scholarly journals Ethanol production from sugarcane bagasse by fed-batch simultaneous saccharification and fermentation at high solids loading

2018 ◽  
Vol 6 (6) ◽  
pp. 810-818 ◽  
Author(s):  
Yueshu Gao ◽  
Jingliang Xu ◽  
Zhenhong Yuan ◽  
Jianchun Jiang ◽  
Zhenjia Zhang ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Simultaneous Saccharification And Fermentation ◽  
High Solids ◽  
Fed Batch ◽  
Solids Loading ◽  
High Solids Loading ◽  
Simultaneous Saccharification

scholarly journals Ethanol Production from High Solids Loading of Alkali-Pretreated Sugarcane Bagasse with an SSF Process

BioResources ◽  
2014 ◽  
Vol 9 (2) ◽  
Author(s):  
Yueshu Gao ◽  
Jingliang Xu ◽  
Zhenhong Yuan ◽  
Yu Zhang ◽  
Cuiyi Liang ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading ◽  
Pretreated Sugarcane Bagasse ◽  
Ssf Process

scholarly journals Enhanced sugars production with high conversion efficiency from alkali-pretreated sugarcane bagasse by enzymatic mixtures

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 3839-3849 ◽  
Author(s):  
Yunyun Liu ◽  
Yunqi Cao ◽  
Qiang Yu ◽  
Jingliang Xu ◽  
Zhenhong Yuan
Keyword(s):  
Conversion Efficiency ◽  
Sugarcane Bagasse ◽  
High Solids ◽  
Fed Batch ◽  
Total Sugars ◽  
Solids Loading ◽  
High Solids Loading ◽  
Substrate Conversion ◽  
Pretreated Sugarcane Bagasse ◽  
Conversion Efficiencies

Complementary enzymes can considerably enhance the hydrolysis effectiveness of cellulase. The influence of hemicellulase supplementation on high solids saccharification of alkali-pretreated sugarcane bagasse was assessed. Hemicellulase addition of 1200 IU/g substrate with cellulase loading of 10 FPU/g substrate achieved high sugars yield with glucose and xylose conversion efficiency of 95.4% and 87.4%, respectively. To further improve the substrate conversion efficiency based on high sugars production, fed-batch hydrolysis was employed with high solids loading of 20% (w/v) to 25% (w/v). After 96 h hydrolysis with 25% solids loading at cellulase and hemicellulase loading of 20 FPU/g and 1200 IU/g substrate, respectively, the obtained highest total sugars was 242 g/L, with glucose and xylose conversion efficiencies of 98.6% and 94.9%, respectively. An increase in substrate digestibility upon supplementation of mixture enzymes with high sugars production can be realized in high solids fed-batch system with proper cellulase and hemicellulase synergism.

scholarly journals Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol

2017 ◽  
Vol 114 (44) ◽  
pp. 11673-11678 ◽  
Author(s):  
Thanh Yen Nguyen ◽  
Charles M. Cai ◽  
Rajeev Kumar ◽  
Charles E. Wyman
Keyword(s):  
Ethanol Production ◽  
Product Inhibition ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading ◽  
Separate Hydrolysis And Fermentation ◽  
Ethanol Yields ◽  
Solid Biomass ◽  
Tolerance To Ethanol ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation (SSF) of solid biomass can reduce the complexity and improve the economics of lignocellulosic ethanol production by consolidating process steps and reducing end-product inhibition of enzymes compared with separate hydrolysis and fermentation (SHF). However, a long-standing limitation of SSF has been too low ethanol yields at the high-solids loading of biomass needed during fermentation to realize sufficiently high ethanol titers favorable for more economical ethanol recovery. Here, we illustrate how competing factors that limit ethanol yields during high-solids fermentations are overcome by integrating newly developed cosolvent-enhanced lignocellulosic fractionation (CELF) pretreatment with SSF. First, fed-batch glucose fermentations by Saccharomyces cerevisiae D5A revealed that this strain, which has been favored for SSF, can produce ethanol at titers of up to 86 g⋅L−1. Then, optimizing SSF of CELF-pretreated corn stover achieved unprecedented ethanol titers of 79.2, 81.3, and 85.6 g⋅L−1 in batch shake flask, corresponding to ethanol yields of 90.5%, 86.1%, and 80.8% at solids loadings of 20.0 wt %, 21.5 wt %, and 23.0 wt %, respectively. Ethanol yields remained at over 90% despite reducing enzyme loading to only 10 mg protein⋅g glucan−1 [∼6.5 filter paper units (FPU)], revealing that the enduring factors limiting further ethanol production were reduced cell viability and glucose uptake by D5A and not loss of enzyme activity or mixing issues, thereby demonstrating an SSF-based process that was limited by a strain’s metabolic capabilities and tolerance to ethanol.

Simultaneous Saccharification and Fermentation of Pretreated Eucalyptus grandis Under High Solids Loading

2017 ◽  
Vol 13 (3) ◽  
pp. 131-140 ◽  
Author(s):  
Shane McIntosh ◽  
Janice Palmer ◽  
Zhanying Zhang ◽  
William O.S. Doherty ◽  
Syed S. Yazdani ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Eucalyptus Grandis ◽  
High Solids ◽  
Solids Loading ◽  
High Solids Loading ◽  
Simultaneous Saccharification
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