Methods for the determination of metals and inorganic chemicals in environmental samples. Environmental Monitoring Systems Laboratory, Environmental Protection Agency, Noyes Publications, Park Ridge, NJ, (1996), 535 Pages [ISBN No.: 0 8155-1398-4], U.S. List Price: $64.00

1998 ◽  
Vol 17 (4) ◽  
pp. W9-W10
Author(s):  
Laura R. Skubal
Keyword(s):  
Environmental Monitoring ◽  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Environmental Samples ◽  
List Price ◽  
Monitoring Systems ◽  
Protection Agency ◽  
Park Ridge

The Use of Aniline Blue for the Determination of Dead Phytoplankton, Zooplankton and Meroplankton in LC50 Testings After 96 Hours… A Re-Evaluation of the US Environmental Protection Agency Methodology

2004 ◽  
Vol 126 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Balram Seepersad ◽  
Kelvin Ramnath ◽  
Shyam Dyal ◽  
Reeza Mohammed
Keyword(s):  
Environmental Protection ◽  
Environmental Protection Agency ◽  
Staining Technique ◽  
Aniline Blue ◽  
Protection Agency ◽  
Lc50 Value ◽  
The Us ◽  
Visual Observations

There is a need for a reliable staining technique to distinguish between live and dead organisms following LC50 tests. This is especially so in cases where organisms can be stressed or even become unconscious and appear dead to the aided or naked eyes. Visual observations under such conditions can result in an LC50 value shifting to the lower concentration thereby imposing stiffer guidelines for compliance. Aniline blue can only stain individuals which are physiologically dead imposing an accurate live-dead evaluation and producing a true LC50 value. Guidelines imposed using such data will facilitate compliance and provide an accurate value for an LC50.

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