scholarly journals The interaction capabilities of phytoconstituents of ethanolic seed extract of cumin ( Cuminum cyminum L.) with HMG‐CoA reductase to subside the hypercholesterolemia: A mechanistic approach

2021 ◽  
Author(s):  
Harshlata Chouhan ◽  
Ashok Purohit ◽  
Heera Ram ◽  
Suman Chowdhury ◽  
Priya Kashyap ◽  
...  
Keyword(s):  
Seed Extract ◽  
Hmg Coa Reductase ◽  
Cuminum Cyminum ◽  
Mechanistic Approach ◽  
Coa Reductase

scholarly journals Ameliorations in the Biomarker Indices of Dyslipidemia and Atherosclerotic Plaque by the Inhibition of HMG – CoA Reductase and Antioxidant Potential of Phytoconstituents of an Aqueous Seed Extract of Acacia Senegal (L.) Willd in Rabbits

2021 ◽  
Author(s):  
Jaykaran Charan ◽  
Priyanka Riyad ◽  
Heera Ram ◽  
Ashok Purohit ◽  
Sneha Ambwani ◽  
...  
Keyword(s):  
Atherosclerotic Plaque ◽  
In Silico ◽  
Risk Index ◽  
Seed Extract ◽  
Atherogenic Index ◽  
Acacia Senegal ◽  
Hmg Coa Reductase ◽  
Coa Reductase

Abstract Background: The HMG-CoA inhibitor are used to control adverse cardiovascular event caused by Hypercholesterolemia and dyslipidaemia. The current study was aimed to evaluate the ability of phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd to inhibit HMG-CoA reductase and regress the formation of atherosclerotic plaque. Methods: The chemical fingerprinting of the test extract was assessed by LC-MS. Consequently, the assessments of in-vitro, in-vivo, and in-silico were performed by following the standard methods.Results: The in-vitro assessment of the test extract revealed 74.1 % inhibition potential of HMG-CoA reductase. In-vivo evaluations of the test extract indicated that treated hypercholesterolemic rabbits exhibited a significant (𝑃 ≤ 0.001) ameliorations in the biomarker indices of the dyslipidaemia, such as the atherogenic index, Castelli risk index (I&II), atherogenic coefficient along with lipid profile. Concomitantly, significant reductions were observed in the atherosclerotic plaque area and antioxidants. The in-silico study of molecular docking shown interactions capabilities of key phytoconstituents of the test extract with target protein of HMG-CoA reductase which further validated by the molecular dynamics through potentail energy, NPT, NVT, RSMD and others. Subsequently, the ADMET analysis shown ideal druggability. Conclusion: The results indicate that phytoconstituents of an aqueous seed extract of Acacia senegal (L.) Willd. could inhibit HMG-CoA reductase and improve the levels of antioxidants activity that may reduce symptoms associated with hypercholesterolemia.

scholarly journals HMG-CoA Reductase Inhibitory Activity of Gnetum gnemon Seed Extract and Identification of Potential Inhibitors for Lowering Cholesterol Level

2017 ◽  
Vol 9 (4) ◽  
pp. 559-565 ◽  
Author(s):  
Kholid Abdul Hafidz ◽  
Nuraini Puspitasari ◽  
Azminah A ◽  
Arry Yanuar ◽  
Yuditya Artha ◽  
...  
Keyword(s):  
Cholesterol Level ◽  
Inhibitory Activity ◽  
Seed Extract ◽  
Hmg Coa Reductase ◽  
Gnetum Gnemon ◽  
Coa Reductase ◽  
Potential Inhibitors

scholarly journals Consortia of bioactives in supercritical carbon dioxide extracts of mustard and small cardamom seeds lower serum cholesterol levels in rats: new leads for hypocholesterolaemic supplements from spices

2019 ◽  
Vol 8 ◽  
Author(s):  
Soumi Chakraborty ◽  
Kaninika Paul ◽  
Priyanka Mallick ◽  
Shrabani Pradhan ◽  
Koushik Das ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Antioxidant Activities ◽  
Reductase Activity ◽  
Seed Extract ◽  
Green Technology ◽  
Albino Rats ◽  
Resonance Spectroscopy ◽  
Hmg Coa Reductase ◽  
Cholesterol Levels ◽  
Coa Reductase

AbstractMelatonin-rich and 1,8-cineole-rich extracts have been successfully obtained from yellow mustard (YM) and small cardamom (SC) seeds, respectively, employing green technology of supercritical CO2 (SC-CO2) extraction. Chemical profiling confirmed the presence of melatonin and 1,8-cineole and co-extractants in the respective extracts. Electron paramagnetic resonance spectroscopy attested strong antioxidant activities of the extracts foregoing pan-assay interference compounds involved in spectroscopic analysis. These extracts also exhibited synergistic efficacies greater than unity confirming antioxidant synergy among the co-extracted bioactives therein. To ascertain hypocholesterolaemic efficacies, these extracts were co-administered orally with Triton X (at the pre-optimised dose of 175 mg/kg body weight (BW)) to Wistar albino rats at doses of 550, 175 and 55 mg/kg BW. Serum total cholesterol levels in the rats were monitored on days 3, 7, 15 and 21. On day 21, total cholesterol level reduced appreciably by 49·44 % in rats treated with YM seed extract and by 48·95 % in rats treated with SC seed extract, comparable with atorvastatin-administered rats (51·09 %). Either extract demonstrated inhibitory effects on hepatic 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase activity. A molecular docking exercise identified specific compounds in the extracts which possessed binding affinities comparable with therapeutically used HMG-CoA reductase inhibitors. In silico and in vivo studies concertedly concluded that the consortium of bioactive components in the extracts cannot be considered as invalid metabolic panaceas and therefore these ‘green’ extracts could be safely subjected to clinical studies as preventive biotherapeutics for hypercholesterolaemia. These extracts could be consumed per se as hypocholesterolaemic supplements or could be ingredients of new spice-based therapeutic foods.

Reduction of HMG-CoA-reductase leads to improved cardiac function in doxorubicin-induced cardiomyopathy in mice

2008 ◽  
Vol 7 ◽  
pp. 202-203
Author(s):  
A RIAD ◽  
S BIEN ◽  
F ESCHER ◽  
D WESTERMANN ◽  
U LANDMESSER ◽  
...  
Keyword(s):  
Cardiac Function ◽  
Hmg Coa Reductase ◽  
Coa Reductase
Sign in / Sign up

Export Citation Format

Share Document