Comparison of in vitro submitochondrial particle and Microtox® assays for determining the toxicity of organotin compounds

1998 ◽  
Vol 17 (6) ◽  
pp. 1005-1012 ◽  
Author(s):  
Emanuele Argese ◽  
Cinzia Bettiol ◽  
Annamaria Volpi Ghirardini ◽  
Matteo Fasolo ◽  
Gianumberto Giurin ◽  
...  
Keyword(s):  
Organotin Compounds ◽  
Submitochondrial Particle

Cytotoxicity in vitro of organotin compounds to fish hepatoma cells PLHC-1 (Poeciliopsis lucida)

1995 ◽  
Vol 32 (2-3) ◽  
pp. 143-160 ◽  
Author(s):  
Beat J. Brüschweiler ◽  
Friedrich E. Würgler ◽  
Karl Fent
Keyword(s):  
Hepatoma Cells ◽  
Organotin Compounds ◽  
Poeciliopsis Lucida

Profile of toxic response to sediments using whole-animal and in vitro submitochondrial particle (SMP) assays

1996 ◽  
Vol 15 (3) ◽  
pp. 319-324 ◽  
Author(s):  
Alan D. Bettermann ◽  
Jonathan C. Dorofi ◽  
James M. Lazorchak
Keyword(s):  
Toxic Response ◽  
Submitochondrial Particle

Organotin compounds decrease in vitro survival, proliferation and differentiation of normal human B lymphocytes

1999 ◽  
Vol 18 (10) ◽  
pp. 619-624 ◽  
Author(s):  
A De Santiago ◽  
M Aguilar-Santelises
Keyword(s):  
B Cells ◽  
In Vitro Culture ◽  
B Lymphocytes ◽  
Organometallic Compounds ◽  
Organotin Compounds ◽  
Human B Cells ◽  
Cell Immunity ◽  
Staphylococcus Aureus Cowan ◽  
Contaminated Food

Organotin compounds (OTC) are organometallic compounds with vast industrial and agriculture applications that give rise to ubiquitous environmental contamination. OTC are immunotoxic, but most studies have been performed in rodents and almost exclusively focused on T cell immunity. Humans can be exposed to OTC by inhalation, absorption, and consumption of contaminated food and water. To analyse the effects of OTC in human immune tissue, we isolated B cells from tonsils and exposed them to five OTC at various concentrations, during in vitro culture. Non-stimulated B cells were killed by 100 nM of all tested OTC after 8 h in vitro culture, under sub-optimal conditions, except TET. OTC also decreased the proliferation of tonsillar B lymphocytes stimulated with Staphylococcus aureus Cowan 1 (SAC) and IL-2, when present at 100 nM and higher concentrations. IgM secretion was reduced in stimulated cell cultures exposed to 100 nM dibutyltin chloride (DBT). Accordingly, increased phosphatidylserine exposure demonstrated that 100 nM TPT and DBT induced B cells to die by apoptosis. These data indicate that human B cells are diminished in their capacity to survive, proliferate and differentiate in the presence of OTC in vitro.

ChemInform Abstract: The in vitro Trypanocidal Activity of Organotin Compounds.

ChemInform ◽  
2010 ◽  
Vol 28 (25) ◽  
pp. no-no
Author(s):  
J. SUSPERREGUI ◽  
A. PETSOM ◽  
M. BAYLE ◽  
G. LAIN ◽  
C. GIROUD ◽  
...  
Keyword(s):  
Organotin Compounds ◽  
Trypanocidal Activity

In vitro screening of organotin compounds and sediment extracts for cytotoxicity to fish cells

2010 ◽  
Vol 30 (1) ◽  
pp. 154-161 ◽  
Author(s):  
Michelle Giltrap ◽  
Ailbhe Macken ◽  
Brendan McHugh ◽  
Evin McGovern ◽  
Barry Foley ◽  
...  
Keyword(s):  
Organotin Compounds ◽  
In Vitro Screening ◽  
Fish Cells

An example of an integrated approach for health and environmental risk assessment: the case of organotin compounds

2000 ◽  
Vol 42 (7-8) ◽  
pp. 305-313
Author(s):  
J. Sekizawa ◽  
G. Suter ◽  
T. Vermeire ◽  
W. Munns
Keyword(s):  
Risk Assessment ◽  
Integrated Approach ◽  
Organotin Compounds ◽  
Health And Environmental Effects ◽  
Environmental Decision Making ◽  
Integrated Risk Assessment ◽  
Toxicological Data ◽  
Potential Risks ◽  
Available Information

Because environmental decision making based solely on simple compilation of toxicological data on either wildlife or humans in isolation can not give effective answers about the nature and levels of risk, an integrated approach for risk assessment of adverse effects of chemicals is required. Integration of available information on health and environmental effects, from in vitro to the level of humans, across various species, across different endpoints, and in combination with integrated exposure data, permits enhanced estimation of the potential risks posed by various agents. Mechanistic and quantitative consideration are the keys in this process. A framework for integrated risk assessment has been proposed by an international workgroup. The value and utility of the integrated approach is shown using the example of organotin compounds.

Direct and Indirect Assessment of the Aneuploidy-inducing Potency of Organotin Compounds

1991 ◽  
Vol 19 (2) ◽  
pp. 214-218
Author(s):  
Klaus Gjervig Jensen ◽  
Ole Andersen ◽  
Mogens Rønne
Keyword(s):  
Average Length ◽  
Human Lymphocytes ◽  
Organotin Compounds ◽  
Chromosome 1 ◽  
Tributyltin Chloride ◽  
Human Peripheral Lymphocytes ◽  
Triphenyltin Chloride ◽  
Spindle Function

The spindle-inhibiting and aneuploidy-inducing potency of in vitro exposure of PHA-stimulated human lymphocytes to organotin compounds has been studied indirectly by quantitation of chromosomal contraction and directly by determination of the frequency of aneuploidy by chromosome counting. The effects of trimethyltin chloride (TMT), dimethyltin chloride (DMT), tributyltin chloride (TBT), dibutyltin chloride (DBT), triphenyltin chloride (TPhT), and diphenyltin chloride (DPhT) on chromosal contraction were studied at concentrations of 10-3-10 9M, by measurement of the average length of chromosome 1 from asynchronous cultures of human peripheral lymphocytes. TMT, TBT, TPhT and DPhT appear to be very strong inducers of chromosomal supercontraction, indicating that these compounds are possible spindle inhibitors, while DMT and DBT seem to be ineffective. TMT, TBT, TPhT and DPhT gave rise to a little more than 100% increase in the number of hyperdiploid cells, all the increases being statistically significant except for that induced by TBT, indicating that organotin compounds are capable of inducing aneuploidy, probably by affecting spindle function.

In vitro cytotoxicity of organotin compounds to fish hepatoma cell une PLHC-1

1994 ◽  
Vol 74 ◽  
pp. 11
Author(s):  
B.J. Brüschweiler ◽  
F.E. Würgler ◽  
K. Fent
Keyword(s):  
Hepatoma Cell ◽  
In Vitro Cytotoxicity ◽  
Organotin Compounds

scholarly journals The effect of tributyltin on human eosinophylic leukemia EoL-1 cells

2008 ◽  
Vol 13 (1) ◽  
Author(s):  
Jolanta Sroka ◽  
Przemysław Włosiak ◽  
Anna Wilk ◽  
Justyna Antonik ◽  
Jarosław Czyż ◽  
...  
Keyword(s):  
Human Blood ◽  
Human Fibroblasts ◽  
Basic Function ◽  
Organotin Compounds ◽  
Cellular Model ◽  
Dose Dependent Decrease ◽  
Dose Dependent

AbstractOrganotin compounds are chemicals that are widely used in industry and agriculture as plastic stabilizers, catalysts and biocides. Many of them, including tributyltin (TBT), have been detected in human food and, as a consequence, detectable levels have been found in human blood. As organotin compounds were shown to possess immunotoxic activity, we focused our attention on the effect of TBT on the basic determinants of the function of eosinophils, i.e. cell adhesiveness and motility. We used human eosinophylic leukemia EoL-1 cells, a common in vitro cellular model of human eosinophils. Here, we demonstrate that TBT causes a dose-dependent decrease in the viability of EoL-1 cells. When administered at sub-lethal concentrations, TBT significantly decreases the adhesion of EoL-1 cells to human fibroblasts (HSFs) and inhibits their migration on fibroblast surfaces. Since the basic function of eosinophils is to invade inflamed tissues, our results indicate that TBT, and possibly other organotin compounds, may affect major cellular properties involved in the determination of in vivo eosinophil function.

Effects of organotin compounds on mitosis, spindle structure, toxicity and in vitro microtubule assembly

Mutagenesis ◽  
1991 ◽  
Vol 6 (5) ◽  
pp. 409-416 ◽  
Author(s):  
Klaus Gjervig Jensen ◽  
Agneta Önfelt ◽  
Margareta Wallin ◽  
Vitauts Lidumas ◽  
Ole Andersen
Keyword(s):  
Organotin Compounds ◽  
Microtubule Assembly ◽  
Spindle Structure
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