Whole blood concentrations of organochlorines as a dose metric for studies of the glaucous gull (Larus hyperboreus)

2001 ◽  
Vol 20 (5) ◽  
pp. 1046-1052 ◽  
Author(s):  
Jan O. Bustnes ◽  
Janneche U. Skaare ◽  
Kjell E. Erikstad ◽  
Vidar Bakken ◽  
Fritjof Mehlum
Keyword(s):  
Whole Blood ◽  
Blood Concentrations ◽  
Larus Hyperboreus ◽  
Glaucous Gull ◽  
Dose Metric

WHOLE BLOOD CONCENTRATIONS OF ORGANOCHLORINES AS A DOSE METRIC FOR STUDIES OF THE GLAUCOUS GULL (LARUS HYPERBOREUS)

2001 ◽  
Vol 20 (5) ◽  
pp. 1046 ◽  
Author(s):  
Jan O. Bustnes ◽  
Janneche U. Skaare ◽  
Kjell E. Erikstad ◽  
Vidar Bakken ◽  
Fritjof Mehlum
Keyword(s):  
Whole Blood ◽  
Blood Concentrations ◽  
Larus Hyperboreus ◽  
Glaucous Gull ◽  
Dose Metric

Glaucous Gull (Larus hyperboreus)

2012 ◽  
Author(s):  
Emily Weiser ◽  
H. Grant Gilchrist
Keyword(s):  
Larus Hyperboreus ◽  
Glaucous Gull

Glaucous Gull (Larus hyperboreus)

2012 ◽  
Author(s):  
Emily Weiser ◽  
H. Grant Gilchrist
Keyword(s):  
Larus Hyperboreus ◽  
Glaucous Gull

First Documentation of Plastic Ingestion in the Arctic Glaucous Gull (Larus Hyperboreus)

2021 ◽  
Author(s):  
Stine Charlotte Benjaminsen ◽  
Sophie Bourgeon ◽  
Dorte Herzke ◽  
France Collard ◽  
Amalie Ask ◽  
...  
Keyword(s):  
The Arctic ◽  
Larus Hyperboreus ◽  
Glaucous Gull

scholarly journals Liquid Chromatography Tandem Mass Spectrometric Analytical Method for Study of Colchicine in Rats Given Low Doses

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2007
Author(s):  
Hamdah M. Al Nebaihi ◽  
Tyson S. Le ◽  
Neal M. Davies ◽  
Dion R. Brocks
Keyword(s):  
Whole Blood ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Rat Plasma ◽  
Low Doses ◽  
Blood Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
Sensitivity And Selectivity ◽  
Tandem Mass Spectrometric ◽  
Lower Limits

A selective and sensitive assay was developed for colchicine in rat specimens. Colchicine and its deuterated analog (as internal standard, IS) were extracted from rat specimens (minimal 0.1 mL plasma, whole blood, or urine) using liquid-liquid extraction with n-hexane:dichloromethane:isopropanol. The mobile phase (formic acid: ammonium acetate: methanol) was pumped with uniform flow through an octadecylsilane analytical column. Detection was carried out by electrospray positive ionization in the multiple-reaction monitoring mode. The assay (total run time <3 min) had excellent linearity over a wide (400–800-fold) concentration range. The mean absolute recovery was >96.8%. The intra- and inter-day coefficients of variation were ˂15%, with lower limits of quantitation of 0.5 ng/mL in 0.1 mL of rat plasma. The method also provided the same lower limits of quantitation in urine and whole blood with 0.1 mL volumes, and 0.1 ng/mL using 0.5 mL of rat plasma. The blood-to-plasma ratio was >1. Rats had measurable colchicine blood concentrations for at least 24 h after intravenous doses of 0.1 mg/kg. The method possessed suitable measures of sensitivity and selectivity for detecting colchicine in several specimen types in rats given low doses.

Δ9-THC, 11-OH-Δ9-THC and Δ9-THCCOOH plasma or serum to whole blood concentrations distribution ratios in blood samples taken from living and dead people

2001 ◽  
Vol 123 (2-3) ◽  
pp. 159-164 ◽  
Author(s):  
Christian Giroud ◽  
Annick Ménétrey ◽  
Marc Augsburger ◽  
Thierry Buclin ◽  
Pablo Sanchez-Mazas ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Concentrations ◽  
Blood Samples

Radioreceptor Assay for Quantifying FK-506 Immunosuppressant in Whole Blood

1992 ◽  
Vol 38 (7) ◽  
pp. 1307-1310 ◽  
Author(s):  
J N Murthy ◽  
Y Chen ◽  
V S Warty ◽  
R Venkataramanan ◽  
J G Donnelly ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Whole Blood ◽  
Binding Protein ◽  
Radioreceptor Assay ◽  
Fk 506 ◽  
Blood Concentrations ◽  
Chromatographic Columns ◽  
Analytical Recovery

Abstract We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [3H]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chromatographic columns. We compared the results of this method with those of an enzyme immunoassay that uses a monoclonal antibody: r = 0.97, Sy/x = 0.039. Between-day precisions (CV) at FK-506 concentrations of 8 and 17 micrograms/L were 9.2% and 8.2%, respectively. Within-run precisions were 5.9%, 8.1%, and 9.4%, respectively, at 4, 8, and 15 micrograms/L. Analytical recovery, evaluated at 5, 10, 15, 20, and 25 micrograms/L for FK-506 added to whole blood, ranged from 98% to 103%. The assay can reliably quantify FK-506 blood concentrations between 1.0 and 25 micrograms/L.

Glaucous Gull (Larus hyperboreus) at Philadelphia

The Auk ◽  
1918 ◽  
Vol 35 (2) ◽  
pp. 221-221
Author(s):  
Witmer Stone
Keyword(s):  
Larus Hyperboreus ◽  
Glaucous Gull

scholarly journals Assay of prostate-specific antigen from whole blood spotted on filter paper and application to prostate cancer screening

1996 ◽  
Vol 42 (4) ◽  
pp. 536-544 ◽  
Author(s):  
B R Hoffman ◽  
H Yu ◽  
E P Diamandis
Keyword(s):  
Prostate Cancer ◽  
Prostate Specific Antigen ◽  
Filter Paper ◽  
Whole Blood ◽  
Large Scale ◽  
Prostate Cancer Screening ◽  
Specific Antigen ◽  
Blood Concentrations ◽  
Hot Climate ◽  
Plasma Fraction

Abstract We report the measure of prostate-specific antigen (PSA) from extracts of blood dried on filter paper. Five 3-mm (diameter) paper discs containing approximately 25 microL of dried whole blood were punched from the filter paper and extracted with 500 microL of buffer. Recovery of PSA was &gt; 92%. Imprecision of the filter paper procedure was &lt;10% when corresponding whole-blood concentrations were &gt;0.35 micrograms/L. PSA recovery was unaffected whether blood was applied to the filter as one 85-microL aliquots, two 43-microL aliquots, or three 28-microL aliquots. PSA is contained in the plasma fraction. Variation in hematocrit from 0.61 to 0.31 caused &lt;+/-10% change in filter paper PSA. Regression analysis showed: filter paper PSA = 0.86 whole-blood PSA - 0.02; Sy/x = 0.44. Men (153) without prostate cancer gave a 95th percentile of 4.8 micrograms /L. PSA in filter paper dried blood was stable for &gt;1 month at -20 to 37 degrees C and showed no loss of recovery after being mailed to a hot climate. We conclude that the filter paper procedure can reliably distinguish normal from increased concentrations of PSA and that it could facilitate screening to detect occult prostate cancer in large-scale mail-in programs to centralized laboratories.

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