In ovo tris(2-butoxyethyl) phosphate concentrations significantly decrease in late incubation after a single exposure via injection, with no evidence of effects on hatching success or latent effects on growth or reproduction in zebra finches

2016 ◽  
Vol 36 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Margaret L. Eng ◽  
Robert J. Letcher ◽  
Tony D. Williams ◽  
John E. Elliott
Keyword(s):  
Hatching Success ◽  
Zebra Finches ◽  
Single Exposure ◽  
In Ovo ◽  
Latent Effects

In ovo exposure to brominated flame retardants Part I: Assessment of effects of TBBPA-BDBPE on survival, morphometric and physiological endpoints in zebra finches

2019 ◽  
Vol 179 ◽  
pp. 104-110 ◽  
Author(s):  
Margaret L. Eng ◽  
Tony D. Williams ◽  
Kim J. Fernie ◽  
Natalie K. Karouna Renier ◽  
Paula F.P. Henry ◽  
...  
Keyword(s):  
Flame Retardants ◽  
Brominated Flame Retardants ◽  
Zebra Finches ◽  
In Ovo

Assessment of neuroanatomical and behavioural effects of in ovo methylmercury exposure in zebra finches ( Taeniopygia guttata )

2017 ◽  
Vol 59 ◽  
pp. 33-39 ◽  
Author(s):  
Maria S. Yu ◽  
Margaret L. Eng ◽  
Tony D. Williams ◽  
Mélanie F. Guigueno ◽  
John E. Elliott
Keyword(s):  
Taeniopygia Guttata ◽  
Zebra Finches ◽  
In Ovo ◽  
Behavioural Effects ◽  
Methylmercury Exposure

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Three-dimensional reconstruction of the 40S ribosomal subunit from rabbit reticulocytes

1987 ◽  
Vol 45 ◽  
pp. 936-937
Author(s):  
Neng-Yu Zhang ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
Tom Obrig ◽  
Joachim Frank
Keyword(s):  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Uranyl Acetate ◽  
Reconstruction Method ◽  
Single Exposure ◽  
Electron Dose ◽  
Ribosomal Subunits ◽  
40S Ribosomal Subunit ◽  
Dimensional Reconstruction ◽  
Rabbit Reticulocytes

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.

Early life nutritional stress affects song learning but not underlying neural circuitry in zebra finches.

2020 ◽  
Vol 134 (3) ◽  
pp. 222-232
Author(s):  
Khulganaa Buyannemekh ◽  
Jessica B. Zito ◽  
Michelle L. Tomaszycki
Keyword(s):  
Early Life ◽  
Nutritional Stress ◽  
Neural Circuitry ◽  
Song Learning ◽  
Zebra Finches
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