Mutagenic response to ultraviolet light and X-rays at five independent genetic loci in chinese hamster ovary cells

1982 ◽  
Vol 4 (5) ◽  
pp. 543-551 ◽  
Author(s):  
Bhag Singh ◽  
Radhey S. Gupta
Keyword(s):  
Ultraviolet Light ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
X Rays ◽  
Genetic Loci ◽  
Ovary Cells ◽  
Mutagenic Response

Effects of ultraviolet light on synchronized Chinese hamster ovary cells; potentiation by hydroxyurea

1977 ◽  
Vol 28 (1) ◽  
pp. 29-48
Author(s):  
K. Burg ◽  
A.R. Collins ◽  
R.T. Johnson
Keyword(s):  
Dna Synthesis ◽  
Ultraviolet Light ◽  
Hela Cells ◽  
Chinese Hamster Ovary ◽  
Ultraviolet Irradiation ◽  
Chromosome Structure ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

We have examined the effects of hydroxyurea on u.v.-irradiated Chinese hamster CHO-KI cells. Ultraviolet irradiation followed by incubation with hydroxyurea causes only slight disruption of DNA and chromosome structure in CHO-KI cells compared with HeLa cells. There is, however, a clear potentiation by hydroxyurea of the u.v. killing of CHO-KI cells, which is most pronounced at those points in the cycle which are reported to have small DNA precursor pools. This potentiation is reduced when DNA precursors are provided together with hydroxyurea. These data are discussed in terms of an uncoupling of excision and repair DNA synthesis.

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