Membrane burdens of chlorinated benzenes lower the main phase transition temperature in dipalmitoyl-phosphatidylcholine vesicles: Implications for toxicity by narcotic chemicals

1996 ◽  
Vol 15 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Annemarie P. van Wezel ◽  
Gerard Cornelissen ◽  
J. Kees van Miltenburg ◽  
Antoon Opperhuizen
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Main Phase ◽  
Chlorinated Benzenes ◽  
Dipalmitoyl Phosphatidylcholine

scholarly journals The Effect of Selected Anions on Dipalmitoylphosphatidylcholine Phase Transitions

2002 ◽  
Vol 57 (7-8) ◽  
pp. 712-716 ◽  
Author(s):  
Adriana Przyczyna ◽  
Bożenna Różycka-Roszk ◽  
Marek Langner
Keyword(s):  
Phase Transition ◽  
Phase Transitions ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Salt Concentration ◽  
Half Width ◽  
Main Phase ◽  
Interface Properties ◽  
Transition Enthalpy ◽  
Peak Half Width

The effect of three anions, Cl-, Br- and I-, on the phase transitions of dipalmitoylphosphatidylcholine (DPPC) was measured. Main phase transition was modestly affected by these anions in the salt concentration range 0.2 M. For Cl- and Br- the temperature of main phase transition was lower (by about 0.5 °C), its half-width modestly larger and enthalpy practically unchanged, all three parameters were altered to a much larger degree. Main phase transition temperature was 1.5 °C lower and the peak half-width significantly smaller. These changes were not accompanied by any alteration in main phase transition enthalpy. Iodide shifted the pretransition temperature toward lower values and increased its half-width to such an extent that at concentrations above 100 mm it was practically undetectable. Besides cations, the presence of anions also has a distinct effect on lipid bilayer interface properties.

Do Low-Power Lasers Change Phase Transition Temperature of Dipalmitoyl Phosphatidylcholine (DPPC) Membrane?

1993 ◽  
Vol 11 (4) ◽  
pp. 191-195
Author(s):  
KAZUHIKO IIJIMA ◽  
NAOHITO SHIMOYAMA ◽  
MEGUMI SHIMOYAMA ◽  
TADANOBU MIZUGUCHI ◽  
KATSUHIRO TAMURA
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Low Power ◽  
Phase Transition Temperature ◽  
Dipalmitoyl Phosphatidylcholine

A Semisynthetic 5-n-Alkylresorcinol Derivative and its Effect upon Biomembrane Properties

2007 ◽  
Vol 62 (11-12) ◽  
pp. 881-888 ◽  
Author(s):  
Maria Stasiuk ◽  
Dominika Bartosiewicz ◽  
Jerzy Gubernator ◽  
Katarzyna Cieslik-Boczula ◽  
Martin Hof ◽  
...  
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Ph Value ◽  
Membrane Surface ◽  
Main Phase ◽  
Phospholipid Bilayer ◽  
Low Concentrations ◽  
Packing Defects ◽  
Semisynthetic Derivative

MSAR (1-sulfate-3-myristoyl-5-pentadecylbenzene) is a semisynthetic derivative of 5-npentadecylresorcinol (C15:0). MSAR exhibits hemolytic activity against sheep erythrocytes with a EH50 value of (35 ± 1.7) μm. At low concentrations MSAR also exhibits the ability to protect cells against their hypoosmotic lysis. This protective effect is significant as, at 0.1 μm of MSAR, the extent of osmotically induced cell lysis is reduced by approx. 20%. It was demonstrated that the 9-anthroyloxystearic acid signal was most intensively quenched by MSAR molecules, suggesting a relatively deep location of these molecules within the lipid bilayer. MSAR causes an increase of the fluorescence of the membrane potential sensitive probe. This indicates an alteration of the surface charge and a decrease of the local pH value at the membrane surface. At low bilayer content (1-4 mol%) this compound causes a significant increase of the phospholipid bilayer fluidity (both under and above the main phase transition temperature) of dipalmitoylphosphatidylcholine (DPPC) liposomes. At this low content MSAR slightly decreases the main phase transition temperature (Tc) value. The effects induced in the phospholipid bilayer by higher contents of MSAR molecules (5-10 mol%) make it impossible to determine the Tc value and to evaluate changes of the membrane fluidity by using pyrene-labeled lipid. MSAR also causes a decrease of the activity of membrane-bound enzymes-red blood cell acetylcholinesterase (AChE) and phospholipase A2 (PLA2). MSAR decreases the AChE activity by 40% at 100 μm. The presence of MSAR in the liposomal membrane induces a complete abolishment of the lag time of the PLA2 activity, indicating that these molecules induce the formation of packing defects in the bilayer which may result from imperfect mixing of phospholipids.

Effect of Sodium Cholate on the Phase Transition Temperature of Dipalmitoyl Phosphatidylcholine

1983 ◽  
Vol 38 (3-4) ◽  
pp. 302-306 ◽  
Author(s):  
Srabani Banerjee ◽  
S. N. Chatterjee
Keyword(s):  
Electron Microscopy ◽  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Sodium Cholate ◽  
Molar Ratio ◽  
Unilamellar Vesicles ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Multilamellar Liposomes

On treatment with sodium cholate, the phase transition temperature (Tc) of multilamellar liposomes derived from dipalmitoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P molar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles of DPPC exhibited the same Tc as that of the multilamellar ones. The formation of unilamellar vesicles from the multilam ellar ones by cholate treatm ent was investigated by electron microscopy

scholarly journals Determination of the Main Phase Transition Temperature of Phospholipids by Nanoplasmonic Sensing

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Wen Chen ◽  
Filip Duša ◽  
Joanna Witos ◽  
Suvi-Katriina Ruokonen ◽  
Susanne K. Wiedmer
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
Main Phase
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