In vitro BALB/3T3 cell transformation assay of nonoxynol-9 and 1,4-dioxane

1988 ◽  
Vol 11 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Chingju W. Sheu ◽  
Frances M. Moreland ◽  
Jung Keun Lee ◽  
Virginia C. Dunkel
Keyword(s):  
Cell Transformation ◽  
Transformation Assay

scholarly journals Comparative tumor promotion assessment of e-cigarette and cigarettes using the in vitro Bhas 42 cell transformation assay

2017 ◽  
Vol 58 (4) ◽  
pp. 190-198 ◽  
Author(s):  
Damien Breheny ◽  
Oluwatobiloba Oke ◽  
Kamala Pant ◽  
Marianna Gaça
Keyword(s):  
Cell Transformation ◽  
Tumor Promotion ◽  
Transformation Assay

scholarly journals Comparison of In Vitro Cell Transformation Assay Using Murine Fibroblasts and Human Keratinocytes

2008 ◽  
Vol 24 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Jun-Ho Ahn ◽  
Sue-Nie Park ◽  
Yung-Na Yum ◽  
Ji-Young Kim ◽  
Michael Lee
Keyword(s):  
Cell Transformation ◽  
Human Keratinocytes ◽  
Transformation Assay ◽  
Murine Fibroblasts

scholarly journals Cadmium-transformed cells in the in vitro cell transformation assay reveal different proliferative behaviours and activated pathways

2016 ◽  
Vol 36 ◽  
pp. 71-80 ◽  
Author(s):  
M. Forcella ◽  
G. Callegaro ◽  
P. Melchioretto ◽  
L. Gribaldo ◽  
M. Frattini ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Transformed Cells ◽  
Transformation Assay

Toxicogenomics applied to in vitro Cell Transformation Assay reveals mechanisms of early response to cadmium

2018 ◽  
Vol 48 ◽  
pp. 232-243 ◽  
Author(s):  
Giulia Callegaro ◽  
Matilde Forcella ◽  
Pasquale Melchioretto ◽  
Annalisa Frattini ◽  
Laura Gribaldo ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Early Response ◽  
Transformation Assay

Use of the Syrian Hamster Embryo (SHE) Cell Transformation Assay for the In Vitro Testing of Five Pesticides

1993 ◽  
Vol 21 (2) ◽  
pp. 202-205
Author(s):  
Maria Dušinská ◽  
Darina Slamen˘ova ◽  
Ada Kolman
Keyword(s):  
Syrian Hamster ◽  
Positive Response ◽  
Cell Transformation ◽  
Positive Control ◽  
In Vitro Testing ◽  
Morphological Transformation ◽  
Transformation Assay ◽  
Transforming Activity

The morphological transformation of Syrian hamster embryo (SHE) cells has been used for the in vitro testing of the transforming activity of five pesticides (Dual, Vucht 524, Piritione, Decemtione and Supercypermethrin). Morphological transformation with two known inducers, 3-methylcholanthrene and N-methyl- N‘-nitro- N-nitrosoguanidine, was used as a positive control. It was found that only one of the tested pesticides, Dual, failed to induce morphological transformation in SHE cells, whereas the remaining four demonstrated varying degrees of positive response and could be regarded as potential carcinogenic chemicals.

Effects of diazepam in mutation test systems in vitro and in the BHK 21 cell transformation assay

1984 ◽  
Vol 130 (3) ◽  
pp. 260-261
Author(s):  
G. Röhrborn ◽  
C. Thiel ◽  
D. Heimbach ◽  
M. Manolache ◽  
J. Gebauer
Keyword(s):  
Cell Transformation ◽  
Test Systems ◽  
Transformation Assay

scholarly journals Synthesis and in vitro characterization of the genotoxic, mutagenic and cell-transforming potential of nitrosylated heme

2020 ◽  
Vol 94 (11) ◽  
pp. 3911-3927 ◽  
Author(s):  
Tina Kostka ◽  
Jörg Fohrer ◽  
Claudia Guigas ◽  
Karlis Briviba ◽  
Nina Seiwert ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Malignant Cell ◽  
Red Meat ◽  
Colorectal Carcinogenesis ◽  
In Vivo Studies ◽  
Processed Meat ◽  
Cell Transforming ◽  
Malignant Cell Transformation

Abstract Data from epidemiological studies suggest that consumption of red and processed meat is a factor contributing to colorectal carcinogenesis. Red meat contains high amounts of heme, which in turn can be converted to its nitrosylated form, NO-heme, when adding nitrite-containing curing salt to meat. NO-heme might contribute to colorectal cancer formation by causing gene mutations and could thereby be responsible for the association of (processed) red meat consumption with intestinal cancer. Up to now, neither in vitro nor in vivo studies characterizing the mutagenic and cell transforming potential of NO-heme have been published due to the fact that the pure compound is not readily available. Therefore, in the present study, an already existing synthesis protocol was modified to yield, for the first time, purified NO-heme. Thereafter, newly synthesized NO-heme was chemically characterized and used in various in vitro approaches at dietary concentrations to determine whether it can lead to DNA damage and malignant cell transformation. While NO-heme led to a significant dose-dependent increase in the number of DNA strand breaks in the comet assay and was mutagenic in the HPRT assay, this compound tested negative in the Ames test and failed to induce malignant cell transformation in the BALB/c 3T3 cell transformation assay. Interestingly, the non-nitrosylated heme control showed similar effects, but was additionally able to induce malignant transformation in BALB/c 3T3 murine fibroblasts. Taken together, these results suggest that it is the heme molecule rather than the NO moiety which is involved in driving red meat-associated carcinogenesis.

scholarly journals Chondroitin Sulphate Proteoglycan 4 (NG2/CSPG4) Localization in Low- and High-Grade Gliomas

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1538 ◽  
Author(s):  
Marta Mellai ◽  
Laura Annovazzi ◽  
Ilaria Bisogno ◽  
Cristiano Corona ◽  
Paola Crociara ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Chondroitin Sulphate ◽  
Molecular Genetic ◽  
Malignant Gliomas ◽  
Malignant Cell ◽  
Wild Type ◽  
Sulphate Proteoglycan ◽  
Chondroitin Sulphate Proteoglycan ◽  
Proteoglycan 4

Background: Neuron glial antigen 2 or chondroitin sulphate proteoglycan 4 (NG2/CSPG4) is expressed by immature precursors/progenitor cells and is possibly involved in malignant cell transformation. The aim of this study was to investigate its role on the progression and survival of sixty-one adult gliomas and nine glioblastoma (GB)-derived cell lines. Methods: NG2/CSPG4 protein expression was assessed by immunohistochemistry and immunofluorescence. Genetic and epigenetic alterations were detected by molecular genetic techniques. Results: NG2/CSPG4 was frequently expressed in IDH-mutant/1p19q-codel oligodendrogliomas (59.1%) and IDH-wild type GBs (40%) and rarely expressed in IDH-mutant or IDH-wild type astrocytomas (14.3%). Besides tumor cells, NG2/CSPG4 immunoreactivity was found in the cytoplasm and/or cell membranes of reactive astrocytes and vascular pericytes/endothelial cells. In GB-derived neurospheres, it was variably detected according to the number of passages of the in vitro culture. In GB-derived adherent cells, a diffuse positivity was found in most cells. NG2/CSPG4 expression was significantly associated with EGFR gene amplification (p = 0.0005) and poor prognosis (p = 0.016) in astrocytic tumors. Conclusion: The immunoreactivity of NG2/CSPG4 provides information on the timing of the neoplastic transformation and could have prognostic and therapeutic relevance as a promising tumor-associated antigen for antibody-based immunotherapy in patients with malignant gliomas.

scholarly journals Direct repeats bind the EcR/USP receptor and mediate ecdysteroid responses in Drosophila melanogaster.

1996 ◽  
Vol 16 (6) ◽  
pp. 2977-2986 ◽  
Author(s):  
C Antoniewski ◽  
B Mugat ◽  
F Delbac ◽  
J A Lepesant
Keyword(s):  
Fat Body ◽  
Cell Transformation ◽  
Direct Repeat ◽  
Transgenic Animals ◽  
Transcriptional Level ◽  
Regulatory Sequences ◽  
Direct Repeats ◽  
Fbp1 Gene

The steroid hormone 20-hydroxyecdysone plays a key role in the induction and modulation of morphogenetic events throughout Drosophila development. Previous studies have shown that a heterodimeric nuclear receptor composed of the EcR and USP proteins mediates the action of the hormone at the transcriptional through binding to palindromic ecdysteroid mediates the action of the hormone at the transcriptional level through binding to palindromic ecdysteroid response elements (EcREs) such as those present in the promoter of the hsp27 gene or the fat body-specific enhancer of the Fbp1 gene. We show that in addition to palindromic EcREs, the EcR/USP heterodimer can bind in vitro with various affinities to direct repetitions of the motif AGGTCA separated by 1 to 5 nucleotides (DR1 to DR5), which are known to be target sites for vertebrate nuclear receptors. At variance with the receptors, EcR/USP was also found to bind to a DR0 direct repeat with no intervening nucleotide. In cell transformation assays, direct repeats DR0 to DR5 alone can render the minimum viral tk or Drosophila Fbp1 promoter responsive to 20-hydroxyecdysone, as does the palindromic hsp27 EcRE. In a transgenic assay, however, neither the palindromic hsp27 element nor direct repeat DR3 alone can make the Fbp1 minimal promoter responsive to premetamorphic ecdysteroid peaks. In contrast, DR0 and DR3 elements, when substituted for the natural palindromic EcRE in the context of the Fbp1 enhancer, can drive a strong fat body-specific ecdysteroid response in transgenic animals. These results demonstrate that directly repeated EcR/USP binding sites are as effective as palindromic EcREs in vivo. They also provide evidence that additional flanking regulatory sequences are crucially required to potentiate the hormonal response mediated by both types of elements and specify its spatial and temporal pattern.

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