The relative contributions of B and T lymphocytes in the human peripheral blood mutagen test system as determined by cell survival, mitogenic stimulation, and induction of chromosome aberrations by radiation

1980 ◽  
Vol 2 (4) ◽  
pp. 473-485 ◽  
Author(s):  
Jeffrey L. Schwartz ◽  
Mary Esther Gautden
Keyword(s):  
T Lymphocytes ◽  
Cell Survival ◽  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Human Peripheral Blood ◽  
Test System ◽  
Mitogenic Stimulation ◽  
B And T Lymphocytes

scholarly journals Differential expression of somatostatin receptor subtypes in human peripheral blood mononuclear cell subsets

2004 ◽  
pp. 565-577 ◽  
Author(s):  
EG Lichtenauer-Kaligis ◽  
VA Dalm ◽  
SP Oomen ◽  
DM Mooij ◽  
PM van Hagen ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Mrna Expression ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Lymphocyte Activation ◽  
Cell Types ◽  
Facs Analysis ◽  
Peripheral Blood Mononuclear ◽  
B And T Lymphocytes

BACKGROUND: Somatostatin (SS)-binding sites have been demonstrated in human lymphoid tissues and peripheral blood cells. However, not much is known with respect to the SS receptor subtype (sst) expression pattern and the expression of SS itself in the immune system. OBJECTIVE: The aim of this study was to evaluate the mRNA expression of the five known sst (sst(1-5)) in peripheral blood mononuclear cell (sub)populations. Moreover, the expression of the mRNAs encoding SS and the SS-like peptide cortistatin (CST) in immune cell subsets was studied. METHODS: RT-PCR and quantitative PCR were performed to evaluate sst, SS and CST mRNA expression in cells in the basal or activated state. Fluorescence-activated cell sorter (FACS) analysis using fluorescent SS was performed to visualize sst protein on cell membranes. RESULTS: B- and T-lymphocytes selectively expressed sst(3) mRNA. sst(3) expression in B-lymphocytes was significantly lower compared with T-lymphocytes. Unstimulated, freshly isolated monocytes did not express any sst mRNA. Upon activation, monocytes selectively expressed sst(2) mRNA, whereas T-lymphocyte activation upregulated sst(3) expression. sst(2) mRNA expression on monocytes was confirmed by FACS analysis. B- and T-lymphocytes did not express SS mRNA, while both cell types expressed CST mRNA. CST mRNA expression was downregulated following T-lymphocyte activation. CONCLUSION: We demonstrate for the first time unequivocally that human peripheral blood B- and T-lymphocytes selectively express sst(3), whereas monocytes do not express sst. However, upon activation, monocytes are induced to express sst(2A). No expression of SS mRNA was detected in any cell type, whereas all cell types expressed CST mRNA. The differential expression of sst and CST mRNA in lymphocytes and monocytes suggests a functional significance for the CST-sst interaction in immune cells, but further studies should be performed to evaluate the significance of sst and CST in these cells.

Identification, Enumeration, and Isolation of B and T Lymphocytes from Human Peripheral Blood.

1974 ◽  
Vol 3 (5) ◽  
pp. 521-532 ◽  
Author(s):  
F. Aiuti ◽  
J.C. Cerottini ◽  
R. R. A. Coombs ◽  
M. Cooper ◽  
H. B. Dickler ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
B And T Lymphocytes

scholarly journals Diacylglycerol activates the influx of extracellular cations in T-lymphocytes independently of intracellular calcium-store depletion and possibly involving endogenous TRP6 gene products

2002 ◽  
Vol 364 (1) ◽  
pp. 245-254 ◽  
Author(s):  
Alessandra GAMBERUCCI ◽  
Emanuele GIURISATO ◽  
Paola PIZZO ◽  
Maristella TASSI ◽  
Roberta GIUNTI ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Transient Receptor Potential ◽  
Human Peripheral Blood ◽  
Receptor Potential ◽  
Animal Cells ◽  
Bivalent Cation ◽  
Intracellular Calcium Store ◽  
Transient Receptor

In Jurkat and human peripheral blood T-lymphocytes, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol, activated the influx of Ca2+, Ba2+ and Sr2+. OAG also caused plasma-membrane depolarization in Ca2+-free media that was recovered by the addition of bivalent cation, indicating the activation of Na+ influx. OAG-induced cation influx was (i) mimicked by the natural dacylglycerol 1-stearoyl-2-arachidonyl-sn-glycerol, (ii) not blocked by inhibiting protein kinase C or in the absence of phopholipase C activity and (iii) blocked by La3+ and Gd3+. Differently from OAG, both thapsigargin and phytohaemagglutinin activated a potent influx of Ca2+, but little influx of Ba2+ and Sr2+. Moreover, the influx of Ca2+ activated by thapsigargin and that activated by OAG were additive. Furthermore, several drugs (i.e. econazole, SKF96365, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, 2-aminoethoxy diphenylborate and calyculin-A), while inhibiting the influx of Ca2+ induced by both thapsigargin and phytohaemagglutinin, did not affect OAG-stimulated cation influx. Transient receptor potential (TRP) 3 and TRP6 proteins have been shown previously to be activated by diacylglycerol when expressed heterologously in animal cells [Hofmann, Obukhov, Schaefer, Harteneck, Gudermann and Schultz (1999) Nature (London) 397, 259–263]. In both Jurkat and peripheral blood T-lymphocytes, mRNA encoding TRP proteins 1, 3, 4 and 6 was detected by reverse transcriptase PCR, and the TRP6 protein was detected by Western blotting in a purified plasma-membrane fraction. We conclude that T-cells express a diacylglycerol-activated cation channel, unrelated to the channel involved in capacitative Ca2+ entry, and associated with the expression of TRP6 protein.

Differential chemokine expression profiles in human peripheral blood T lymphocytes: dependence on T-cell coreceptor and calcineurin signaling

Blood ◽  
2003 ◽  
Vol 101 (1) ◽  
pp. 216-225 ◽  
Author(s):  
Anthony D. Cristillo ◽  
Mirtha J. Macri ◽  
Barbara E. Bierer
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Signaling Pathways ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Expression Profiles ◽  
Lymphoid Tissues ◽  
Rnase Protection ◽  
Chemokine Expression ◽  
Calcineurin Activity

Abstract The chemokine superfamily consists of small (8-10 kDa) molecules that function to attract, selectively, different subsets of leukocytes. Binding of chemokines to their appropriate G-protein–coupled receptors is necessary for primary immune responses and for homing of leukocytes to lymphoid tissues. Here, we have characterized the signaling pathways in primary T lymphocytes that regulate chemokine gene induction using an RNase protection assay. Dependence on stimulation through the coreceptor CD28 and sensitivity to the calcineurin inhibitors cyclosporine and tacrolimus were studied using purified human peripheral blood lymphocytes. Lymphotactin (Ltn), macrophage inflammatory protein (MIP)–1α, and MIP-1β were all rapidly induced and sensitive to cyclosporine treatment. At later time points, the expression of MIP-1α and MIP-1β, but not of Ltn, was restored despite the inhibition of calcineurin activity. By contrast, the induction of interleukin-8 was delayed and was found to be cyclosporine insensitive. Calcineurin activity of IP-10 mRNA induction was contingent on the specific T-cell stimulation conditions, suggesting that IP-10 expression is modulated by calcineurin-dependent and -independent signaling pathways. Differential chemokine expression profiles result from the engagement of T-cell coreceptors and the requirement for, and the dependence on, calcineurin phosphatase activity.

scholarly journals Expression of IκBα in the nucleus of human peripheral blood T lymphocytes

Oncogene ◽  
1999 ◽  
Vol 18 (8) ◽  
pp. 1581-1588 ◽  
Author(s):  
Teresa Laín de Lera ◽  
Lola Folgueira ◽  
Angel G Martín ◽  
Catherine Dargemont ◽  
María-Antonia Pedraza ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood

scholarly journals Derivation of Induced Pluripotent Stem Cells from Human Peripheral Blood T Lymphocytes

PLoS ONE ◽  
2010 ◽  
Vol 5 (6) ◽  
pp. e11373 ◽  
Author(s):  
Matthew E. Brown ◽  
Elizabeth Rondon ◽  
Deepika Rajesh ◽  
Amanda Mack ◽  
Rachel Lewis ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Lymphocytes ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Induced Pluripotent

TCR γ Chain Expression on Human Peripheral Blood T Lymphocytes

1989 ◽  
pp. 551-553
Author(s):  
Frits Koning ◽  
Rafick P. Sekaly ◽  
Erwin Tschachler ◽  
Roberto Biassoni ◽  
Marvin S. Reitz ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood

EFFECT OF ORAL ANTICOAGULANTS ON STABLE ROSETTE FORMATION

1987 ◽  
Author(s):  
S Aydar ◽  
S Alataş ◽  
L Numanoğlu ◽  
A Sönmezdağ
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Oral Anticoagulants ◽  
Peripheral Blood Lymphocytes ◽  
Culture Conditions ◽  
Rosette Formation ◽  
Normal Children ◽  
Activated Lymphocytes ◽  
Heat Stable

Human peripheral blood T lymphacytes when cultered in the presence of mitogen Phytohemogglutinin (PHA) acquire the capacity to form E rosettes with sheep erythrocytes that are resistant to incubation at 37° C. Whereas human thymus lymphocytes form 37° C stable E rosettes. On the other hand, it is shown that the use of anticoagulants can prevent cancer metastases which brings forth the importance of explaining the relationship between the lymphocyte functions and anticoagulant action mecha-nismus. In order to investigate this relationship, we did a group af experiments with lymphocytes of normal children and of children with severe burn wounds. Peripheral blood lymphocytes were seperated by “Lymphoprep” centrifugation technique. The lymphocytes of normal children and patients with burn were divided in two groups: A-Activated lymphocytes: 1×106 /ml lymphocytes were cultured and activated by PHAfor 48 hours at 37° C in RPMI 1640. B-Non activated lymphocytes were in culture witout PHA. 1×10™6 M/ml warfarin sulfate was added to some of the cultures of each group prior to the culture conditions. At the end of the 48 hour incubation, heat stable rosette formation was determined by the method of Wauve and co-workers. Significantly elevated levels of heat stable rosette forming cells were found in the PHA activated culture treated with warfarin sulfate in normals and patients with burn. Although the blastic transformation of T lymphocytes was found to be depressed, heat stable rosette formation of warfarin sulfate treated lymphocytes abtained from burn patients was observed to be significantly elevated. It is concluded that warfarin sulfate increases the activity of T lymphocytes by interfering with the resynthesis of heai stable E receptors.

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