Use of fish gill cells in culture to evaluate the cytotoxicity and photocytotoxicity of intact and photomodified creosote

1999 ◽  
Vol 18 (6) ◽  
pp. 1277-1288 ◽  
Author(s):  
Kristin Schirmer ◽  
Jo-Anne S. Herbrick ◽  
Bruce M. Greenberg ◽  
D. George Dixon ◽  
Niels C. Bols
Keyword(s):  
Fish Gill ◽  
Cells In Culture ◽  
Gill Cells

Swelling-activated transport of taurine in cultured gill cells of sea bass: physiological adaptation and pavement cell plasticity

2009 ◽  
Vol 296 (4) ◽  
pp. R1149-R1160 ◽  
Author(s):  
Martine Avella ◽  
Olivier Ducoudret ◽  
Didier F. Pisani ◽  
Philippe Poujeol
Keyword(s):  
Sea Bass ◽  
Physiological Adaptation ◽  
Dimensional Structure ◽  
Taurine Transport ◽  
Hypotonic Shock ◽  
Cells In Culture ◽  
Membrane Stretch ◽  
Gill Cells ◽  
Fold Stimulation ◽  
Stimulation Of

We have investigated volume-activated taurine transport and ultrastructural swelling response of sea bass gill cells in culture, assuming that euryhaline fish may have developed particularly efficient mechanisms of salinity adaptation. In vivo, when sea basses were progressively transferred from seawater to freshwater, we noticed a decrease in blood osmotic pressure. When gill cells in culture were subjected to 30% hypotonic shock, we observed a five-fold stimulation of [3H]taurine efflux. This transport was reduced by various anion channel inhibitors with the following efficiency: 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) > niflumic acid > DIDS = diphenylamine-2-carboxylic acid. With polarized gill cells in culture, the hypotonic shock produced a five-fold stimulation of apical taurine transport, whereas basolateral exit was 25 times higher. Experiments using ionomycin, thapsigargin, BAPTA-AM, or removal of extracellular calcium suggested that taurine transport was regulated by external calcium. The inhibitory effects of lanthanum and streptomycin support Ca2+ entry through mechanosensitive Ca2+ channels. Branchial cells also showed hypotonically activated anionic currents sensitive to DIDS and NPPB. Similar pharmacology and time course suggested the potential existence of a common pathway for osmosensitive taurine and Cl− efflux through volume-sensitive organic osmolyte and anion channels. A three-dimensional structure study revealed that respiratory gill cells began to swell only 15 s after hypoosmotic shock. Apical microridges showed membrane outfoldings: the cell surface became smoother with a progressive disappearance of ridges. Therefore, osmotic swelling may not actually induce membrane stretch per se, inasmuch as the microridges may provide a reserve of surface area. This work demonstrates mechanisms of functional and morphological plasticity of branchial cells during osmotic stress.

Transcriptomic analysis of metal responses in cultured fish gill cells

2007 ◽  
Vol 146 (4) ◽  
pp. S70 ◽  
Author(s):  
C. Hogstrand ◽  
G. Feeney ◽  
P. Walker ◽  
D. Zheng ◽  
P. Kille
Keyword(s):  
Transcriptomic Analysis ◽  
Fish Gill ◽  
Cultured Fish ◽  
Gill Cells

Surface complexation of aluminum on isolated fish gill cells

1993 ◽  
Vol 27 (6) ◽  
pp. 1132-1138 ◽  
Author(s):  
Kevin J. Wilkinson ◽  
Paul M. Bertsch ◽  
Charles H. Jagoe ◽  
Peter G. C. Campbell
Keyword(s):  
Surface Complexation ◽  
Fish Gill ◽  
Gill Cells

A fish gill system for quantifying the ultrastructural effects of environmental stressors: methylmercury, Kepone®, and heat shock

1995 ◽  
Vol 52 (6) ◽  
pp. 1165-1182 ◽  
Author(s):  
Jon Mallatt ◽  
Steven J. Lampa ◽  
J. Franklin Bailey ◽  
Marc A. Evans ◽  
Steve Brumbaugh
Keyword(s):  
Heat Shock ◽  
Environmental Stressors ◽  
Cell Type ◽  
Fish Gill ◽  
Specific Effects ◽  
Transmission Electron ◽  
Sea Lampreys ◽  
Gill Cells ◽  
Mitochondrial Volume ◽  
Histopathological Studies

In previous histopathological studies, fish gills have been used to study nonspecific responses of tissue to injury; however, such studies have revealed little about the specific effects of different toxicants on cells. Here, we used transmission electron microscopy plus a morphometric technique (stereology) to determine how several types of gill cells are specifically altered by three stressors: methylmercury, heat shock, and the insecticide Kepone® (chlordecone). Larval sea lampreys, Petromyzon marinus, were exposed to various concentrations of waterborne stressor (ranging from the incipient lethal concentrations to a 24-h LC50) for different periods (half a day or 3 weeks). Methylmercury increased the volumes of gill epithelial cells and their nuclei and mitochondria, presumably by inducing ambient water to leak into these structures. Heat shock, by contrast, decreased the volumes of most cells and their organelles, presumably by causing cells to shed pieces of cytoplasm. Kepone had almost no effect, but the few changes that were recorded (increased mitochondrial volume in one cell type) are consistent with known actions of Kepone® (alteration of mitochondrial respiration). The results prove that a fish gill can be used to probe the specific actions of different toxicants and pollutants.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

The nuclear 3,5,3'-triiodothyronine receptor in human leukaemic cell lines

1984 ◽  
Vol 105 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Juan Bernal ◽  
Leif C. Andersson
Keyword(s):  
Growth Hormone ◽  
Cell Line ◽  
Cell Lines ◽  
Rat Liver ◽  
Association Constant ◽  
Erythroid Differentiation ◽  
Leukaemic Cell ◽  
Cells In Culture ◽  
Gh Cells ◽  
High Concentrations

Abstract. The 3,5,3'-triiodothyronine (T3) receptor has been studied in a series of continuously growing human leukaemic cell lines. High concentrations of receptor were found in the erythroblastoid cell line K-562. T3 was bound to the nuclei of these cells with an association constant of 3.4 × 109 m−1, and capacity 104 fmol/100 μg DNA, or 8700 molecules/nucleus. This capacity is comparable to that of rat liver or growth hormone producing cells (GH cells) in culture, and suggests that the K-562 cell line could be a useful model for the study of T3 action on erythroid differentiation.

Increased matrix gene expression by glucose in rat neural connective tissue cells in culture

Diabetes ◽  
1991 ◽  
Vol 40 (5) ◽  
pp. 605-611 ◽  
Author(s):  
P. Muona ◽  
J. Peltonen ◽  
S. Jaakkola ◽  
J. Uitto
Keyword(s):  
Gene Expression ◽  
Connective Tissue ◽  
Cells In Culture ◽  
Matrix Gene ◽  
Tissue Cells
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