Introduction of moribund category to OECD fish acute test and its effect on suffering and LC50 values

2012 ◽  
Vol 31 (5) ◽  
pp. 1107-1112 ◽  
Author(s):  
Hans Rufli
Keyword(s):  
Acute Test

scholarly journals Comparison of Joint Effect of Acute and Chronic Toxicity for Combined Assessment of Heavy Metals on Photobacterium sp.NAA-MIE

2021 ◽  
Vol 18 (12) ◽  
pp. 6644
Author(s):  
Nur Adila Adnan ◽  
Mohd Izuan Effendi Halmi ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Yunus Abd Shukor
Keyword(s):  
Heavy Metals ◽  
Chronic Toxicity ◽  
Mixture Toxicity ◽  
Joint Effect ◽  
Antagonistic Effect ◽  
Acute Test ◽  
Acute And Chronic Toxicity ◽  
Toxicological Studies ◽  
Luminescence Inhibition ◽  
Toxicity Unit

Predicting the crucial effect of single metal pollutants against the aquatic ecosystem has been highly debatable for decades. However, dealing with complex metal mixtures management in toxicological studies creates a challenge, as heavy metals may evoke greater toxicity on interactions with other constituents rather than individually low acting concentrations. Moreover, the toxicity mechanisms are different between short term and long term exposure of the metal toxicant. In this study, acute and chronic toxicity based on luminescence inhibition assay using newly isolated Photobacterium sp.NAA-MIE as the indicator are presented. Photobacterium sp.NAA-MIE was exposed to the mixture at a predetermined ratio of 1:1. TU (Toxicity Unit) and MTI (Mixture Toxic Index) approach presented the mixture toxicity of Hg2+ + Ag+, Hg2+ + Cu2+, Ag+ + Cu2+, Hg2+ + Ag+ + Cu2+, and Cd2+ + Cu2+ showed antagonistic effect over acute and chronic test. Binary mixture of Cu2+ + Zn2+ was observed to show additive effect at acute test and antagonistic effect at chronic test while mixture of Ni2+ + Zn2+ showing antagonistic effect during acute test and synergistic effect during chronic test. Thus, the strain is suitable and their use as bioassay to predict the risk assessment of heavy metal under acute toxicity without abandoning the advantage of chronic toxicity extrapolation.

scholarly journals Somatostatin receptors subtypes 2 and 5, dopamine receptor type 2 expression and gsp status as predictors of octreotide LAR® responsiveness in acromegaly

2008 ◽  
Vol 52 (8) ◽  
pp. 1288-1295 ◽  
Author(s):  
Leonardo Vieira Neto ◽  
Giselle Fernandes Taboada ◽  
Mônica Roberto Gadelha
Keyword(s):  
Growth Hormone ◽  
Quantitative Analysis ◽  
Dopamine Receptor ◽  
Molecular Analysis ◽  
Somatostatin Receptor ◽  
Somatostatin Receptors ◽  
Laboratory Data ◽  
Octreotide Lar ◽  
Acute Test ◽  
Igf I

We present two acromegalic patients in which clinical and molecular data are discussed in regard to their ability to predict long term octreotide LAR® therapy response. Case reports: Patient 1: female, 36 years old at diagnosis. Basal GH and IGF-I at diagnosis were 133 ng/mL and 181% above the upper limit of reference values (ULRV), respectively. Growth hormone during acute test with subcutaneous octreotide decreased from 133 to 13 ng/mL. Patient started on primary octreotide LAR® therapy (20mg q28 days) and achieved biochemical parameters of disease control after 6 months. Molecular analysis of tumor fragments: gsp +; quantitative analysis of SSTR (somatostatin receptor) and DR (dopamine receptor) mRNA - SSTR2 23954; SSTR5 2407; DR2 total 17016 copies. Patient 2: male, 38 years old at diagnosis. Basal GH and IGF-I at diagnosis were 120 ng/mL and 114% ULRV, respectively. Patient underwent non-curative trans-sphenoidal surgery. Post-operative GH and IGF-I were 112 ng/mL and 137% ULRV, respectively. Growth hormone during acute test with subcutaneous octreotide decreased from 112 to 7 ng/mL. Octreotide LAR® therapy (20 mg q28 days) was then initiated. After 6 months of treatment, patient did not attain biochemical control of disease and displayed increased tumor volume. Molecular analysis of tumor fragments: gsp not done; quantitative analysis of SSTR and DR mRNA - SSTR2 416; SSTR5 3767; DR2 total 3439 copies. In conclusion, these two cases illustrate how laboratory data can be conflicting as predictors of octreotide LAR® responsiveness and how molecular analysis of tumor fragments can help explain different behaviors in clinically similar patients.

The effect of acid stress on survivorship and reproduction of Daphnia pulex (Crustacea: Cladocera)

1982 ◽  
Vol 60 (4) ◽  
pp. 573-579 ◽  
Author(s):  
W. E. Walton ◽  
S. M. Compton ◽  
J. D. Allan ◽  
R. E. Daniels
Keyword(s):  
Population Growth Rate ◽  
Daphnia Pulex ◽  
Acid Stress ◽  
Pond Water ◽  
Low Ph ◽  
Laboratory Simulation ◽  
Zooplankton Species ◽  
Field Surveys ◽  
Delayed Onset ◽  
Acute Test

Laboratory simulation of acid stress to a common cladoceran, Daphnia pulex, was conducted in pond water of varying acidity due to addition of H2SO4. An acute test using exposure times of 1 to 96 h and pH levels of 3.7 and 6.5 revealed virtually no effect at 4.3 and higher, while 4.2 and lower severely reduced survivorship. Very short (3-h) exposures caused nearly complete mortality at pH 3.7, while > 12 h exposure caused high mortality at pH of 4.0–4.2. A chronic 21-d life table test indicated a gradually increasing impairment of population growth rate potential (r) at pH 5.0 and below. This was due primarily to reduced survivorship and delayed onset of reproductive maturity. Those individuals which survived at lower pH levels produced broods equivalent to unstressed daphnids. While the tolerance of D. pulex to low pH does not appear to have been reported from field surveys, our results are in close agreement with the observed effects of increasing acidity on the distribution of other zooplankton species.

Comparative sensitivities of larval stages of the cane toad, Rhinella marina, and the striped marsh frog, Limnodynastes peronii, to atrazine

2013 ◽  
Vol 61 (4) ◽  
pp. 320 ◽  
Author(s):  
Khurshida Akter Siddiqua ◽  
Ralph Alquezar ◽  
Scott Paton Wilson
Keyword(s):  
Native Species ◽  
Test Design ◽  
Cane Toad ◽  
Test Species ◽  
Rhinella Marina ◽  
Marsh Frog ◽  
Larval Stages ◽  
Acute Test ◽  
Metamorphic Climax ◽  
Solvent Control

Variations in larval sensitivities to atrazine were determined in the Australian native striped marsh frog, Limnodynastes peronii, and the introduced cane toad, Rhinella marina. The static acute test design involved six nominal concentrations of atrazine, including control, solvent control, 3, 6, 12, and 24 mg L–1. Gosner stages 22–23 as hatchlings, stages 25–26, 28–29, and 32–33 as premetamorphic, 36–37 as prometamorphic and 40–41 as metamorphic climax stages of cane toads and the first four sets of Gosner stages of striped marsh frogs were exposed to atrazine treatments for 96 h. Results showed that late larval stages were more sensitive than early stages and different premetamorphic stages showed variations in sensitivities in both test species. The striped marsh frog showed a stronger concentration- and stage-dependent response and greater sensitivity to atrazine than the cane toad. In both experimental species, Gosner stages 28–29 showed better concentration-dependent increase in sensitivities to atrazine compared with other larval stages. It can be concluded that inter- and intra-species variations in sensitivities to atrazine may occur in Australian anurans and native species may show greater sensitivity to acute concentrations of atrazine than the introduced cane toad.

scholarly journals The year public health lost its soul

2021 ◽  
Author(s):  
Damien Contandriopoulos
Keyword(s):  
Public Health ◽  
Acute Test ◽  
Pandemic Response

The COVID pandemic was an acute test of public health capacities worldwide. Many will hail the successes obtained and stress the importance of the discipline. On the contrary, this commentary defends the idea that the COVID pandemic response forced public health to enter in a Faustian bargain with governments and realpolitik that threaten the very core of the discipline’s principles.

scholarly journals Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

2021 ◽  
Vol 11 (3) ◽  
pp. 31-40
Author(s):  
Haqeeq Ahmad ◽  
Abdul Wadud ◽  
Nasreen Jahan ◽  
Ghulamuddin Sofi ◽  
, Hamiduddin
Keyword(s):  
Acute Toxicity ◽  
Classical Method ◽  
Test Group ◽  
Toxicity Study ◽  
Muffle Furnace ◽  
Acute Test ◽  
Acute Toxicity Study ◽  
Significant Difference ◽  
Starting Dose ◽  
Group B

  Background: Though a number of studies has been carried out in terms of safety of Kushta but concerns have been raised about the presence of heavy metals in Unani formulations. Classical method of preparation of KKH and other Kushta preparation in Unani system of medicine has been found to eliminate all such issues from the end product. Hence; the present study was designed to evaluate the acute and sub-acute toxicity study of KKH prepared by Classical and Muffle furnace methods. Objective: To ascertain the safety profile of Kushta Khabasul Hadeed (KKH) prepared by classical and muffle furnace methods. Materials and methods: Both of the samples of KKH i.e. Kushta Khabasul Hadeed (KKHCM) and Kushta Khabasul Hadeed (KKHMFM) prepared by Classical and Muffle Furnace Methods respectively were evaluated for acute and sub-acute toxicity by OECD guidelines 423 and 407 respectively. The starting dose in the acute test was 50 mg/kg and in sub-acute test, it was 134mg, 200 mg and 400 mg/kg b.w. Effect of both of the test samples were assessed for ponderal changes, food and water intake, relative organs weights, hematological, biochemical and histopathological features of various organs. Results: Both of the samples did not produce any sign of toxicity in acute toxicity study up to the dose level of 2000mg/kg b.w. In sub-acute toxicity study no mortality in any of the test groups was observed. KKHCM at different doses showed a slight decrease in food intake in all the test groups but significant difference (P<0.05) was observed in the test group B as compared to plain control. Conclusion: KKHMFM was found to be relatively toxic than KKHCM at different dose levels. The study demonstrated that both the Classical and Muffle furnace methods of preparation of Kushta are useful for preparing KKH and also safe at the dose prescribed in Unani literature. However, the Kushta prepared by the classical method was found to be safer than the muffle furnace method.

Effects of Ankle Taping on Single and Double Leg Balance

2010 ◽  
Vol 19 (1-2) ◽  
pp. 5-19
Author(s):  
Adam Knight ◽  
Wendi Weimar
Keyword(s):  
Center Of Gravity ◽  
Ankle Sprains ◽  
Acute Test ◽  
Eyes Closed ◽  
Athletic Tape ◽  
Different Types ◽  
Post Test ◽  
Eyes Open ◽  
Support Conditions ◽  
Healthy Participants

Effects of Ankle Taping on Single and Double Leg Balance Ankle sprains are a common injury and athletic tape is often applied to help prevent this injury, however, the effects of ankle taping on balance are relatively unclear. Additionally, the dominant and non-dominant legs often have different demands placed upon them and may yield different balance scores. Twenty five healthy participants (18 female, 7 male; age=20.5±1.19 years; mass=69.24±12.72 kg; height=1.69±.087 m) completed double and single leg balance assessments with the eyes open and eyes closed under three ankle support conditions: no ankle tape, PowerTape™, and Coach™ Tape, on the Neurocom Basic BalanceMaster™. Balance assessments were completed before ankle taping (pre-test), immediately after ankle taping (acute-test), and after 20 minutes of walking (post-test). The sway velocity of the participants' center of gravity (deg/s) was the dependent variable. A significant three way interaction was found for the single leg, eyes closed assessment (P = .037), with increased sway velocity for the PowerTape™ and Coach™ tape condition. The non-dominant leg had significantly less sway velocity than the dominant leg for the eyes open condition (P < .001). These results indicate differing affects on balance for different types of athletic tape, and balance differences between the dominant and non-dominant leg, with the non-dominant leg presenting lower sway velocities.

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