Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss)

2002 ◽  
Vol 21 (3) ◽  
pp. 557-560 ◽  
Author(s):  
Barbara Shayne Washburn ◽  
Jason J. Moreland ◽  
Ann M. Slaughter ◽  
Inge Werner ◽  
David E. Hinton ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Heat Shock Protein Expression

EFFECTS OF HANDLING ON HEAT SHOCK PROTEIN EXPRESSION IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

2002 ◽  
Vol 21 (3) ◽  
pp. 557 ◽  
Author(s):  
Barbara Shayne Washburn ◽  
Jason J. Moreland ◽  
Ann M. Slaughter ◽  
Inge Werner ◽  
David E. Hinton ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Heat Shock Protein Expression

Heat Shock Protein Regulation in Rainbow Trout,Oncorhynchus mykiss, is Altered by Dietary Soybean Meal Inclusion and Anti-Phopholipase A2Antibody

2013 ◽  
Vol 44 (5) ◽  
pp. 655-668 ◽  
Author(s):  
Wendy M. Sealey ◽  
Frederic T. Barrows ◽  
Charles E. Smith ◽  
Jurij M. Wacyk ◽  
Madison S. Powell ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Oncorhynchus Mykiss ◽  
Soybean Meal ◽  
Protein Regulation ◽  
Rainbow Trout Oncorhynchus Mykiss

Effects of heat stress on biochemical parameters and heat shock protein family A (Hsp70) member 5 (HSPA5) mRNA expression in rainbow trout (Oncorhynchus mykiss)

2018 ◽  
Vol 69 (11) ◽  
pp. 1674 ◽  
Author(s):  
Binpeng Xia ◽  
Zhe Liu ◽  
Yanjing Zhou ◽  
Yongjie Wang ◽  
Jinqiang Huang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Oncorhynchus Mykiss ◽  
Mrna Expression ◽  
Biochemical Parameters ◽  
Cold Water ◽  
Protein Family ◽  
Rainbow Trout Oncorhynchus Mykiss

Rainbow trout (Oncorhynchus mykiss) is a cold-water species of salmonid, and high temperatures are a significant threat to its aquaculture. In order to understand the degree of the heat stress response and the mechanisms involved, full-sibling inbred O. mykiss individuals were sampled at 18, 21, 23, 24, 25 and 26°C to investigate changes in some serum biochemical parameters, as well as in the mRNA expression of heat shock protein family A (Hsp70) member 5 (HSPA5; also known as glucose regulated protein 78 (GRP78)) in different tissues (liver, mid-kidney, heart, spleen and brain). At 21°C, there was a significant increase in the spleen macrophage respiratory burst and a significant decrease in superoxide dismutase activity compared with 18°C (P<0.05). Malondialdehyde peaked at 23°C, whereas alanine transaminase and aspartate aminotransferase activity were both twofold higher at 25 and 26°C compared with that at 18°C. The Ca2+, Mg2+, PO43– and glucose (Glu) content of serum declined significantly at 21°C relative to 18°C (P<0.05). The expression of HSPA5 mRNA responded in a temperature- and tissue-specific manner to heat stress. Except for in the spleen, HSPA5 mRNA expression was significantly higher in all tissues at 25 and 26°C than that at 18°C (P<0.05). These results indicate that heat stress causes oxidative damage, decreases the Ca2+, Mg2+, PO43– and Glu content of serum and induces HSPA5 mRNA expression.

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