scholarly journals Influence of ore grade and mineral medium on chalcopyrite bioleaching with mixed microbial consortia

2021 ◽  
Author(s):  
Eva Benzal ◽  
Montse Solé ◽  
Conxita Lao ◽  
Eloi Morral ◽  
Xavier Gamisans ◽  
...  
Keyword(s):  
Mineral Medium ◽  
Microbial Consortia ◽  
Ore Grade

scholarly journals Sustained dechlorination of vinyl chloride to ethene inDehalococcoides-enriched cultures grown without addition of exogenous vitamins and at low pH

2019 ◽  
Author(s):  
Luz A. Puentes Jácome ◽  
Po-Hsiang Wang ◽  
Olivia Molenda ◽  
Yi Xuan (Jine-Jine) Li ◽  
M. Ahsanul Islam ◽  
...  
Keyword(s):  
Vinyl Chloride ◽  
Low Ph ◽  
Mineral Medium ◽  
Ex Situ ◽  
Microbial Consortia ◽  
Neutral Ph ◽  
Cell Extracts ◽  
Enriched Cultures ◽  
Lower Ligand ◽  
Groundwater Pollutant

ABSTRACTTrichloroethene (TCE) is a ubiquitous groundwater pollutant. Successful TCE bioremediation has been demonstrated at field sites using specialized microbial consortia harboring TCE-respiringDehaloccocoideswhose growth is cobalamin (vitamin B12)-dependent. Bioaugmentation cultures grown ex situ with ample exogenous vitamins in the medium and at neutral pH may become vitamin-limited or inhibited by acidic pH once injected into field sites, resulting in incomplete TCE dechlorination and accumulation of more toxic vinyl chloride (VC). Here, we report growth of theDehalococcoides-containing bioaugmentation culture KB-1 in a TCE-amended mineral medium devoid of vitamins and in a VC-amended mineral medium at low pH (6.0 and 5.5). In cultures grown without exogenous vitamins or cobalamin,Acetobacterium, which can synthesize 5,6-dimethylbenzimidazole (DMB), the lower ligand of cobalamin, andSporomusaare the dominant acetogens. At neutral pH, a growingAcetobacteriumpopulation supports complete TCE dechlorination byDehalococcoidesat millimolar levels with a substantial increase in the amount of measured cobalamin (~20-fold). Sustained dechlorination of VC to ethene was achieved at a pH as low as 5.5, yet at low pHAcetobacteriumis less abundant, potentially affecting the production of DMB and/or cobalamin. However, dechlorination activity at very low pH (< 5.0) was not stimulated by DMB supplementation, but was restored by raising pH to neutral. Assays in cell extracts revealed that vinyl chloride reductase (VcrA) activity declines significantly below pH 6.0 and is undetectable below pH 5.0. This study highlights the roles of and interplay between vitamin-producing populations and pH in microbial dechlorinating communities, and their importance for successful chlorinated ethenes bioremediation at field sites.

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

Learning From Nature: Using Microbial Consortia To Improve Biochemical Synthesis

2018 ◽  
Author(s):  
Nicholas A. Kaplan ◽  
Alexandra M. Adams ◽  
J. Andrew Jones
Keyword(s):  
Microbial Consortia

An efficient biotreatment process for polyvinyl alcohol containing textile wastewater

2013 ◽  
Vol 8 (3-4) ◽  
pp. 469-478 ◽  
Author(s):  
Sandip S. Magdum ◽  
Gauri P. Minde ◽  
Upendra S. Adhyapak ◽  
V. Kalyanraman
Keyword(s):  
Polyvinyl Alcohol ◽  
Process Optimization ◽  
Textile Wastewater ◽  
Cost Effective ◽  
Chemical Oxidation ◽  
High Rate ◽  
Microbial Consortia ◽  
Environment Friendly ◽  
Candida Sp ◽  
Textile Wastewater Treatment

The aim of this work was to optimize the biodegradation of polyvinyl alcohol (PVA) containing actual textile wastewater for a sustainable treatment solution. The isolated microbial consortia of effective PVA degrader namely Candida Sp. and Pseudomonas Sp., which were responsible for symbiotic degradation of chemical oxidation demand (COD) and PVA from desizing wastewater. In the process optimization, the maximum aeration was essential to achieve a high degradation rate, where as stirring enhances further degradation and foam control. Batch experiments concluded with the need of 16 lpm/l and 150 rpm of air and stirring speed respectively for high rate of COD and PVA degradation. Optimized process leads to 2 days of hydraulic retention time (HRT) with 85–90% PVA degradation. Continuous study also confirmed above treatment process optimization with 85.02% of COD and 90.3% of PVA degradation of effluent with 2 days HRT. This study gives environment friendly and cost effective solution for PVA containing textile wastewater treatment.

scholarly journals Acinetobacter tandoii ZM06 Assists Glutamicibacter nicotianae ZM05 in Resisting Cadmium Pressure to Preserve Dipropyl Phthalate Biodegradation

2021 ◽  
Vol 9 (7) ◽  
pp. 1417
Author(s):  
Xuejun Wang ◽  
Si Shen ◽  
Hao Wu ◽  
Haixia Wang ◽  
Lvjing Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heavy Metals ◽  
Microbial Consortia ◽  
Environmental Media ◽  
Degrading Bacterium ◽  
Coculture System ◽  
Heavy Metals Contamination ◽  
Metabolite Inhibition ◽  
Comparative Transcriptomic Analysis ◽  
Scanning Electron

Dipropyl phthalate (DPrP) coexists with cadmium as cocontaminants in environmental media. A coculture system including the DPrP-degrading bacterium Glutamicibacter nicotianae ZM05 and the nondegrading bacterium Acinetobacter tandoii ZM06 was artificially established to degrade DPrP under Cd(II) stress. Strain ZM06 relieved the pressure of cadmium on strain ZM05 and accelerated DPrP degradation in the following three ways: first, strain ZM06 adsorbed Cd(II) on the cell surface (as observed by scanning electron microscopy) to decrease the concentration of Cd(II) in the coculture system; second, the downstream metabolites of ZM05 were utilized by strain ZM06 to reduce metabolite inhibition; and third, strain ZM06 supplied amino acids and fatty acids to strain ZM05 to relieve stress during DPrP degradation, which was demonstrated by comparative transcriptomic analysis. This study provides an elementary understanding of how microbial consortia improve the degradation efficiency of organic pollutants under heavy metals contamination.

scholarly journals Metagenome-assembled genomes infer potential microbial metabolism in alkaline sulphidic tailings

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Wenjun Li ◽  
Xiaofang Li
Keyword(s):  
Community Structure ◽  
Mine Tailings ◽  
Fluorescent In Situ Hybridization ◽  
High Throughput Sequencing ◽  
Microbial Consortia ◽  
Metabolic Reconstruction ◽  
Metal Release ◽  
Community Members ◽  
Oxidative Stresses

Abstract Background Mine tailings are hostile environment. It has been well documented that several microbes can inhabit such environment, and metagenomic reconstruction has successfully pinpointed their activities and community structure in acidic tailings environments. We still know little about the microbial metabolic capacities of alkaline sulphidic environment where microbial processes are critically important for the revegetation. Microbial communities therein may not only provide soil functions, but also ameliorate the environment stresses for plants’ survival. Results In this study, we detected a considerable amount of viable bacterial and archaeal cells using fluorescent in situ hybridization in alkaline sulphidic tailings from Mt Isa, Queensland. By taking advantage of high-throughput sequencing and up-to-date metagenomic binning technology, we reconstructed the microbial community structure and potential coupled iron and nitrogen metabolism pathways in the tailings. Assembly of 10 metagenome-assembled genomes (MAGs), with 5 nearly complete, was achieved. From this, detailed insights into the community metabolic capabilities was derived. Dominant microbial species were seen to possess powerful resistance systems for osmotic, metal and oxidative stresses. Additionally, these community members had metabolic capabilities for sulphide oxidation, for causing increased salinity and metal release, and for leading to N depletion. Conclusions Here our results show that a considerable amount of microbial cells inhabit the mine tailings, who possess a variety of genes for stress response. Metabolic reconstruction infers that the microbial consortia may actively accelerate the sulphide weathering and N depletion therein.

scholarly journals Synthetic neural-like computing in microbial consortia for pattern recognition

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ximing Li ◽  
Luna Rizik ◽  
Valeriia Kravchik ◽  
Maria Khoury ◽  
Netanel Korin ◽  
...  
Keyword(s):  
Neural Networks ◽  
Artificial Neural Networks ◽  
Gradient Descent ◽  
Biological Systems ◽  
Logic Gates ◽  
Structural Similarity ◽  
Microbial Consortia ◽  
Promoter Strength ◽  
Chemical Inducers ◽  
Artificial Neural

AbstractComplex biological systems in nature comprise cells that act collectively to solve sophisticated tasks. Synthetic biological systems, in contrast, are designed for specific tasks, following computational principles including logic gates and analog design. Yet such approaches cannot be easily adapted for multiple tasks in biological contexts. Alternatively, artificial neural networks, comprised of flexible interactions for computation, support adaptive designs and are adopted for diverse applications. Here, motivated by the structural similarity between artificial neural networks and cellular networks, we implement neural-like computing in bacteria consortia for recognizing patterns. Specifically, receiver bacteria collectively interact with sender bacteria for decision-making through quorum sensing. Input patterns formed by chemical inducers activate senders to produce signaling molecules at varying levels. These levels, which act as weights, are programmed by tuning the sender promoter strength Furthermore, a gradient descent based algorithm that enables weights optimization was developed. Weights were experimentally examined for recognizing 3 × 3-bit pattern.

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