Interlaboratory studies with the chinese hamster v79 cell metabolic cooperation assay to detect tumor-promoting agents

1988 ◽  
Vol 12 (1) ◽  
pp. 33-51 ◽  
Author(s):  
J. S. Bohrman ◽  
J. R. Burg ◽  
E. Elmore ◽  
D. K. Gulati ◽  
T. R. Barfknecht ◽  
...  
Keyword(s):  
Chinese Hamster ◽  
Metabolic Cooperation ◽  
Interlaboratory Studies ◽  
V79 Cell

Further characterization of the in vitro assay for inhibitors of metabolic cooperation in the Chinese hamster V79 cell line

1985 ◽  
Vol 6 (3) ◽  
pp. 361-366 ◽  
Author(s):  
C. Jone ◽  
J.E. Trosko ◽  
C.F. Aylsworth ◽  
L. Parker ◽  
C.C. Chang
Keyword(s):  
Cell Line ◽  
Chinese Hamster ◽  
In Vitro Assay ◽  
Metabolic Cooperation ◽  
Vitro Assay ◽  
V79 Cell

Elimination of metabolic cooperation in Chinese hamster cells by a tumor promoter

Science ◽  
1979 ◽  
Vol 206 (4422) ◽  
pp. 1089-1091 ◽  
Author(s):  
L. Yotti ◽  
C. Chang ◽  
J. Trosko
Keyword(s):  
Chinese Hamster ◽  
Tumor Promoter ◽  
Metabolic Cooperation ◽  
Chinese Hamster Cells

Transgenic chinese hamster V79 cell lines which exhibit variable levels ofgpt mutagenesis

1990 ◽  
Vol 16 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Catherine B. Klein ◽  
Toby G. Rossman ◽  
T. R. Skopek
Keyword(s):  
Cell Lines ◽  
Chinese Hamster ◽  
V79 Cell

Heterologous Expression of Mouse Cytochrome P450 2e1 in V79 Cells: Construction and Characterisation of the Cell Line and Comparison with V79 Cell Lines Stably Expressing Rat P450 2E1 and Human P450 2E1

2003 ◽  
Vol 31 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ulrike Bernauer ◽  
Hansruedi Glatt ◽  
Barbara Heinrich-Hirsch ◽  
Yungang Liu ◽  
Eva Muckel ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cell Line ◽  
Cell Lines ◽  
Active Form ◽  
Chinese Hamster ◽  
Established Cell Line ◽  
Cytochrome P450 2E1 ◽  
Hydroxylase Activity ◽  
Catalytically Active ◽  
V79 Cell

A V79 Chinese hamster cell line was constructed for stable expression of mouse cytochrome P450 2e1 (Cyp2e1), as an addition to the existing cell battery consisting of cell lines stably expressing rat CYP2E1 and human CYP2E1 (V79 Cell Battery™). The aim was to establish a cell battery that offers the in vitro possibility of investigating species-specific differences in the toxicity and metabolism of chemicals representing substrates for CYP2E1. The newly established cell line (V79m2E1) effectively expressed Cyp2e1 in the catalytically active form. The expression of catalytically active CYP2E1 in V79m2E1 cells was maintained over several months in culture, as demonstrated by Western Blotting and chlorzoxazone (CLX) 6-hydroxylase activity. The cells exhibited CLX 6-hydroxylase activity with a Km of 27.8μM/l and Vmax of 40pmol/mg protein/minute, compared with a Km of 28.2/28.6μM/l and a Vmax of 130/60pmol/mg protein/minute from V79r2E1/V79h2E1 cells. Furthermore, the CYP2E1-dependent mutagenicity of N-nitrosodimethylamine could be demonstrated in the V79m2E1 cells. Therefore, the new cell battery permits the interspecies comparison of CYP2E1-dependent toxicity and of metabolism of chemicals between humans and the two major rodent species — the rat and the mouse — that are usually used in classical toxicity studies.

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