Sister chromatid exchanges in chines hamster cells treated with seventeen organophosphorus compounds in the presence of a metabolic activation system

1982 ◽  
Vol 4 (5) ◽  
pp. 621-624 ◽  
Author(s):  
H. H. Chen ◽  
S. R. Sirianni ◽  
C. C. Huang
Keyword(s):  
Sister Chromatid ◽  
Organophosphorus Compounds ◽  
Metabolic Activation ◽  
Sister Chromatid Exchanges ◽  
Activation System ◽  
Chromatid Exchanges

scholarly journals Induction of sister chromatid exchanges by cyclophosphamide in human lymphocytes with metabolic activation.

1980 ◽  
Vol 56 (9) ◽  
pp. 568-572 ◽  
Author(s):  
Kiyoshi INOUE ◽  
Tadayoshi SHIBATA ◽  
Shinichi MISAWA ◽  
Tatsuo ABE ◽  
Keiichi KAWAI
Keyword(s):  
Sister Chromatid ◽  
Human Lymphocytes ◽  
Metabolic Activation ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges

Compound A Induces Sister Chromatid Exchanges in Chinese Hamster Ovary Cells 

1997 ◽  
Vol 86 (4) ◽  
pp. 918-922 ◽  
Author(s):  
Edmond I. Eger ◽  
Michael J. Laster ◽  
Richard Winegar ◽  
Christine Han ◽  
Diane Gong
Keyword(s):  
Sister Chromatid ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Metabolic Activation ◽  
Sister Chromatid Exchanges ◽  
Low Flow ◽  
Compound A ◽  
Ovary Cells ◽  
Chromatid Exchanges

Background Compound A [CF2 = C(CF3)OCH2F], a degradation product of sevoflurane [(CF3)2CHOCH2F], is a vinyl ether and may be an alkylating agent. Thus it is a potential genotoxin. Methods The capacity of compound A to produce sister chromatid exchanges was measured in Chinese hamster ovary cells with and without metabolic activation. Concentrations of 11 to 468 ppm compound A were applied for 2 h, the Chinese hamster ovary cells were incubated for a further 34 h in the presence of bromodeoxyuridine, and then colcemid was added to produce arrest in metaphase. Coded slides of cells were examined blindly, and 50 chromosome spreads were counted for each test concentration. Results The lowest concentration of compound A applied without metabolic activator (27 ppm) significantly increased (P < 0.001) sister chromatid exchanges, and increasing concentrations of compound A increased the incidence of exchanges. Metabolic activation did not increase the incidence of exchanges. Conclusions Compound A increases sister chromatid exchanges at concentrations (27 ppm) obtained in low-flow systems when sevoflurane is used at concentrations approximating minimum alveolar concentration.

Sign in / Sign up

Export Citation Format

Share Document