Characteristics of an isomenthone-rich somaclonal mutant isolated in a geraniol-rich rose-scented geranium accession ofPelargonium graveolens

2001 ◽  
Vol 16 (5) ◽  
pp. 319-324 ◽  
Author(s):  
Ritika Gupta ◽  
G. R. Mallavarapu ◽  
S. Banerjee ◽  
Sushil Kumar
Keyword(s):  
Somaclonal Mutant

Genetic and molecular analysis of a purple sheath somaclonal mutant in japonica rice

2011 ◽  
Vol 30 (5) ◽  
pp. 901-911 ◽  
Author(s):  
Dongying Gao ◽  
Bing He ◽  
Yihong Zhou ◽  
Lihua Sun
Keyword(s):  
Molecular Analysis ◽  
Japonica Rice ◽  
Somaclonal Mutant

Characterization of the S-Locus Region of Almond (Prunus dulcis): Analysis of a Somaclonal Mutant and a Cosmid Contig for an S Haplotype

Genetics ◽  
2001 ◽  
Vol 158 (1) ◽  
pp. 379-386
Author(s):  
Koichiro Ushijima ◽  
Hidenori Sassa ◽  
Mihoko Tamura ◽  
Makoto Kusaba ◽  
Ryutaro Tao ◽  
...  
Keyword(s):  
Southern Blot ◽  
Prunus Dulcis ◽  
S Locus ◽  
Extra Copy ◽  
Physical Limit ◽  
Cosmid Contig ◽  
Almond Cultivar ◽  
Insight Into ◽  
Somaclonal Mutant

Abstract Almond has a self-incompatibility system that is controlled by an S locus consisting of the S-RNase gene and an unidentified “pollen S gene.” An almond cultivar “Jeffries,” a somaclonal mutant of “Nonpareil” (ScSd), has a dysfunctional Sc haplotype both in pistil and pollen. Immunoblot and genomic Southern blot analyses detected no Sc haplotype-specific signal in Jeffries. Southern blot showed that Jeffries has an extra copy of the Sd haplotype. These results indicate that at least two mutations had occurred to generate Jeffries: (1) deletion of the Sc haplotype and (2) duplication of the Sd haplotype. To analyze the extent of the deletion in Jeffries and gain insight into the physical limit of the S locus region, ∼200 kbp of a cosmid contig for the Sc haplotype was constructed. Genomic Southern blot analyses showed that the deletion in Jeffries extends beyond the region covered by the contig. Most cosmid end probes, except those near the Sc-RNase gene, cross-hybridized with DNA fragments from different S haplotypes. This suggests that regions away from the Sc-RNase gene can recombine between different S haplotypes, implying that the cosmid contig extends to the borders of the S locus.

Penetrance, expressivity and inheritance of axillary branching in somaclonal mutant of sorghum (Sorghum bicolor L.)

Euphytica ◽  
2014 ◽  
Vol 196 (3) ◽  
pp. 449-457 ◽  
Author(s):  
Shobha Immadi ◽  
Shreekant Patil ◽  
Manjula Maralappanavar ◽  
Gowramma Sajjanar
Keyword(s):  
Sorghum Bicolor ◽  
Axillary Branching ◽  
Sorghum Bicolor L ◽  
Somaclonal Mutant

Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase

1986 ◽  
Vol 202 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Richard I. S. Brettell ◽  
Elizabeth S. Dennis ◽  
William R. Scowcroft ◽  
W. James Peacock
Keyword(s):  
Alcohol Dehydrogenase ◽  
Molecular Analysis ◽  
Somaclonal Mutant

scholarly journals CHARACTERIZATION OF PAPAYAS WITH GENETICALLY ENGINEERED VIRUS RESISTANCE

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 660a-660
Author(s):  
Lius Suwenza ◽  
Richard Manshardt
Keyword(s):  
Coat Protein ◽  
Virus Resistance ◽  
Molecular Level ◽  
Microprojectile Bombardment ◽  
Genetically Engineered ◽  
Flower Morphology ◽  
Papaya Ringspot Virus ◽  
Normal Fertility ◽  
Somaclonal Mutant

Nine transgenic papaya clones, produced previously by microprojectile bombardment, are being characterized for frequency of somaclonal variation. Five clones have proven to be hermaphrodite. Four of these appear to have normal fertility, while the fifth has drastically reduced pollen fertility, averaging about 15% stainability with acetocarmine. Four other clones are pistillate and appear to have normal fertility, with one exception which has been demonstrated to be tetraploid (2n=36 chromosomes). One of twelve plants in a pistillate clone was a somaclonal mutant showing altered leaf and flower morphology. The transgenic clones and their sexual progenies are also being evaluated at the molecular level for expression and segregation of npt, gus, and the coat protein (CP) of papaya ringspot virus (PRV), as well as for PRV resistance.

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