated cells. Xan reduced HNE levels on NEP proteins and preserved enzymatic activities of NEP in HNE- or oligomeric Aβ42-treated cells. Xan reduced Aβ42 accumulation and protected neurones against oligomeric Aβ42-induced neurotoxicity through preservation of NEP activities. These findings indicate that Xan possesses therapeutic potential for the treatment of neurodegenerative diseases, including AD, and suggest a potential mechanism for the neuroprotective effects of antioxidants for the prevention of AD.

Lichen Based Synthesis of Zinc Oxide Nanoparticles and Evaluation of its Neurotoxic Effects on Human Neuroblastoma Cells

2019 ◽  
Vol 59 ◽  
pp. 15-24
Author(s):  
Fatih Doğan Koca ◽  
Gökhan Ünal ◽  
Mehmet Gökhan Halici
Keyword(s):  
Hydrogen Peroxide ◽  
Zinc Oxide Nanoparticles ◽  
Neuroblastoma Cells ◽  
Oxide Nanoparticles ◽  
Human Neuroblastoma Cells ◽  
Zno Nps ◽  
Human Neuroblastoma ◽  
Neurotoxic Effects ◽  
Higher Doses ◽  
Scanning Electron

Nanoparticles, smaller than 100 nm are synthesized by chemical and physical methods. Biological synthesis of nanoparticles is very popular in science recently. The aim of the study is green synthesis of zinc oxide nanoparticles (ZnO NPs) using the lichen extract (Ramalina fraxinea) and investigating the cytotoxic effects of ZnO NPs on human neuroblastoma cells (SHSY-5Y). Despite the widespread use of ZnO NPs, a limited number of studies have investigated the neurobiological effects of ZnO NP. Therefore, we tested the neurotoxic effect of green synthesized ZnO NPs administration and its neuroprotective effect against hydrogen peroxide-induced cell damage on SH-SY5Y human neuroblastoma cell line. The absorbance peak of the ZnO NPs was detected by UV–visible spectroscopy (UV-Vis) at 330 nm. The average diameter of ZnO NPs was measured as about 21 nm by Scanning Electron Microscope (SEM) and Field Emission Scanning Electron Microscope (FE-SEM) images. According to X-ray Diffraction (XRD) diagram, ZnO NPs were hexagonal in structure. The peaks observed in the Fourier Transform Infrared (FT-IR) test showed functional groups in the structure of the nanoparticles. According to our results, ZnO NPs may have beneficial effects at the low concentrations while neurotoxic effects at the higher doses in SH-SY5Y. In addition, we indicate that hydrogen peroxide-induced cell death could not reverse by ZnO NPs and its higher doses potentiated the neurodegenerative effect of hydrogen peroxide. In conclusion, here we report that ZnO NPs, widely used in various products, may have beneficial or harmful effects in a dose-dependent manner and play a role in neuropsychiatric disease, especially neurodegenerative diseases. This is the first study dealing with neurotoxicity on SHSY-5Y of Ramalina fraxinea extract based ZnO NPs.

scholarly journals Maltol Inhibits Apoptosis of Human Neuroblastoma Cells Induced by Hydrogen Peroxide

BMB Reports ◽  
2006 ◽  
Vol 39 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Yang Yang ◽  
Jian Wang ◽  
Caimin Xu ◽  
Huazhen Pan ◽  
Zinan Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma
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