Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination

2020 ◽  
Vol 39 (9) ◽  
pp. 1670-1677
Author(s):  
Pavlína Pelcová ◽  
Andrea Ridošková ◽  
Jana Hrachovinová ◽  
Jan Grmela
Keyword(s):  
Fractionation Analysis ◽  
Mercury Fraction ◽  
Bioavailable Mercury ◽  
Fraction Determination

Hairless is translocated to the nucleus via a novel bipartite nuclear localization signal and is associated with the nuclear matrix

2001 ◽  
Vol 114 (2) ◽  
pp. 367-376
Author(s):  
K. Djabali ◽  
V.M. Aita ◽  
A.M. Christiano
Keyword(s):  
Amino Acid ◽  
Hair Follicle ◽  
Nuclear Localization ◽  
Nuclear Matrix ◽  
Nuclear Localization Signal ◽  
Amino Acid Residues ◽  
Bipartite Nuclear Localization Signal ◽  
Hairless Gene ◽  
Fractionation Analysis ◽  
Localization Signal

Hair follicle cycling is an exquisitely regulated and dynamic process consisting of phases of growth, regression and quiescence. The transitions between the phases are governed by a growing number of regulatory proteins, including transcription factors. The hairless (hr) gene encodes a putative transcription factor that is highly expressed in the skin, where it appears to be an essential regulator during the regression of the catagen hair follicle. In hairless mice, as well as humans with congenital atrichia, the absence of hr gene function initiates a premature and abnormal catagen due to a dysregulation of apoptosis and cell adhesion, and defects in the signaling required for hair follicle remodeling. Here, we report structure-function studies of the hairless gene product, in which we identify a novel bipartite nuclear localization signal (NLS) of the form KRA(X13) PKR. Deletion analysis of the mouse hr gene mapped the NLS to amino acid residues 409–427. Indirect immunofluorescence microscopy of cells transiently transfected with hairless-green fluorescent fusion proteins demonstrated that these amino acid residues are necessary and sufficient for nuclear localization. Furthermore, nuclear fractionation analysis revealed that the hr protein is associated with components of the nuclear matrix.

Fouling Tendency of Ash Resulting From Burning Mixtures of Biofuels

2005 ◽  
Author(s):  
Mischa Theis ◽  
Bengt-Johan Skrifvars ◽  
Mikko Hupa ◽  
Honghi Tran
Keyword(s):  
Flow Reactor ◽  
Chemical Interaction ◽  
Substrate Surface ◽  
Agricultural Waste ◽  
Pulp Mill ◽  
Considerable Change ◽  
Chemical Fractionation ◽  
Feed Composition ◽  
Linear Pattern ◽  
Fractionation Analysis

Specified mixtures of peat with bark and peat with straw were burned in a lab-scale entrained flow reactor that simulates conditions in the superheater region of a biomass-fired boiler. Deposits were collected on an air-cooled probe that was inserted into the reactor at the outlet. For both mixtures, the deposition behaviour followed a non-linear pattern, which suggests that physico-chemical interaction between the ashes of the different fuels has taken place. The results indicate that it is possible to burn up to 30 wt-% bark (renewable biofuel and pulp mill waste) and up to 70 wt-% straw (renewable biofuel and agricultural waste) in mixtures with peat without encountering increased deposition rates in the reactor. The deposit composition was compared to the fuel ash composition using chemical fractionation analysis and SEM/EDX. While the composition of deposits obtained from pure fuels resembles the feed composition, a considerable change is observed in deposits obtained from mixtures. K and S compounds are attached to Si spheres and the substrate surface. The deposition rate is significantly lowered when removing K, S, Cl and Na in bark prior to burning by washing and mechanical/thermal dewatering.

scholarly journals InAsSb mole fraction determination using Raman low energy modes

2019 ◽  
Vol 10 (1) ◽  
pp. 149
Author(s):  
Kacper Grodecki ◽  
Krzysztof Murawski ◽  
Krystian Michalczewski ◽  
Bartłomiej Jankiewicz ◽  
Piotr Martyniuk
Keyword(s):  
Mole Fraction ◽  
Low Energy ◽  
Fraction Determination

Tracking transformation processes of organic micropollutants in aquatic environments using multi-element isotope fractionation analysis

2011 ◽  
Vol 26 ◽  
pp. S334-S336 ◽  
Author(s):  
Thomas B. Hofstetter ◽  
Jakov Bolotin ◽  
Marita Skarpeli-Liati ◽  
Reto Wijker ◽  
Zohre Kurt ◽  
...  
Keyword(s):  
Isotope Fractionation ◽  
Aquatic Environments ◽  
Organic Micropollutants ◽  
Fractionation Analysis ◽  
Transformation Processes

Abstract 311: Measurements of cAMP Dynamics in the SERCA2 Microdomain in Adult Mouse Cardiomyocytes using a Targeted FRET Biosensor

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Julia U Sprenger ◽  
Viacheslav O Nikolaev
Keyword(s):  
Transgenic Mice ◽  
Molecular Mechanisms ◽  
Ventricular Myocytes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Heart Weight ◽  
Cell Fractionation ◽  
Adrenergic Stimulation ◽  
Cardiac Phenotype ◽  
Fractionation Analysis

PURPOSE: cAMP is a central regulator of cardiac function and disease. This global second messenger acts in a compartmentalized fashion, and changes in cAMP dynamics are linked to cardiac diseases. In this project, we visualized cAMP signals directly in such microdomains to gain insights into the molecular mechanisms involved in cAMP compartmentation and its alterations in hypertrophy. Methods: We generated transgenic mice expressing a new Förster resonance energy transfer (FRET)-based cAMP sensor Epac1-camps-PLN to measure cAMP dynamics in the microdomain around the sarco/endoplasmic reticulum Ca2+-ATPase 2 (SERCA2). This sensor is targeted to SERCA2 via phospholamban (PLN). Results: Colocalization and cell fractionation analysis confirmed proper localization of the sensor in transgenic mouse hearts. qPCR analysis revealed a two-fold overexpression of PLN. However, no adverse cardiac phenotype could be detected by histological analysis and heart weight to body weight ratios. Local cAMP dynamics were measured using freshly isolated adult ventricular myocytes and compared to cAMP signals in the bulk cytosol using cardiomyocytes from Epac1-camps mice. We detected the predominant role of phosphodiesterases (PDEs) 4 and 3 in the SERCA2 compartment under basal conditions. These PDEs were responsible for shaping the microdomain and its segregation from the cytosolic compartment. Interestingly, beta1-adrenergic stimulation led to a stronger increase of local cAMP in the SERCA2 compartment compared to the bulk cytosol. 8 weeks after transverse aortic constriction (TAC), PDE4 activity was downregulated in the SERCA2 microdomain compared to sham cardiomyocytes. Conclusion: We successfully generated transgenic mice expressing the targeted Epac1-camps-PLN biosensor to visualize cAMP dynamics in the SERCA2 compartment. We could show distinct cAMP dynamics around the SERCA2 compartment compared to the bulk cytosol and uncovered its alterations in hypertrophied cardiomyocytes

scholarly journals Circulating Apolipoprotein L1 is associated with insulin resistance-induced abnormal lipid metabolism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kenji Nishimura ◽  
Taichi Murakami ◽  
Toshihiro Sakurai ◽  
Masashi Miyoshi ◽  
Kiyoe Kurahashi ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Metabolic Syndrome ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Insulin Resistant ◽  
Cell Dysfunction ◽  
Fractionation Analysis ◽  
Abnormal Lipid Metabolism ◽  
Hdl Metabolism ◽  
Relationship Of

Abstract Circulating ApolipoproteinL1 (ApoL1) is a component of pre-β-high-density lipoprotein (HDL), however little is known about the relationship of ApoL1 with cardiometabolic factors. Considering previous studies reporting the correlation of ApoL1 to triglyceride, we have hypothesized that ApoL1 associates with insulin-related metabolism. The current study examined their associations in 126 non-diabetic subjects and 36 patients with type 2 diabetes (T2DM). Non-diabetic subjects demonstrated triglyceride (standardized coefficients [s.c.] = 0.204, p < 0.05), body mass index (s.c. =0.232, p < 0.05) and HDL cholesterol (s.c. = −0.203, p < 0.05) as independent determinant of ApoL1 levels, and the significant elevation of ApoL1 in metabolic syndrome. Lipoprotein fractionation analysis revealed the predominant distribution of ApoL1 in large HDL fraction, and the significant increase of ApoL1 in large LDL fraction in high ApoL1 samples with insulin resistance. In T2DM, ApoL1 was higher in T2DM with metabolic syndrome, however ApoL1 was lower with β cell dysfunction. Insulin significantly promotes ApoL1 synthesis and secretion in HepG2 cells. In conclusion, circulating ApoL1 may be associated with abnormal HDL metabolism in insulin resistant status. This may suggest a regulation of insulin signal on the ApoL1 level, leading to offer a novel insight to the ApoL1 biology.

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