scholarly journals Ingestion and Toxicity of Polystyrene Microplastics in Freshwater Bivalves

2021 ◽  
Author(s):  
Annkatrin Weber ◽  
Nina Jeckel ◽  
Carolin Weil ◽  
Simon Umbach ◽  
Nicole Brennholt ◽  
...  
Keyword(s):  
Freshwater Bivalves

Filtration rate capacities in 6 species of European freshwater bivalves

Oecologia ◽  
1988 ◽  
Vol 77 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Jakob Kryger ◽  
Hans Ulrik Riisg�rd
Keyword(s):  
Filtration Rate ◽  
Freshwater Bivalves

Identification of distinct haemocyte populations from the freshwater bivalves swan mussel (Anodonta cygnea) and duck mussel (Anodonta anatina) using wheat-germ agglutinin

2017 ◽  
Vol 95 (12) ◽  
pp. 937-947 ◽  
Author(s):  
M. Hinzmann ◽  
M. Lopes-Lima ◽  
F. Cerca ◽  
A. Correia ◽  
J. Machado ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Transmission Microscopy ◽  
Anodonta Cygnea ◽  
Functional Studies ◽  
Lectin Staining ◽  
Surface Staining ◽  
Cytological Features ◽  
Freshwater Bivalves

Haemocytes play a major role in molluscs immunity. Functional studies are, however, impaired by limited available experimental tools to identify and sort distinct haemocyte populations. Therefore, using nonlethal methods, we aimed at evaluating whether lectin staining combined with flow cytometry could be used to distinguish circulating haemocyte populations from two freshwater bivalves of the family Unionidae, the duck mussel (Anodonta anatina (L., 1758)) and the swan mussel (Anodonta cygnea (L., 1758)). Based on classical classification, haemocytes were distinguished as granulocytes and hyalinocytes and cytological features were visualized using transmission microscopy and staining techniques. Size, granularity, viability, and surface staining using lectins as specific probes were analysed by flow cytometry and fluorescence microscopy. The microscopic proportions of granulocytes and hyalinocytes significantly differed, being of 70% and 30% for A. cygnea and of 85% and 15% for A. anatina, respectively. Two haemocyte populations were sorted by flow cytometry based on size and granularity and confirmed as granulocytes and hyalinocytes. Interestingly, two different granulocyte populations could be further discriminated in A. cygnea according to their binding affinity to wheat-germ agglutinin (WGA), whereas granulocytes of A. anatina all stained similarly. Our results show that WGA labelling combined with flow cytometry can be used to better discriminate Anodonta haemocyte populations and obtain purified populations for functional studies.

The pitfall with PIT tags: marking freshwater bivalves for translocation induces short-term behavioural costs

2011 ◽  
Vol 81 (1) ◽  
pp. 341-346 ◽  
Author(s):  
Conor D. Wilson ◽  
Gareth Arnott ◽  
Neil Reid ◽  
Dai Roberts
Keyword(s):  
Short Term ◽  
Pit Tags ◽  
Freshwater Bivalves

scholarly journals Large-scale monitoring of freshwater bivalves: an eDNA point of view on species distribution and conservation

2020 ◽  
Author(s):  
Vincent Pri ◽  
Manuel Lopes Lima ◽  
Pierre Taberlet ◽  
Alice Valentini ◽  
Nicolas Poulet ◽  
...  
Keyword(s):  
Species Distribution ◽  
Large Scale ◽  
Point Of View ◽  
Freshwater Bivalves
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