Effect of combined inorganic with organic acids pretreatment of rice straw on its structure properties and enzymatic hydrolysis

2017 ◽  
Vol 37 (2) ◽  
pp. 808-814 ◽  
Author(s):  
Yun Li ◽  
Benkun Qi ◽  
Jie Feng ◽  
Yi Zhang ◽  
Yinhua Wan
2016 ◽  
Vol 87 ◽  
pp. 247-254 ◽  
Author(s):  
Plaimein Amnuaycheewa ◽  
Rotchanaphan Hengaroonprasan ◽  
Kittipong Rattanaporn ◽  
Suchata Kirdponpattara ◽  
Kraipat Cheenkachorn ◽  
...  

RSC Advances ◽  
2017 ◽  
Vol 7 (80) ◽  
pp. 50537-50545 ◽  
Author(s):  
Forough Momayez ◽  
Keikhosro Karimi ◽  
Shiva Karimi ◽  
Ilona Sárvári Horváth

Effects of biogas effluent and its organic ingredients, i.e., acetic, butyric, lactic, and propionic acid, for the pretreatment of rice straw on enzymatic hydrolysis and ethanol production was studied.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.


2003 ◽  
Vol 105 (1-3) ◽  
pp. 155-164 ◽  
Author(s):  
Betzabé Sulbarán-De-Ferrer ◽  
Marielena Aristiguieta ◽  
Bruce E. Dale ◽  
Alexis Ferrer ◽  
Graciela Ojeda-De-Rodriguez

Author(s):  
Shohreh Ariaeenejad ◽  
Atefeh Sheykhabdolahzadeh ◽  
Morteza Maleki ◽  
Kaveh Kavousi ◽  
Mehdi Foroozandeh Shahraki ◽  
...  

Abstract Background: Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results: The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. Using a multi-step in-silico analysis, primary candidates with desired properties were found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes' kinetic parameters, including V max , Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85°C and was able to retain more than 70% of its activity after 150 hours of storage at 85°C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl, MgCl 2 , CaCl 2 , and MnCl 2 . Our results showed that treatment with MnCl 2 could enhance the enzyme’s activity by 89%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 hours in the harsh condition ( T= 85°C, pH = 8.5). Conclusion: Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.


2012 ◽  
Vol 123 ◽  
pp. 636-645 ◽  
Author(s):  
Kyoung-Mi Lee ◽  
Dayanand Kalyani ◽  
Manish Kumar Tiwari ◽  
Tae-Su Kim ◽  
Saurabh Sudha Dhiman ◽  
...  

2018 ◽  
Vol 265 ◽  
pp. 394-398 ◽  
Author(s):  
Yu-Yan Gan ◽  
Si-Li Zhou ◽  
Xiao Dai ◽  
Han Wu ◽  
Zi-Yao Xiong ◽  
...  

Author(s):  
Jaruwan Damaurai ◽  
Thanchanok Preechakun ◽  
Marisa Raita ◽  
Verawat Champreda ◽  
Navadol Laosiripojana

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