An in vitro assessment of the toxicity of 2,4-dichloroaniline using rainbow trout primary epidermal cell cultures

2000 ◽  
Vol 19 (8) ◽  
pp. 2093-2099 ◽  
Author(s):  
Michael Kilemade ◽  
Carmel Mothersill
Keyword(s):  
Rainbow Trout ◽  
Epidermal Cell ◽  
Cell Cultures ◽  
In Vitro Assessment

scholarly journals In Vitro Assessment of Anticoccidials: Methods and Molecules

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1962
Author(s):  
Martina Felici ◽  
Benedetta Tugnoli ◽  
Andrea Piva ◽  
Ester Grilli
Keyword(s):  
Cell Cultures ◽  
Mode Of Action ◽  
Economic Losses ◽  
Poultry Industry ◽  
Protozoan Parasites ◽  
In Ovo ◽  
Eimeria Spp ◽  
In Vitro Assessment ◽  
Avian Coccidiosis

Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by Eimeria spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.

scholarly journals Replication of Infectious Pancreatic Necrosis Virus in Different Cell Lines and in Rainbow Trout (Oncorhynchus Mykiss) Fingerlings

2014 ◽  
Vol 22 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Natalija Matvienko ◽  
Yury Rud ◽  
Leonid Buchatsky
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cell Cultures ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Disease Symptoms ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Abstract The results of a study of Infectious Pancreatic Necrosis Virus (IPNV) isolated in natural reservoirs in Ukraine are presented. The pathogenicity of isolates was investigated in vitro on cell cultures and in vivo on rainbow trout, Oncorhynchus mykiss (Walbaum), fingerlings. Experimental indications were that the Ukrainian IPNV isolates have affinity with reference European strains. During the reproduction of these isolates in cell cultures of FHM (fat head minnow), RTG-2 (rainbow trout gonads), and BF-2 (bluegill caudal peduncle), complicated degenerative changes were visible that finally led to the full destruction of cell monolayers. The experimental infection of rainbow trout fingerlings resulted in typical disease symptoms that were systemic. However, obvious evidence of viral infection was noted in single individuals only, and the majority of experimental fish died without visible disease symptoms. During the study of physicochemical properties, it was noted that Ukrainian isolates completely lost their infectivity with chloroform treatment and heating to 60°C. This proved that IPNV isolates are resistant to Ion concentrations in the range of pH 3.0 to 12.0.

Rainbow trout primary epidermal cell proliferation as an indicator of aquatic toxicity: an in vitro/in vivo exposure comparison

2002 ◽  
Vol 60 (1-2) ◽  
pp. 43-59 ◽  
Author(s):  
Michael Kilemade ◽  
Maria Lyons-Alcantara ◽  
Tina Rose ◽  
Richard Fitzgerald ◽  
Carmel Mothersill
Keyword(s):  
Cell Proliferation ◽  
Rainbow Trout ◽  
Epidermal Cell ◽  
Aquatic Toxicity ◽  
Epidermal Cell Proliferation
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